Accumulation of altered viral nucleocapsids in mumps virus?Persistently infected cell cultures

Og, Andzhaparidze; YuS, Boriskin; Nn, Bogomolova; Vd, Lotte

doi:10.1007/bf01314894

Cited by 6 publications

(4 citation statements)

References 13 publications

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“…The establishment of persistent infection in several cultured cell lines by paramyxoviridae, including measles and mumps viruses, has been reported (Andzhaparidze et al, 1983 ;Barry et al, 1976;Knight et al, 1972;Menna et al, 1975 ;Truant & Hallum, 1977). Persistent infection of measles virus in the human central nervous system is closely associated with subacute sclerosing panencephalitis (SSPE) (Carter et al, 1983;Hall & Choppin, 1981;Wechsler & Meissner, 1982).…”

Section: Discussionmentioning

confidence: 99%

Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

Fujii¹,

Oguma²,

Kimura³

et al. 1988

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

Fujii¹,

Oguma²,

Kimura³

et al. 1988

Journal of General Virology

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“…Some cells were hypertrophied (area 4280+367 ~t 2) and contained 3-5 nuclei; cell fusion with formation of small clusters was also observed. In contrast to L-41, HEp-2, and other cell systems [1,2], human astrocytes did not form giant symplasts, but their shape changed from stellate to spindle-like. Virusspecific antigens were revealed by indirect immunofluorescence in 56.5+4.46% of cells 24-48 h after infection.…”

Section: Resultsmentioning

confidence: 53%

“…Replicating in monocytes/macrophages, this virus induces antigenic modulations consisting in the emergence of virusinduced proteins in the plasma membrane [2,5,6]. This phenomenon manifests itself as the ability of infected leukocytes or bone marrow cells to adsorb swine erythrocytes with serotype-specific abrogation of delayed hemadsorption (DHA) [1,9]. Hemadsorption is believed to be mediated by a virus-specific protein which is functionally and structurally similar Laboratory of Biochemistry, Institute of Veterinary Virology and Microbiology, Pokrov to the CD2 surface antigen of T lymphocytes [7].…”

Section: Cell-to-cell Interactions In Cultures Ofmentioning

confidence: 98%

Infection of highly differentiated human and guinea pig astrocytes with herpes simplex and measles viruses

Poleshchuk¹,

Kvacheva²,

Ul'kevich³

et al. 1996

Bull Exp Biol Med

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The morphogenesis of type I herpes simplex virus and measles virus (Edmonston strain) is studied in primary cultures of highly differentiated human and guinea pig astrocytes. It is shown that astrocytes are involved in infection. Under varied conditions of inoculation, herpes simplex virus causes acute infection, while measles virus induces both acute and chronic infection. Chronic infection is associated with impaired assembly of virions, accumulation of thickened ribonucleoproteins (25-37 nm in diameter) in the cytoplasm, and budding of "empty" viral particles into the extracellular space. Persisting measles virus reactivates 78_+9.4% of cells, which is accompanied by hypertrophy and hyperproduction of the glial fibrillar acid protein. Key Words: primary cultures; human and guinea pig astrocytes; herpes simplex virus; measles virusRecent neuroimmunological studies show that antigen-activated astrocytes are capable of phagocytosis, production of tumor necrosis factor, intefleukins-la, -113, -6, and aj-interferon, and expression of I and II class proteins of the major histocompatibility complex, which led to reconsideration of the role of astrocytes in the pathogenesis of encephalomyelitis and slow degenerative processes in the central nervous system (CNS) [4,6,8,9]. However, evaluation of the significance of astroglia in the maintenance-of infectious process in the CNS and in its chronization was hampered for a long time by the absence of adequate in vitro models.In the present study we compared the sensitivity of primary cultures of highly differentiated human and guinea pig astrocytes to measles virus (MV) and Byelorussian Institute of Epidemiology and Microbiology, Byelorussian Ministry of Health, Minsk; Minsk State Medical Institute type I herpes simplex virus (HSV-1) with a parallel investigation of morphology, cytoarchitectonics, and expression of the glial fibrillar acid protein (GFAP) by these cells. MATERIALS AND METHODSPrimary confluent cultures of human and guinea pig astrocytes were obtained as described elsewhere [5]. Astrocytes were identified by indirect immunofluorescence using anti-GFAP antiserum (Serva) and specific ultrastructural characteristics [3]. Measles virus (strain Edmonston) and HSV-1 were added to lst-2nd passage cultures in dose ranges 0.01-0.1 and 1-10 TCDJml during logarithmic growth. Virus accumulation was assessed by adding 10-fold serial dilutions of growth medium (from lif t to 10 .9 ) and cell lysates to test-systems: chick embryo fibroblasts for HSV-1 and L-41 for MV. The expression of viral

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“…However, the patient's serum tested positive for anti-NiV IgG, although the specific protein targeted by the IgG response could not be determined. It is possible that the patient is persistently infected with an NiV that has altered viral nucleocapsids, resulting in defective or non-infectious virus, as has been previously observed in other paramyxovirus infections, including mumps (27) and measles (28). Thus, it is possible that the anti-N antibody produced against the mutated virus only weakly recognises rNiV-N in comparison to those in the acute positive patients' sera.…”

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confidence: 94%

Serum from Nipah Virus Patients Recognises Recombinant Viral Proteins Produced in <i>Escherichia coli</i>

et al. 2017

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SUMMARY:The genes for Nipah virus (NiV) proteins were amplified from viral RNA, cloned into the plasmid pTriEx-3 Hygro, expressed, and purified using immobilized metal affinity chromatography. The recombinant N, F, and G NiV proteins (rNiV-N, rNiV-F, and rNiV-G), were successfully expressed in Escherichia coli and purified with a yield of 4, 16, and 4 mg/L, respectively. All 3 recombinant viral proteins reacted with all 19 samples of NiV-positive human sera. The rNiV-N and rNiV-G proteins were the most immunogenic. The recombinant viral proteins did not react with any of the 12 NiV-negative sera. However, serum from a patient with a late-onset relapsing NiV infection complication was found to be primarily reactive to rNiV-G only. Additionally, there is a distinctive variation in the profile of antigen-reactive bands between the sample from a case of relapsing NiV encephalitis and that of acute NiV infection. The overall findings of this study suggest that the recombinant viral proteins have the potential to be developed further for use in the detection of NiV infection, and continuous biosurveillance of NiV infection in resource-limited settings.

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Accumulation of altered viral nucleocapsids in mumps virus?Persistently infected cell cultures

Cited by 6 publications

References 13 publications

Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

Oligo-2',5'-adenylate Synthetase Activity in K562 Cell Lines Persistently Infected with Measles or Mumps Virus

Infection of highly differentiated human and guinea pig astrocytes with herpes simplex and measles viruses

Serum from Nipah Virus Patients Recognises Recombinant Viral Proteins Produced in <i>Escherichia coli</i>

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