Accumulation of scopoletin in Hevea brasiliensis infected by Microcyclus ulei (P. Henn.) V. ARX and evaluation of its fungitoxicity for three leaf pathogens of rubber tree

García, Dominique; Sanier, Christine; Macheix, Jean‐Jacques; D'Auzac, Jean

doi:10.1006/pmpp.1995.1053

Cited by 36 publications

(24 citation statements)

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“…The degree of clonal resistance appears to be directly related to the rate of coumarin accumulation. Quantification of scopoletin production confirms these observations (GARCIA et al 1995).…”

Section: Discussionsupporting

confidence: 71%

“…The substance seemed to be the only soluble phenol in the excudates collected from the abaxial surface of the leaves 12 h after inoculation. Quantitative determination of scopoletin in relation to resistance has been studied in another paper and was dependent on the degree of resistance of the clone (GARCIA et al 1995). However, traces of scopoletin were also produced in the controls.…”

Section: Identification Of Soluble Phenolsmentioning

confidence: 99%

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Chemical and structural barriers to Microcyclus ulei, the agent of South American leaf blight, in Hevea spp.

García

Cazaux

Rivano

et al. 1995

European Journal of Forest Pathology

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Summary Young leaves of different rubber‐tree clones were inoculated with Microcyclus ulei under controlled conditions. Under ultraviolet light, intense blue fluorescent light was emitted from the penetration site 6 h after inoculation. The speed of the appearance of this fluorescence was related to the degree of resistance of the clones: 12–36 h in totally resistant clones, 36–120 h in marked partially resistant clones, and 120 h in weak partially resistant clones. The source of this fluorescence may have been scopoletin. Strong accumulation of this coumarin may inhibit pathogen invasion in a very early phase. Four days after inoculation, intense accumulation of lignins in only a few cells at the penetration sites was observed in totally resistant clones. Intense lignin accumulation around lesions may also stop pathogen development in some partially resistant clones

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Section: Discussionsupporting

confidence: 71%

Section: Identification Of Soluble Phenolsmentioning

confidence: 99%

Chemical and structural barriers to Microcyclus ulei, the agent of South American leaf blight, in Hevea spp.

García

Cazaux

Rivano

et al. 1995

European Journal of Forest Pathology

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“…Breeding data (Garcia et al 2004), physiological results (Garcia et al 1995;Lieberei 2007), and genetic mapping locus (Lespinasse et al 2000) indicated that SALB resistance origin is largely conditioned by the plant genetics. Furthermore, recent description of the partial resistance of MDF180 cultivar indicated more severe SALB symptoms in controlled infection conditions than in natural field infection conditions (Le Guen et al 2008).…”

Section: Discussionmentioning

confidence: 97%

Foliar endophytic fungi from Hevea brasiliensis and their antagonism on Microcyclus ulei

et al. 2010

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Endophytic fungi live inside plants as symbionts for at least one period of their life cycle. They play an important role in plant defence and could be promising sources of valuable natural bioactive products. The present study aimed to select endophytic fungi antagonistic to Microcyclus ulei, the agent of South American Leaf Blight (SALB) responsible for the weak development of rubber plantations in Latin America. Endophytic fungi were isolated from leaves of three cultivars (FX3864, CDC312, MDF180) of Hevea brasiliensis (the rubber tree) with distinct resistance levels to the attack by Microcyclus ulei. Leaf samples were collected from a 5,000 ha area of the Michelin plantations (Igrapiúna, Bahia, Brazil). A total of 435 colonies of endophytic fungi were isolated, purified, and preserved in sterilized distilled water. Lyophilized culture filtrates obtained from 88 fungal isolates, grown in liquid malt extract medium, were tested in vitro at concentrations of 50% and 12.5% for their inhibitory potential against the germination of M. ulei PMB26 conidia. Thirteen filtered cultures of endophytic fungi that presented inhibitory activity above 80% (at the concentration 12.5%) were characterized taxonomically by sequencing the ITS regions of rDNA, revealing the following taxa: Fusarium sp., Gibberella sp., Glomerella cingulata, Microsphaeropsis sp., Myrothecium sp., Pestalotiopsis sp. and Phomopsis sp. Three taxa were detected only in the resistant cultivars: Microsphaeropsis sp., and Pestalotiopsis sp. in MDF180, and Myrothecium sp. in CDC312. The present work constitutes the first study showing inhibitory activity of rubber tree endophytic fungi on Microcyclus ulei. These results provide an encouraging basis for the identification of new bioactive molecules for an effective control of South American leaf blight.

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“…Moreover, the resistance of some plant species against fungal pathogens is correlated with the rapidity and intensity of scopoletin accumulation (Goy et al , 1993; Garcia et al , 1995; Churngchow and Rattarasarn, 2001; El Oirdi et al , 2010). These results represent a very important step in evaluating the defensive function of scopoletin; however, important experiments using scopoletin-depleted plants are still lacking.…”

Section: Introductionmentioning

confidence: 99%

Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling

Sun

Wang

Zhang

et al. 2014

Journal of Experimental Botany

136

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Accumulation of scopoletin in Hevea brasiliensis infected by Microcyclus ulei (P. Henn.) V. ARX and evaluation of its fungitoxicity for three leaf pathogens of rubber tree

Cited by 36 publications

References 0 publications

Chemical and structural barriers to Microcyclus ulei, the agent of South American leaf blight, in Hevea spp.

Chemical and structural barriers to Microcyclus ulei, the agent of South American leaf blight, in Hevea spp.

Foliar endophytic fungi from Hevea brasiliensis and their antagonism on Microcyclus ulei

Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling

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