Accumulation of 8-nitroguanine in human gastric epithelium induced by Helicobacter pylori infection

8-Nitroguanine, a Potential Biomarker to Evaluate the Risk of Inflammation-Related Carcinogenesis

“…Thus, 8-nitroguanine is a potentially mutagenic DNA lesion, which can participate in initiation and promotion in the infection-related carcinogenesis (Loeb & Preston, 1986;. Our studies have demonstrated that 8-nitroguanine is formed at the sites of carcinogenesis in humans and experimental animals (Ma et al, 2004;Pinlaor et al, 2004b;Ding et al, 2005;Horiike et al, 2005;Ma et al, 2006;Hoki et al, 2007a;Hoki et al, 2007b;Fujita et al, 2008;Ma et al, 2008;Tanaka et al, 2008;Ma et al, 2009;Ma et al, 2010). Moreover, our studies have demonstrated that 8-nitroguanine was formed in Oct3/4-positve stem cells in S. haematobium-associated cystitis and cancer tissues.…”

“…We have firstly demonstrated that 8-nitroguanine is formed at the sites of carcinogenesis regardless of etiology, and we have proposed the possibility that 8-nitroguanine is a potential biomarker to evaluate the risk of inflammation-associated carcinogenesis . In clinical specimens, 8-nitroguanine was formed in the gastric grand epithelial cells of patients with gastritis caused by Helicobacter pylori (H. pylori) infection (Ma et al, 2004), hepatocytes of patients with chronic hepatitis C (Horiike et al, 2005), oral precancerous lesions oral lichen planus (OLP) (Chaiyarit et al, 2005) and oral leukoplakia , soft tissue sarcoma (Hoki et al, 2007a;Hoki et al, 2007b) and Epstein-Barr virus (EBV)-associated nasopharyngeal carcinoma (NPC) .…”

“…It is noteworthy that DNA damage was specifically induced at sites of carcinogenesis under various inflammatory conditions. In human samples, 8-nitroguanine formation was observed in gastric gland epithelial cells of patients with H. pylori infection (Ma et al, 2004) and in hepatocytes of patients with chronic hepatitis C (Horiike et al, 2005). 8-Nitroguanine was also formed in oral epithelium of OLP and OSCC patients (Chaiyarit et al, 2005;.…”

Role of Chronic Inflammation and Resulting DNA Damage in Cervical Carcinogenesis Induced by Human Papillomavirus

“…Administration of the antiparasitic drug praziquantel and the antioxidant curcumin significantly reduced oxidative and nitrative DNA damage Prakobwong et al, 2011). We have also demonstrated that 8-nitroguanine was formed in the gastric gland epithelial cells from gastritis patients with Helicobacter pylori infection (Ma et al, 2004), in the hepatocytes of patients with chronic hepatitis C (Horiike et al, 2005) and in the nasopharyngeal epithelial cells and tumor tissues of patients infected with Epstein-Barr virus (EBV) (Ma et al, 2008). These results suggest that 8-nitroguanine is a promising biomarker providing an assessment of the risk of inflammation-mediated carcinogenesis at the precancerous stage.…”

Oxidative Stress Pathway Driven by Inflammation in Gastric Mucosa

“…A double immunofluorescence labeling study demonstrated that the level of 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) apparent in gastric gland epithelium was significantly higher in gastritis patients with H. pylori infection than in those without infection. This results suggest that 8-nitroguanine could be a promising biomarker of inflammation (Ma, 2004). Kawanishi et al examined the formation of 8-nitroguanine, a nitrative DNA lesion, in humans and animals under inflammatory conditions.…”