Accumulation and trapping of hepatitis A virus particles by electrohydrodynamic flow and dielectrophoresis

Abstract

Hepatitis A virus particles (d = 27 nm) were successfully accumulated and trapped in a microfluidic system by means of a combination of electrohydrodynamic flow and dielectrophoretic forces. Electric fields were generated in a field cage consisting of eight microelectrodes. In addition, high medium conductance (0.3 S/m) resulted in sufficient Joule heating and the corresponding spatial variation of temperature, density, and permittivity to induce electrohydrodynamic flow in the vicinity of the field cage. Flow vortices transport particles toward the center of the field cage, where dielectrophoretic forces cause permanent entrapment and particle aggregation. Spatial distribution of temperature, density, and permittivity as well as resulting flow patterns were modeled numerically and are in good agreement with experimental results. This accumulation scheme might be applicable to sample concentration enhancement in biosensor applications.

Frequency sweep rate dependence on the dielectrophoretic response of polystyrene beads and red blood cells

“…Particles [3][4][5] include bioparticles (DNA, 6 virus, 7 and protein 8 ) as well as cells (blood cell types, 9,10 cancer, [11][12][13] stem cells, 14 and yeast 15 ). The advantages to coupling DEP with microfluidics are small sample size (on the order of microliters), rapid analysis (approximately minutes to achieve results), minimal sample preparation, and minimal waste production.…”

Dielectrophoretic manipulation of ribosomal RNA

“…[7][8][9] The technique has been used to characterize and manipulate a number of biological particles ranging from micrometer-size cells and bacteria to spores and viruses of nanometer dimensions. [10][11][12] The latest development of the technique and the scope of its applications have been recently compiled in two comprehensive reviews. 9,13 The work presented here demonstrates for the first time the DEP collection and repulsion of 16S and 23S subunits of E. coli rRNA using microelectrodes.…”

Enhancing the Performance of Surface-based Biosensors by AC Electrokinetic Effects - a Review

“…The numerical simulations revealed that with a quadrupolar microelectrode the capturing of the virus was achieved by both DEP for the short range capture and electrothermal fluid flow to overcome diffusion limitations. Others were also able to achieve virus capture at low ionic strengths (1-100 mS m -1 ) and higher particle concentrations (>10 6 particles mL -1 ) (Hughes et al, 1998;Hughes et al, 2001;Pethig et al, 1992;Grom et al, 2006;. The dielectrophoretic capture and detection of a food borne pathogen, Listeria monocytogenes, was accomplished with the aid of the heat shock protein 60 (Hsp60) immobilized on a sensor's surface (Koo et al, 2009).…”