Accumulation mechanisms and subcellular distribution of Cu in maize grown on soil treated with [S, S]-ethylenediamine disuccinic acid

Niu, Liyuan; Shen, Zhenguo; Luo, Chunling; Deng, Yaomin; Wang, Chunchun

doi:10.1007/s11104-011-0954-0

Cited by 11 publications

(11 citation statements)

References 38 publications

(53 reference statements)

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“…Moreover, EDDS is slightly toxic to plant roots (Fässler et al 2010). Upon EDDS application, plants may have suffered from the co-toxicity of Cu 2+ , EDDS, and the Cu-EDDS complex; the phytotoxicity has been demonstrated to be in the order Cu 2+ > EDDS > Cu-EDDS (Niu et al 2012). In the present study, the growth of both shoots and roots was significantly affected by the application of EDDS.…”

Section: Corn Growth and Cu Uptake Influenced By Eddsmentioning

confidence: 99%

Simultaneous enhanced removal of Cu, PCBs, and PBDEs by corn from e-waste-contaminated soil using the biodegradable chelant EDDS

Wang

Lei

et al. 2015

Environ Sci Pollut Res

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We evaluated the influence of the biodegradable chelant ethylenediamine disuccinic acid (EDDS) on plant uptake of polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and Cu by corn from electronic waste (e-waste)-contaminated soil. The highest concentration and highest total uptake of Cu in corn were observed in the treatment with 5 mM EDDS, which resulted in a 4-fold increase of the Cu translocation factor (C(shoot)/C(root)) compared to the control. The concentrations of PCBs and PBDEs in shoots and roots increased with increasing application rates of EDDS, and 1.58- and 1.32-fold average increases in the concentrations of PCBs and PBDEs, respectively, were observed in shoots in the EDDS treatments. A significant positive correlation was observed between shoot Cu and shoot PCBs and PBDEs. We speculate that PCBs and PBDEs were activated by the EDDS-triggered dissolved organic carbon (DOC) and then indiscriminately taken up by roots and translocated to shoots following damage to the roots mainly by the increased extractable Cu resulting from the EDDS application.

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Section: Corn Growth and Cu Uptake Influenced By Eddsmentioning

confidence: 99%

Simultaneous enhanced removal of Cu, PCBs, and PBDEs by corn from e-waste-contaminated soil using the biodegradable chelant EDDS

Wang

Lei

et al. 2015

Environ Sci Pollut Res

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“…Some studies have indeed shown direct uptake of the complex, but at large (mM) concentration of complexes, mostly in phytoremediation studies. For instance, Niu et al (2012) found that copper (Cu) in plants grown on soil treated with [S, S]-ethylenediamine disuccinic acid (EDDS; lowest concentration 0.5 mmol/kg) was mainly as Cu-EDDS. At all concentrations, plant yield was significantly reduced, suggesting damage to the plants.…”

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confidence: 99%

Diffusion Limitations in Root Uptake of Cadmium and Zinc, But Not Nickel, and Resulting Bias in the Michaelis Constant

et al. 2012

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It has long been recognized that diffusive boundary layers affect the determination of active transport parameters, but this has been largely overlooked in plant physiological research. We studied the short-term uptake of cadmium (Cd), zinc (Zn), and nickel (Ni) by spinach (Spinacia oleracea) and tomato (Lycopersicon esculentum) in solutions with or without metal complexes. At same free ion concentration, the presence of complexes, which enhance the diffusion flux, increased the uptake of Cd and Zn, whereas Ni uptake was unaffected. Competition effects of protons on Cd and Zn uptake were observed only at a very large degree of buffering, while competition of magnesium ions on Ni uptake was observed even in unbuffered solutions. These results strongly suggest that uptake of Cd and Zn is limited by diffusion of the free ion to the roots, except at very high degree of solution buffering, whereas Ni uptake is generally internalization limited. All results could be well described by a model that combined a diffusion equation with a competitive Michaelis-Menten equation. Direct uptake of the complex was estimated to be a major contribution only at millimolar concentrations of the complex or at very large ratios of complex to free ion concentration. The true K m for uptake of Cd 2+ and Zn 2+ was estimated at ,5 nM, three orders of magnitude smaller than the K m measured in unbuffered solutions. Published Michaelis constants for plant uptake of Cd and Zn likely strongly overestimate physiological ones and should not be interpreted as an indicator of transporter affinity.Internalization of metals by biota is traditionally described by Michaelis-Menten kinetics (Wilkinson and Buffle, 2004). The K m corresponds to the concentration in solution at which the uptake is one-half of the maximal uptake, F max . The Michaelis-Menten equation relates the uptake flux, F, to the free ion concentration at the site of uptake, [M] s :If diffusion of a metal across a diffusive boundary layer adjacent to the roots is the rate-limiting step for uptake, the concentration at the site of uptake will be lower than that in the bulk solution. As a result, diffusion limitations result in an overestimate of the K m , if the concentration at the root surface is assumed to be the same as in the bulk solution, as is usually done. This bias in K m has been discussed in detail by Winne (1973) and has, for instance, been demonstrated experimentally for uptake of Glc in rabbit jejunum (Thomson and Dietschy, 1980) and for uptake of several sugars, amino acids, and bile acids in rat ileum (Wilson and Dietschy, 1974).Models used to predict ion availability and toxicity of metals by plants usually rely on the assumption that uptake is controlled by the free metal ion activity and the activity of competing ions in the bulk solution. For instance, the biotic ligand model (BLM), originally developed to predict metal toxicity to aquatic organisms, assumes that toxicity of an ion is mitigated by the presence of competing ions that bind on the biotic ligand (Paqu...

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“…This contrasts with the findings presented previously [ 7 ], where chelating agents appeared to be less damaging alone than when applied with equimolar Cu. The related chelating agent ethylenediamine- N , N ′-disuccinic acid (EDDS) has also been found to significantly increase both membrane permeability and cytoplasmic Cu levels, resulting in higher Cu accumulation in shoot tissue [ 12 ]. Organic acids including citric acid have previously been found to increase plasma membrane permeability to a greater extent than inorganic acids [ 69 ].…”

Section: Resultsmentioning

confidence: 99%

“…Fine Pb deposits were observed in mitochondria, chloroplasts and within plasmodesmata [ 71 ]. In a recent study with Zea mays [ 12 ], the chelator EDDS was found to increase cell membrane permeability, with a concomitant increase in shoot concentrations. Although this was attributed to expansion of the apoplastic pathway through injured passage cells [ 72 ], rather than to increased solute entry into the symplast, it indicates that strong chelating agents can permeabilize plant cell membranes.…”

Section: Resultsmentioning

confidence: 99%

“…Chelated metals are transported more readily to shoot tissue than free metal ions [ 9 , 10 , 11 ], with higher root-to-shoot translocation coefficients that are not proportional to transpiration rates. In addition to influencing metal transport, chelating agents have been found to alter plant physiology, increasing membrane permeability [ 12 , 13 ] while reducing Cu-induced peroxidative damage [ 7 ]. Multiple pathways exist for root-to-shoot metal transport [ 14 ]: the extracellular apoplastic pathway; the intracellular symplastic pathway; and the transcellular pathway that involves multiple membrane transport steps.…”

Section: Introductionmentioning

confidence: 99%

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Increased Uptake of Chelated Copper Ions by Lolium perenne Attributed to Amplified Membrane and Endodermal Damage

Johnson

Singhal

2015

IJMS

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The contributions of mechanisms by which chelators influence metal translocation to plant shoot tissues are analyzed using a combination of numerical modelling and physical experiments. The model distinguishes between apoplastic and symplastic pathways of water and solute movement. It also includes the barrier effects of the endodermis and plasma membrane. Simulations are used to assess transport pathways for free and chelated metals, identifying mechanisms involved in chelate-enhanced phytoextraction. Hypothesized transport mechanisms and parameters specific to amendment treatments are estimated, with simulated results compared to experimental data. Parameter values for each amendment treatment are estimated based on literature and experimental values, and used for model calibration and simulation of amendment influences on solute transport pathways and mechanisms. Modeling indicates that chelation alters the pathways for Cu transport. For free ions, Cu transport to leaf tissue can be described using purely apoplastic or transcellular pathways. For strong chelators (ethylenediaminetetraacetic acid (EDTA) and diethylenetriaminepentaacetic acid (DTPA)), transport by the purely apoplastic pathway is insufficient to represent measured Cu transport to leaf tissue. Consistent with experimental observations, increased membrane permeability is required for simulating translocation in EDTA and DTPA treatments. Increasing the membrane permeability is key to enhancing phytoextraction efficiency.

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Accumulation mechanisms and subcellular distribution of Cu in maize grown on soil treated with [S, S]-ethylenediamine disuccinic acid

Cited by 11 publications

References 38 publications

Simultaneous enhanced removal of Cu, PCBs, and PBDEs by corn from e-waste-contaminated soil using the biodegradable chelant EDDS

Simultaneous enhanced removal of Cu, PCBs, and PBDEs by corn from e-waste-contaminated soil using the biodegradable chelant EDDS

Diffusion Limitations in Root Uptake of Cadmium and Zinc, But Not Nickel, and Resulting Bias in the Michaelis Constant

Increased Uptake of Chelated Copper Ions by Lolium perenne Attributed to Amplified Membrane and Endodermal Damage

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