Accumulation of<i>N</i><sup>ε</sup>-(Hexanoyl)lysine, an Oxidative Stress Biomarker, in Rice Seeds during Storage

Minato, Ken-ichiro; Gono, Miki; Yamaguchi, Hiroko; Kato, Yoji; Osawa, Toshihiko

doi:10.1271/bbb.69.1806

Cited by 8 publications

(10 citation statements)

References 21 publications

(22 reference statements)

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“…There were not significant differences ( p < 0.005) among each value in Mn-SOD activities (This referred to Minato et al (2005) reprinted by permission of Japan Society for Bioscience, Biotechnology, and Agrochemistry) product, and a powerful marker indicating oxidative stress in animal tissue in which lipid peroxidation has been induced. We could show an accumulation of HEL in rice stressed oxidatively at high temperature.…”

Section: Resultsmentioning

confidence: 99%

“…6.2). Minato et al (2005) reprinted by permission of Japan Society for Bioscience, Biotechnology, and Agrochemistry) In the rice stored at 5 C, it did not change.…”

Section: A Quality Deterioration In Rice By Oxidative Stressmentioning

confidence: 99%

“…Values designated by different superscript letters are significantly different ( p < 0.005) (This referred to Minato et al (2005) reprinted by permission of Japan Society for Bioscience, Biotechnology, and Agrochemistry) remains obscure whether HEL directly influenced the deterioration in rice germination ability. Each value is presented as the mean AE S.D.…”

Section: Accumulation Of Hel In Rice Seed Induced Oxidative Stress Damentioning

confidence: 99%

“…It was initiated by the addition of sufficient xanthine oxidase to produce a basal rate of ferricytochrome c reduction corresponding to an increase in A 550 of 0.025. Values designated by different letters are significantly different ( p < 0.005) (This referred to Minato et al (2005) reprinted by permission of Japan Society for Bioscience, Biotechnology, and Agrochemistry) Activated oxygen-scavenging enzymes have received a great deal of attention in relation to various kinds of stresses. Mn-SOD activity was determined as described above, except that the assay mixture contained 2 mM KCN to inactivate Cu, Zn-SOD.…”

Section: Antioxidant Enzymes Activities In Oxidative Stressmentioning

confidence: 99%

“…Values designated by different letters are significantly different ( p < 0.005) (This referred toMinato et al (2005) reprinted by permission of Japan Society for Bioscience, Accumulation of TBARS in the rice during storage at 5 C (□) and 40 C (■ Each value is presented as the mean AE S.D.…”

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confidence: 99%

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Lipid Hydroperoxide-Derived Modification of Biomolecules

Kato¹

2014

Subcellular Biochemistry

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Section: Resultsmentioning

confidence: 99%

“…6.2). Minato et al (2005) reprinted by permission of Japan Society for Bioscience, Biotechnology, and Agrochemistry) In the rice stored at 5 C, it did not change.…”

Section: A Quality Deterioration In Rice By Oxidative Stressmentioning

confidence: 99%

Section: Accumulation Of Hel In Rice Seed Induced Oxidative Stress Damentioning

confidence: 99%

Section: Antioxidant Enzymes Activities In Oxidative Stressmentioning

confidence: 99%

mentioning

confidence: 99%

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Lipid Hydroperoxide-Derived Modification of Biomolecules

Kato¹

2014

Subcellular Biochemistry

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Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications

Kato

Osawa

2009

Lipidomics

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An amide-type adduct, hexanoyl-lysine (HEL) is generated from the reaction between n-6 fatty acid (FA)-derived lipid peroxide and lysine. Immunochemical and chemical methods can be used to detect the formation of HEL. For example, an ELISA kit using the monoclonal antibody to HEL is now commercially available. We recently identified propanoyl-lysine (propionyl-lysine, PRL) from the reaction of an n-3 FA and a lysine residue. The antibody to PRL has been prepared and characterized. Using these monoclonal antibodies, the localization of adducts in tissues has been confirmed. Moreover, both amide-type adducts, HEL and PRL, can be simultaneously measured using liquid chromatography mass spectrometry (LC/MS/MS) with isotope dilution methods. The LC/MS/MS analysis reveals the rigid amounts of the adducts in human urine. Both the chemical and immunochemical methods are useful for the estimation of amide-type adducts in vivo.

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Hexanoyl-Lysine as a Deterioration Marker for Rice During Storage

Minato

2013

Lipid Hydroperoxide-Derived Modification of Biomolecules

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N(ε)-(hexanoyl)lysine (HEL) is known to be an oxidative lipid-decomposition product, and a powerful marker indicating oxidative stress in animal tissue. We investigated whether HEL could be useful as a marker in rice seeds damaged by oxidative stress during storage, as well as animals. We could show an accumulation of HEL in rice stored at high temperature (40 °C). This result significantly corresponded with an accumulation of TBARS. Rice germination deteriorated with non-enzymatic lipid peroxidation during storage at high temperature for a few months. These results suggested that a deterioration of rice germination ability resulted from oxidative damage caused by lipid peroxidation during storage. Moreover, HEL could become a useful marker for oxidative stress induced by lipid peroxidation. In addition, the activities of antioxidant enzymes, catalase and superoxide dismutase, significantly decreased in the rice seeds during storage at 40 °C. The relationship between accumulation of HEL and increases in antioxidant enzymes activities must be further studied. But, these results suggest that HEL might be a useful marker of oxidative stress in rice as well as in animals.

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Accumulation ofN^ε-(Hexanoyl)lysine, an Oxidative Stress Biomarker, in Rice Seeds during Storage

Cited by 8 publications

References 21 publications

Lipid Hydroperoxide-Derived Modification of Biomolecules

Lipid Hydroperoxide-Derived Modification of Biomolecules

Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications

Hexanoyl-Lysine as a Deterioration Marker for Rice During Storage

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Accumulation ofNε-(Hexanoyl)lysine, an Oxidative Stress Biomarker, in Rice Seeds during Storage

Cited by 8 publications

References 21 publications

Lipid Hydroperoxide-Derived Modification of Biomolecules

Lipid Hydroperoxide-Derived Modification of Biomolecules

Detection of a Lipid-Lysine Adduct Family with an Amide Bond as the Linkage: Novel Markers for Lipid-Derived Protein Modifications

Hexanoyl-Lysine as a Deterioration Marker for Rice During Storage

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Accumulation ofN^ε-(Hexanoyl)lysine, an Oxidative Stress Biomarker, in Rice Seeds during Storage