Accumulation of Cyanidin-3-glucoside in Callus and Cell Cultures of Oxalis reclinata

Crouch, Neil R.; Staden, L. Frances van; Staden, J. Van; Drewes, F. E.; Drewes, Siegfried E.; Meyer, H.J.

doi:10.1016/s0176-1617(11)80116-1

Cited by 15 publications

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References 12 publications

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“…Recently cyanidin-3-glucoside was isolated from in vitro cultured Oxalis linearis Jacq. callus (Crouch et al 1993). This paper deals with the effect of growth regulators, and nutrients on the growth of callus and the yield of anthocyanin produced in vitro by selected callus cultures of Oxalis linearis.…”

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The in vitro production of an anthocyanin from callus cultures of Oxalis linearis

Meyer

Staden

1995

Plant Cell Tiss Organ Cult

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A b s t r a c tCallus growth and the production of anthocyanins were sustained on the salts and vitamins of Murashige and Skoog. Callus growth was stimulated at a concentration of 8-32 gM ct-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D). Benzyladenine (BA) and zeatin at 8 gM inhibited callus growth whereas isopentenyladenine (iP) stimulated callus growth. NAA repressed anthocyanin production with an increase in NAA from 8-32 gM. Anthocyanin synthesis was promoted by an increase in 2,4-D from 0.5 to 2 gM and decreased thereafter up to a concentration 32 gM 2,4-D. A concentration of 8 gM BA, thidiazuron and zeatin, respectively stimulated pigment production. Sucrose stimulated callus growth at 60 mM and pigment production at 120-360 mM.Abbreviations: BA -6-benzyladenine, 2,4-D -2,4-dichlorophenoxyacetic acid, NAA -c~-naphthaleneacetic acid, iP -isopentenyladenine, TZ -thidiazuron -N-phenyl-N'-1,2,3-thiadiazol-5-yl-urea, Bu-HC1 -Butanol-2N HC1, BAW -Butanol-acetic acid-water I n t r o d u c t i o nAnthocyanins are currently studied anew as a source of colorants due to public pressure against the use of synthetic dyes. The small biomass produced by members of the Oxalidaceae does not make it economically feasible to produce pigments on a large scale from plants. Recently cyanidin-3-glucoside was isolated from in vitro cultured Oxalis linearis Jacq. callus (Crouch et al. 1993). This paper deals with the effect of growth regulators, and nutrients on the growth of callus and the yield of anthocyanin produced in vitro by selected callus cultures of Oxalis linearis. M a t e r i a l s a n d m e t h o d sThe basal medium consisted of MS salts and vitamins (Murashige & Skoog 1962) supplemented with 60 mM sucrose and solidified with 0.2% Gelrite. The pH of the medium was adjusted to 5.7 before autoclaving for 20 min at 121°C. Shoots obtained from mature Oxalis linearis plants were surface sterilised for 10 min in 0.2% HgC12 followed by five rinses in sterile distilled water. One cm long explants were placed on a shoot initiation medium in glass tubes (24 mm ~b × 100 mm long) and sealed with parafilm. The shoot initiation medium consisted of the basal medium supplemented with 25 gM BA and 1 gM NAA. Red pigmented callus was isolated and transferred onto the basal medium containing 8 gM NAA and incubated in the dark to produce non-pigmented callus stock. The effect of growth regulators on callus growth and anthocyanin production was determined by subjecting dark grown callus to 0-32 gM NAA in combination with 0 and 8 gM BA as well as NAA (8 gM) containing medium with 8 gM BA, TZ, iP, kinetin or zeatin. To determine whether 2,4-D repressed anthocyanin production dark grown callus was incubated on 0.5-32 gM 2,4-D. The optimal sucrose concentration for anthocyanin production was

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The in vitro production of an anthocyanin from callus cultures of Oxalis linearis

Meyer

Staden

1995

Plant Cell Tiss Organ Cult

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“…Cell cultures may accumulate different secondary metabolites from those found in vivo, or may produce insignificant quantities of some components (Nawa et al, 1993;Mori et al, 1993;Wilson, 1990). In other cases, product yields and/or quality traits may be enhanced through production by tissue culture (Cormier and Do, 1993;Crouch et al, 1993). Manipulation of the physical and chemical microenvironments can influence both the quality and yield of natural products (Callebaut et al, 1990;Do and Cormier, 1991).…”

Section: Introduction the Characteristicmentioning

confidence: 99%

Expression of Anthocyanins in Callus Cultures of Cranberry (Vaccinium macrocarpon Ait)

Madhavi

Smith

Berber-Jimenez

1995

Journal of Food Science

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Expression of anthocyanins and other flavonoids in callus cultures established from different parts of cranberry plant was investigated and the effect of explant source on the in vitro product was determined. Callus cultures were initiated from different parts of the plant in a modified Gamborg's medium with 5.37 pM o-naphthaleneacetic acid, 0.45 pM 2,4-dichlorophenoxyacetic acid, and 2.32 pM kinetin in the dark at 25°C. Callus cultures accumulated anthocyanins only on exposure to light and maximum concentration was observed by day 12. The cultures had lower levels of anthocyanins and only cyanidin 3-galactoside, cyanidin 3-glucoside, and cyanidin 3-arabinoside were identified in all cultures regardless of source of explant. Proanthocyanidin accumulation in cultures was independent of light, and levels were higher than in mature fruit. Exposure to light induced accumulation of flavonols and enhanced activity of phenylalanine ammonia-lyase in the cultures.

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“…In callus cells, synthesis of photosynthetic pigments depends on many factors: part of plant from which explants are obtained (Nakashima and Tsuda 1987), conditions of callus subculturing (Gaspar et al 1988), mainly substrate characteristics Ohtani 1992, Crouch et al 1993), and plant species (Krsti6 et al 1992). The rate of pigment synthesis depends primarily on the rate of precursor synthesis (Nawa et al 1993, Bisbis et al 1994) and of chloroplast forming (Crevecoeur et al 1992, Lavergne et al 1992. The aim of our work was to determine the Chl and Car concentrations in calli of sugar beet genotypes.…”

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Chlorophylls and carotenoids in calli of sugar beet genotypes

Krstic¹,

Mezei²,

Kovačev³

et al. 1996

Biol. plant.

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Chlorophyll (Chl) and carotenoid (Car) contents in calli (hypocotyl, cotyledon, egg cell) showed statistically significant differences between sugar beet genotypes. Absorption spectra of extracts from green calli did not differ from those obtained from green leaves, while in "brown" calli the differences were caused by relatively high Car contents. Pigment contents in calli were significantly lower than in leaves.

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Accumulation of Cyanidin-3-glucoside in Callus and Cell Cultures of Oxalis reclinata

Cited by 15 publications

References 12 publications

The in vitro production of an anthocyanin from callus cultures of Oxalis linearis

The in vitro production of an anthocyanin from callus cultures of Oxalis linearis

Expression of Anthocyanins in Callus Cultures of Cranberry (Vaccinium macrocarpon Ait)

Chlorophylls and carotenoids in calli of sugar beet genotypes

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