Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense

Meza, Beatriz; de‐Bashan, Luz E.; Hernández, Juan-Pablo; Bashan, Yoav

doi:10.1016/j.resmic.2015.03.001

Cited by 31 publications

(12 citation statements)

References 79 publications

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“…A considerable number of previous studies reported that such a stimulation was caused by storage of excess P, under either autotrophic or heterotrophic cultivations [4, 14, 39, 40], where nitrogen was either limited or unlimited [41]. Excessive P induced improvements on supplying phospholipid, genetic materials, and energy for cell division [42, 43]. However, further increasing of the P supply could result in microalgal growth inhibition.…”

Section: Resultsmentioning

confidence: 99%

Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation

Yan

et al. 2019

Biotechnol Biofuels

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Background Phosphorus (P) is an essential element of microalgae, which is either required for anabolism or for energy metabolism. When employing a nitrogen limitation strategy to trigger microalgal intracellular lipid accumulation, P supplementation was always simultaneously applied to compensate for the accompanied growth inhibition. Results This study identified that P exerts hormesis effects on microalgae. Slight excess of P (≤ 45 mg L −1 ) under nitrogen limitation condition stimulated the cell growth of Chlorella regularis and achieved a 10.2% biomass production increase. This also improved mitochondrial activity by 25.0% compared to control (P = 5.4 mg L −1 ). The lipid productivity reached 354.38 mg (L d) −1 , which increased by 39.3% compared to control. Such an improvement was caused by the intracellularly stored polyphosphate energy pool. However, large excess of P (250 mg L −1 ) inhibited the cell growth by 38.8% and mitochondrial activity decreased by 71.3%. C. regularis cells showed obvious poisoning status, such as enlarged size, plasmolysis, deformation of cell walls, and disorganization of organelles. This is probably because the over-accumulated P protonated the amide-N and disrupted membrane permeability. Conclusions These results provide new insight into the roles of P in microalgae lipid production: P does not always play a positive role under nitrogen limitation conditions. Electronic supplementary material The online version of this article (10.1186/s13068-019-1458-z) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation

Yan

et al. 2019

Biotechnol Biofuels

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“…Indole Butirat Acid (IBA) adalah hormon auksin sintetis sedangkan auksin alami dihasilkan oleh mikroba seperti Azospirillum. Azospirillum adalah mikroba tanah yang hidup di daerah perakaran yang menghasilkan hormon IAA yang mengandung auksin dan mampu meningkatkan serapan hara termasuk pospor (Meza et. al., 2015).…”

Section: Gambar 4 Tahapan Penyambungan DI Pembibitan Untuk Mendapatkunclassified

TEKNOLOGI PENYAMBUNGAN (GRAFTING) MENDUKUNG PENGEMBANGAN, PEREMAJAAN DAN REHABILITASI PERTANAMAN JAMBU METE / Grafting Technology for Support Extensification, Replanting, and Rehabilitation of Cashew Plantation

Suryadi

2019

PSP

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Initially cashew cultivation was aimed at marginal land conservation. A tight spacing (3 m x 3 m) so that the crown can cover the ground as quickly as possible to prevent soil erosion during rain and deciduous cashew leaves will add soil organic matter. Therefore aspects of plant productivity have not been a concern. As the price of cashew nuts tends to increase every year. This can encourage farmers to try cashew crops more seriously. At present, cashew cultivation is not only an effort to conserve marginal land, but also as a source of income for farmers, especially in Eastern Indonesia (KTI). Until 2016, Indonesia's cashew area had reached 514,491 ha with production of 137,094 tons. However, the level of productivity is considered still low, namely 430 kg logs/ha/year, far below India and Nigeria in the range of 900-2,286 kg logs/ha/year. Some factors that cause low productivity of Indonesian cashew are (1) the quality of plant material used, (2) disruption of pests and diseases, (3) maintenance of plants, and (4) the number of old plants (> 30 years). For this reason, efforts need to be made to increase the productivity of cashew, by implementing grafting technology in the extensification, replanting and rehabilitation of cashew. Research related to the grafting has been done quite a lot and produced, both grafting in nurseries and directly on the field (top working). The production potential of 9 superior varieties released ranged from 5.97 - 37.44 kg logs/trees/year or an average of 16.70 kg logs/trees/year. If the extensification, replanting and rehabilitation activities apply the grafting technology using the stem from superior varieties, it will be able to increase the productivity of Indonesian cashew to 1,670 kg logs/ha/year or increase by 300% from current productivity. AbstrakAwalnya penanaman jambu mete bertujuan untuk konservasi lahan marjinal. Jarak tanam rapat (3 m x 3 m) agar tajuk dapat secepat mungkin menutup permukaan tanah untuk mencegah erosi permukaan tanah saat hujan dan daun jambu mete yang gugur akan menambah bahan organik tanah. Oleh sebab itu aspek produktivitas tanaman belum menjadi perhatian. Seiring perkembangan harga kacang mete cenderung meningkat setiap tahunnya. Hal tersebut mampu mendorong petani untuk mengusahakan tanaman jambu mete lebih serius. Saat ini penanaman jambu mete tidak hanya sebagai usaha konservasi lahan marjinal, namun menjadi sumber pendapatan petani, terutama di Kawasan Timur Indonesia (KTI). Sampai 2016, luas areal mete Indonesia telah mencapai 514.491 ha dengan produksi 137.094 ton. Namun, tingkat produktivitas dianggap masih rendah yaitu 430 kg gelondong/ha/tahun, jauh dibawah India dan Nigeria pada kisaran 900-2.286 kg gelondong/ha/tahun. Beberapa faktor yang menyebabkan rendahnya produktivitas jambu mete Indonesia yaitu (1) mutu bahan tanaman yang digunakan, (2) gangguan hama dan penyakit, (3) pemeliharaan tanaman, dan (4) banyaknya tanaman tua (>30 tahun). Untuk itu perlu ditempuh upaya meningkatkan produktivitas jambu mete, dengan menerapkan teknologi penyambungan pada kegiatan pengembangan, peremajaan dan rehabilitasi pertanaman jambu mete. Penelitian terkait penyambungan sudah cukup banyak dilakukan dan dihasilkan, baik penyambungan di pembibitan maupun langsung di lapang (top working). Potensi produksi dari 9 varietas unggul yang dilepas berkisar antara 5,97- 37,44 kg gelondong/pohon/tahun atau rata-rata 16,70 kg gelondong/pohon/tahun. Apabila kegiatan pengembangan, peremajaan dan rehabilitasi menerapkan teknologi penyambungan menggunakan batang atas dari varietas unggul, akan mampu meningkatkan produktivitas jambu mete Indonesia menjadi 1.670 kg gelondong/ha/tahun atau meningkat 300 % dari produktivitas saat ini.

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“…In contrast to NH3-N, removal efficiencies of PO4 3--P was affected in the presence of high concentration of tetracycline (Table 1). Earlier studies had shown that many microalgae including Chlorella vulgaris are able to accumulate intracellular polyphosphate [50,51]. Since the eukaryotic microalgae and biomass were not significantly perturbed by the presence of antibiotics, it is unlikely that the deterioration in PO4 3--P removal efficiencies was due to detrimental impact on microalgae.…”

Section: Differences In 16s Rrna Gene-based Communities Among Reactorsmentioning

confidence: 99%

Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors

et al. 2018

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This study investigated the impact of varying concentrations of tetracycline on the performance of mixed microalgae-bacteria photo-bioreactors. Photo-bioreactors were assessed for their ability to remove carbon dioxide (CO2) from the biogas of anaerobic membrane bioreactor (anMBR), and nutrients from the anaerobic effluent. The varying concentrations of tetracycline had no impact on the removal of CO2 from biogas. 29% v/v of CO2 was completely removed to generate > 20% v/v of oxygen (O2) in all reactors. Removal of nutrients and biomass was not affected at low concentrations of tetracycline (<=150 µg/L), but 20 mg/L of tetracycline lowered the biomass generation and removal efficiencies of phosphate. Conversely, high chlorophyll a and b content was observed at 20 mg/L of tetracycline. High tetracycline level had no impact on the diversity of 18S rRNA gene-based microalgal communities but adversely affected the 16S rRNA gene-based microbial communities. Specifically, both Proteobacteria and Bacteroidetes phyla decreased in relative abundance but not phylum Chloroplast. Additionally, both nitrogenfixing (e.g. Flavobacterium, unclassified Burkholderiales and unclassified Rhizobiaceae) and denitrifying groups (e.g. Hydrogenophaga spp.) were significantly reduced in relative abundance at high tetracycline concentration. Phosphate-accumulating microorganisms, Acinetobacter spp. and Pseudomonas spp. were similarly reduced upon exposure to high tetracycline concentration. Unclassified Comamonadaceae, however, increased in relative abundance, which correlated with an increase in the abundance of tetracycline resistance genes associated with efflux pump mechanism. Overall, the findings demonstrate that antibiotic concentrations in municipal wastewaters will not significantly affect the removal of nutrients by the mixed microalgaebacteria photo-bioreactors. However, utilizing such photo-bioreactors as a polishing step for anMBRs that treat wastewaters with high tetracycline concentration may not be effective as evidenced from the lower nutrient removal and occurrence of antibiotic resistance genes. Highlights • Microalgal biomass yield and removal of CO2 from biogas unaffected by tetracycline • Removal of nutrient from anaerobic effluent affected by 20 mg/L of tetracycline • Microalgae community insignificantly affected by 20 mg/L of tetracycline • Bacterial populations, including those involved in N and P cycling, affected by 20 mg/L tetracycline

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Accumulation of intra-cellular polyphosphate in Chlorella vulgaris cells is related to indole-3-acetic acid produced by Azospirillum brasilense

Cited by 31 publications

References 79 publications

Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation

Hormesis effects of phosphorus on the viability of Chlorella regularis cells under nitrogen limitation

TEKNOLOGI PENYAMBUNGAN (GRAFTING) MENDUKUNG PENGEMBANGAN, PEREMAJAAN DAN REHABILITASI PERTANAMAN JAMBU METE / Grafting Technology for Support Extensification, Replanting, and Rehabilitation of Cashew Plantation

Increasing tetracycline concentrations on the performance and communities of mixed microalgae-bacteria photo-bioreactors

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