Errors in spectrophotometry and calibration procedures to avoid them

Reule, A.

doi:10.6028/jres.080a.060

Cited by 46 publications

(16 citation statements)

References 10 publications

(21 reference statements)

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“…cantalupensis L.) [17]. The UV-Vis spectrophotometry tool has a high degree of accuracy in analyzing a sample where this tool is a method for testing the amount of light absorbed in each wavelength in the ultraviolet area, which works by forwarding the light transmitted by blank and sample detectors [18].…”

Section: Raw Jongimentioning

confidence: 99%

Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

Nisa¹,

Walanda²

2021

World J. Adv. Res. Rev.

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Beta-carotene is converted in the body to vitamin A, a powerful antioxidant that plays an essential role in maintaining healthy eyes, skin, and neurological function. Beta-carotene and other carotenoids have antioxidant activities and are valued for their ability to prevent chronic disease. β-carotene is found in yellow, orange, red, and green fruits. The purpose of this study was to determine the β-carotene in Jongi. Jongi (Dillenia Serrata Thunb.) is an endemic fruit of Sulawesi which contains β-carotene. The samples used in this study were raw jongi and ripe jongi extracted with n-hexane: acetone (1:4). To the samples were analyzed quantitatively using UV-Vis spectrophotometry at a wavelength of 450.00 nm. The results showed that the β-carotene in raw jongi was 0.3554 mg/100 g and the β- carotene in ripe jongi was 1.1841 mg/100 g. Ripe jongi consist of more β-carotene than the raw jongi, hence better as a source of Vitamin A.

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Section: Raw Jongimentioning

confidence: 99%

Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

Nisa¹,

Walanda²

2021

World J. Adv. Res. Rev.

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“…Stray light, from instrument malfunction, diffraction, or light scattering, decreases the measurable absorbance range and alters the relationship between concentration and absorbance. It includes heterochromatic electromagnetic radiation outside the bandpass of the monochromator …”

Section: Uncertaintymentioning

confidence: 99%

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

et al. 2018

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UV-vis spectroscopy is an inexpensive, simple, flexible, non-destructive, analytical method appropriate for a wide class of organic compounds and some inorganic species. UV-vis spectrophotometers measure the absorbance or transmittance of light passing through a medium as a function of the wavelength. Chemical engineers apply it for quantitative analysis, to derive liquid phase reaction kinetics, and to identify the mechanism at the molecular scale. High performance liquid chromatography and ultra-high performance liquid chromatography integrate UV-vis detectors to identify and quantify the concentration of compounds in liquid streams. Combining these techniques with mass spectrometry facilitates identifying all species. UV-vis diffuse reflectance spectroscopy is a variant with enhanced scattering properties that measures the properties of solids and powders. A bibliometric analysis of the 10 000 most cited papers referring to UV-vis (2016 and 2017) groups research in four major clusters: nanoparticles and nanostucutres; photocatalysis and water treatment; crystals, complexes, and derivatives; and Ag and Au nanoparticles biological interaction.

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“…However, experimental verification is practically impossible, because microscope-photometric measurements are burdened by special uncertainties. These may have various sources (for details see Reule, 1976;Piller, 1977;Cadman & Sadowski, 1978): different optical and/or radiation-physical behaviour of the object and the reference medium, diffraction and/or refraction effects in the preparation, effects of reflections and/or stray-light inside and outside the preparation, inaccurate focusing, deficient reproducibility of measuring conditions during the measurements at the object and aberrations of the optical system, disturbances of the functions of photometric modules.…”

Section: Discussionmentioning

confidence: 99%

The influence of the aperture of illumination on the accuracy of microscope‐photometric measurements of internal transmittance

Boguth

Piller

1988

Journal of Microscopy

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SUMMARY The conical shape of the bundle of illuminating rays in the microscope causes a systematic error of transmittance measured with a microscope photometer in comparison with the (true) transmittance measured with a macrophotometer in which the ray bundle is cylindrical. The error is evaluated by the ratio of transmittance measured with the microscope photometer to the true transmittance. It is calculated for the following model objects: parallel‐sided plates, wedges, spheres and circular cylinders. The results of calculations show that the error increases with decreasing transmittance, increasing angle of the cone of rays (evaluated by the effective numerical aperture of illumination), and increasing relative size of photometric field. On the whole, the error can be neglected if the true internal transmittance is greater than 0.1 (10%), the numerical aperture is less than 0.45 and the radius of the photometric field (in the object plane) is less than one‐fifth of the thickness of the object.

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Errors in spectrophotometry and calibration procedures to avoid them

Cited by 46 publications

References 10 publications

Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

Analysis of βeta-carotene from Jongi (Dillenia Serrata Thunb.) as a source of vitamin A

Experimental methods in chemical engineering: Ultraviolet visible spectroscopy—UV‐Vis

The influence of the aperture of illumination on the accuracy of microscope‐photometric measurements of internal transmittance

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