Accumulation of coumarins in Arabidopsis thaliana

Kai, Kosuke; Shimizu, Bun-ichi; Mizutani, Masaharu; Watanabe, Ken; Sakata, Kanzo

doi:10.1016/j.phytochem.2005.11.006

Cited by 150 publications

(132 citation statements)

References 28 publications

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“…This is the first report on the occurrence of lariciresinol in A. thaliana. GC-MS analysis indicated that the lignan was localized in roots like coumarins (11), whereas other typical lignans, such as pinoresinol, secoisolariciresinol, or matairesinol, were detected neither in the roots nor in the aerial parts.…”

Section: Discussionmentioning

confidence: 95%

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Characterization of Arabidopsis thaliana Pinoresinol Reductase, a New Type of Enzyme Involved in Lignan Biosynthesis

Nakatsubo

Mizutani

Suzuki

et al. 2008

Journal of Biological Chemistry

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103

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A lignan, lariciresinol, was isolated from Arabidopsis thaliana, the most widely used model plant in plant bioscience sectors, for the first time. In the A. thaliana genome database, there are two genes (At1g32100 and At4g13660) that are annotated as pinoresinol/lariciresinol reductase (PLR). The recombinant AtPLRs showed strict substrate preference toward pinoresinol but only weak or no activity toward lariciresinol, which is in sharp contrast to conventional PLRs of other plants that can reduce both pinoresinol and lariciresinol efficiently to lariciresinol and secoisolariciresinol, respectively. Therefore, we renamed AtPLRs as A. thaliana pinoresinol reductases (AtPrRs).

show abstract

Section: Discussionmentioning

confidence: 95%

“…Preliminary GC-MS analysis of MeOH extracts from whole plants of A. thaliana showed the presence of trace amounts of lariciresinol (data not shown). However, recently, Kai et al (11) found that coumarins, which belong to another class of phenylpropanoid compounds, were significantly accumulated in the plant roots.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Arabidopsis thaliana Pinoresinol Reductase, a New Type of Enzyme Involved in Lignan Biosynthesis

Nakatsubo

Mizutani

Suzuki

et al. 2008

Journal of Biological Chemistry

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103

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“…These data further support the hypothesis of Chandran et al (2003) that Suc transporters function in the transport of glucosides in addition to Suc. Coumarins, such as esculin, have recently been found in small quantities in Arabidopsis (Kai et al, 2006), and they have been shown to function in plant defense against herbivorous insects (Silva et al, 2006). Because AtSUC9 transports salicin and arbutin with very high affinities (Fig.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant Phenotype

et al. 2006

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(C.P.L.G., J.M.P.) AtSUC9 (At5g06170), a sucrose (Suc) transporter from Arabidopsis (Arabidopsis thaliana) L. Heynh., was expressed in Xenopus (Xenopus laevis) oocytes, and transport activity was analyzed. Compared to all other Suc transporters, AtSUC9 had an ultrahigh affinity for Suc (K 0.5 5 0.066 6 0.025 mM). AtSUC9 showed low substrate specificity, similar to AtSUC2 (At1g22710), and transported a wide range of glucosides, including helicin, salicin, arbutin, maltose, fraxin, esculin, turanose, and a-methyl-Dglucose. The ability of AtSUC9 to transport 10 glucosides was compared directly with that of AtSUC2, HvSUT1 (from barley [Hordeum vulgare]), and ShSUT1 (from sugarcane [Saccharum hybrid]), and results indicate that type I and type II Suc transporters have different substrate specificities. AtSUC9 protein was localized to the plasma membrane by transient expression in onion (Allium cepa) epidermis. Using a whole-gene translational fusion to b-glucuronidase, AtSUC9 expression was found in sink tissues throughout the shoots and in flowers. AtSUC9 expression in Arabidopsis was dependent on intragenic sequence, and this was found to also be true for AtSUC1 (At1g71880) but not AtSUC2. Plants containing mutations in Suc transporter gene AtSUC9 were found to have an early flowering phenotype under short-day conditions. The transport properties of AtSUC9 indicate that it is uniquely suited to provide cellular uptake of Suc at very low extracellular Suc concentrations. The mutant phenotype of atsuc9 alleles indicates that AtSUC9 activity leads to a delay in floral transition.

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“…Fe Deficiency-Induced Synthesis and Secretion of Fluorescent Phenolics Is Dependent on F69H1 and FIT F69H1 utilizes molecular oxygen to catalyze the orthohydroxylation of feruloyl-CoA, which is a prerequisite for its spontaneous isomerization and lactonization in the formation of the coumarins scopolin, scopoletin, and hydroxy-feruloyl-CoA (Kai et al, 2006(Kai et al, , 2008. When exposed to UV light, coumarins characteristically exhibit blue fluorescence.…”

Section: Isolation Of a T-dna Insertion Line With Strong Chlorosis Unmentioning

confidence: 99%

Feruloyl-CoA 6'-Hydroxylase1-Dependent Coumarins Mediate Iron Acquisition from Alkaline Substrates in Arabidopsis

Schmid¹,

Giehl²,

Döll³

et al. 2013

PLANT PHYSIOLOGY

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383

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Accumulation of coumarins in Arabidopsis thaliana

Cited by 150 publications

References 28 publications

Characterization of Arabidopsis thaliana Pinoresinol Reductase, a New Type of Enzyme Involved in Lignan Biosynthesis

Characterization of Arabidopsis thaliana Pinoresinol Reductase, a New Type of Enzyme Involved in Lignan Biosynthesis

Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant Phenotype

Feruloyl-CoA 6'-Hydroxylase1-Dependent Coumarins Mediate Iron Acquisition from Alkaline Substrates in Arabidopsis

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