Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats

Hu, Gao-Sheng; Huang, Chonggui; Zhang, Yang; Xiao, Wei; Jia, Jing‐Ming

doi:10.1016/s1875-5364(17)30043-2

Cited by 12 publications

(12 citation statements)

References 20 publications

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“…[5,6,7]. Modern studies have shown that triterpenoids in W. cocos have diverse pharmacological effects, such as anti-epilepsy, anti-apoptosis, diuresis, cytotoxicity, liver protection, anti-leukemia, anti-inflammatory, and so on [8,9,10,11,12,13,14,15,16]. Therefore, W. cocos has a wide and important role in future medical application.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Characteristics of Triterpenoid Transcriptome from Different Strains of Wolfiporia cocos

GuiPing

Zhao

2019

IJMS

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The dried sclerotia of Wolfiporia cocos (Schwein.) Ryvarden & Gilb., a traditional Chinese medicine, has triterpenoid as its main active component. Breeding high-yield triterpenoid in W. cocos is an important research topic at present. We screened out two monosporal strains from the same W. cocos 5.78, high-yielding DZAC-Wp-H-29 (H) and low-yielding DZAC-Wp-L-123 (L), and cultured mycelia for 17 days, 34 days, and 51 days, respectively. Transcriptome analysis results showed that triterpenoid synthesis is closely related to gene expression in triterpenoid synthesis pathways (hydroxymethyl glutaryl-CoA reductase (HMGCR), farnesyl diphosphate synthase (FDPS), 4-hydroxybenzoate polyprenyltransferase (COQ2), C-8 sterol isomerase (ERG2), sterol O-acyltransferase (ACAT), tyrosine aminotransferase (TAT), torulene dioxygenase (CAO2), and sterol-4alpha-carboxylate 3-dehydrogenase (erg26)), and is limited by the expression of enzyme M20 combined with domain protein peptide (Pm20d2), aryl-alcohol dehydrogenase (norA), ISWI chromatin-remodeling complex ATPase ISW2, GroES-like protein (adh), cytochrome P450 (ftmP450-1), and unknown proteins unigene0001029 and unigene0011374. In addition, maintaining high triterpenoid accumulation in W. cocos may require a stable membrane structure, so the accumulation ability may be related to the high synthesis ability of sterols. The low accumulation of triterpenoid in W. cocos may be due to the products of key enzymes increasing flow to other pathways.

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Section: Introductionmentioning

confidence: 99%

Comparative Analysis of the Characteristics of Triterpenoid Transcriptome from Different Strains of Wolfiporia cocos

GuiPing

Zhao

2019

IJMS

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“…It is worth noting that the liquid fermentation of mycelia could be an alternative to producing P. cocos , just like the fermentation of Cordyceps fungus and Ganoderma [11,12]. The accumulation of the mycelia biomass of P. cocos through fermentation has been demonstrated to be possible [13,14]. However, to date, there are few reports on the comparison of the bioactive triterpene acids profile in natural sclerotia and in fermented mycelia of P. cocos .…”

Section: Introductionmentioning

confidence: 99%

Insights into Triterpene Acids in Fermented Mycelia of Edible Fungus Poria cocos by a Comparative Study

et al. 2019

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As an edible sclerotia-forming fungus, Poria cocos is widely used as a food supplement and as a tonic in China. High-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) was applied to identify triterpene acids in fermented mycelia of P. cocos, as well as the epidermis and inner part of natural sclerotia. A total of 19 triterpene acids were identified in fermented mycelia, whereas 31 were identified in the epidermis and 24 in the inner part. Nine triterpene acids were quantitatively determined, and the concentrations of two valuable triterpenes, dehydropachymic acid and pachymic acid, reached 1.07 mg/g and 0.61 mg/g in the fermented mycelia part, respectively, and were both significantly higher than the concentration in the two natural parts. The fermented mycelia could be a good choice for producing some target triterpene compounds and functional foods through fermentation thanks to the high concentration of some triterpene acids.

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“…These results demonstrated the advantages for producing useful secondary metabolites using the fermentation of P. cocos mycelia. Our previously published result proved that a higher content of triterpenoids in a two-stage cultured mycelia of P. cocos had stronger diuretic activity in rats compared with cultivated sclerotium [28].…”

Section: Triterpenoid Content Determinationmentioning

confidence: 84%

Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos

Wang

Huang²,

Zhao

et al. 2020

Molecules

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Poria cocos, an important medicinal and edible fungus, is well known in East Asia. The main active components are water-soluble polysaccharides (WPS) and triterpenoids. Due to the growing market demand, long cultivation period, and consumption of pine trunk during cultivation, alternative methods for producing P. cocos or its active components should be investigated. In this study, WPS, triterpenoids, monosaccharide composition, and essential oil in fermented mycelia and cultivated sclerotium were analyzed using UV spectrophotometry, HPLC, pre-column derivatization, and HS-GC/MS, respectively. Our results showed that the WPS and triterpenoids in mycelia are several times higher than those in sclerotium. Among the 62 compounds identified by HS-GC/MS analysis from the essential oil obtained from the fermentation media and a fresh external layer, the two main fragrances in common were linalool and methyl phenylacetate. Our results suggested that it is applicable to produce polysaccharides and triterpenoids by the fermentation of P. cocos, and a strategy to improve triterpenoid production in the fermentation process was proposed.Molecules 2020, 25, 1269 2 of 12 by 11.5-18.5% and enhance the antioxidant enzyme activity when fed for 7 weeks at the daily dose of 100-200 mg/Kg in liver cancae male Wistar rats [12]. Recently, APS was reported to improve hyperglycemia, hyperlipidemia, and hepatic steatosis in ob/ob mice via the modulation of gut microbiota [13]. Exopolysaccharides (EPS), another kind of water-soluble polysaccharide, is produced in the media during the liquid fermentation of P. cocos, and it has also been reported to have anti-tumor activity both in vivo (Sarcoma-180 solid tumors implanted in BALB/c mice, a immune deficient inbred mice line) and in vitro (HL-60 tumor cells) [6] as well as antioxidant activities [14]. Triterpenoids are reported to be responsible for the diuretic [15], anti-tumor [16], and anti-inflammatory activities [8] of P. cocos. More than 50 triterpenoids have been purified from P. cocos, derived from lanostane or secolanostane skeletons [17]. A more systematic introduction about the chemistry and pharmacology activity of P.cocos has been reviewed [17]. The main constituents are dehydrotumulosic acid (DTA), 3-epi-dehydrotumulosic acid (eDTA), and polyporenic acid C (PAC) [18].As a consequence of its nutritional and health values, P. cocos has gained wide popularity as a nutraceutical and functional food in China. Normally, P. cocos grows underground around the roots or dried trunks of pine trees and needs a long cultivation period of about 7 months. In recent decades, the fermentation culture of P. cocos has been developed because of the potential for the increased production of mycelia and bioactive components in a compact space and in a shorter period of time. Many studies have been conducted to examine the influence of different conditions on P. cocos culture, including medium composition, temperature, and environmental conditions [19][20][21]. The liquid culture of P. cocos has been...

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Accumulation of biomass and four triterpenoids in two-stage cultured Poria cocos mycelia and diuretic activity in rats

Cited by 12 publications

References 20 publications

Comparative Analysis of the Characteristics of Triterpenoid Transcriptome from Different Strains of Wolfiporia cocos

Comparative Analysis of the Characteristics of Triterpenoid Transcriptome from Different Strains of Wolfiporia cocos

Insights into Triterpene Acids in Fermented Mycelia of Edible Fungus Poria cocos by a Comparative Study

Comparative Studies on Polysaccharides, Triterpenoids, and Essential Oil from Fermented Mycelia and Cultivated Sclerotium of a Medicinal and Edible Mushroom, Poria Cocos

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