Accumulation of Eosinophils in the Infundibulum of the Bovine Oviduct Just after Ovulation

Abstract

This study was to investigate the localization and distribution of eosinophils (EOS) in the bovine oviduct throughout the estrous cycle. Histological studies revealed more abundant EOS in the infundibula of the oviducts ipsilateral to the preovulatory dominant follicle and the ovulated ovary. The number of EOS was higher in the infundibula of the oviducts ipsilateral to the ovulated ovary than those of the oviducts contralateral to the ovulated ovary. The infundibula of the oviducts ipsilateral to the preovulatory dominant follicle had higher number of EOS than those of the oviducts ipsilateral to the mid-cycle corpus luteum. The number of EOS in the isthmus, but not in the ampulla, was higher in the outer layers (tunica muscularis and tunica serosa) than in the inner layers (tunica mucosa and tunica submucosa) during the estrous cycle. Thus, the EOS number varied with the region of the bovine oviduct, with greater number in the infundibula of the oviduct ipsilateral to the ovulated ovary, suggesting the impact of ovulation.

Age-dependent changes in inflammation and extracellular matrix in bovine oviduct epithelial cells during the post-ovulatory phase

“…Oviducts were collected from Japanese Black cows at a local slaughterhouse within 10–20 min of exsanguination. The stage of the estrous cycle was determined based on macroscopic observations of the ovary and uterus, where healthy oviducts were selected (Miyamoto & Schams, ; Kowsar et al, ). Oviductal tissues ipsilateral to the corpus luteum were collected at Days 1–3 after ovulation ­ (which represents the phases of fertilization and early embryo development within the oviduct), transported to the laboratory (4°C, in phosphate‐buffered saline (PBS) containing antibiotics), and utilized for cell culture.…”

Palmitic acid stimulates interleukin‐8 via the TLR4/NF‐κB/ROS pathway and induces mitochondrial dysfunction in bovine oviduct epithelial cells

“…17 Oviductal tissues ipsilateral to the corpus luteum were collected at days 1-3 after ovulation, transported to the laboratory (4°C, in phosphate-buffered saline (PBS) containing antibiotics), and utilized for cell culture. The stage of the estrous cycle was determined based on macroscopic observations of the ovary and uterus.…”

“…Furthermore, oviducts as well as attached uteri were macroscopically examined to be healthy and free from any infections. 17 Oviductal tissues ipsilateral to the corpus luteum were collected at days 1-3 after ovulation, transported to the laboratory (4°C, in phosphate-buffered saline (PBS) containing antibiotics), and utilized for cell culture. For experiments, a total of 20 bovine oviductal tissues were obtained and isolated OECs as follows.…”

IL1B triggers inflammatory cytokine production in bovine oviduct epithelial cells and induces neutrophil accumulation via CCL2

“…Oviducts were collected from cows at a local slaughterhouse within 10–20 min of exsanguination. The stage of the estrous cycle was determined based on macroscopic observations of the ovary and uterus, where healthy oviducts were selected 22 . Oviductal tissues ipsilateral to the corpus luteum were collected at days 1–3 after ovulation (which represents the essential phases of fertilization and early embryo development within the oviduct), transported to the laboratory (4°C, in phosphate‐buffered saline (PBS) containing 10 µg/ml gentamicin (Sigma‐Aldrich) and 250 µg/ml amphotericin B (Sigma‐Aldrich)).…”