Accumulation and retention of micellar β-carotene and lutein by Caco-2 human intestinal cells

Garrett, Dean; Failla, Mark L.; Sarama, Robert J; Craft, Neal E.

doi:10.1016/s0955-2863(99)00044-3

Cited by 61 publications

(54 citation statements)

References 35 publications

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“…Tetrahydrofuran (THF), a commonly used solvent for carotenoid delivery in cell culture, oxidizes readily in culture media, which may lead to instability of test compounds and result in cytotoxicity (Xu et al 1999;Williams et al 2000;Shahrzad et al 2002;Hurst et al 2004;O'Sullivan et al 2007). Therefore, attempts have been made to improve carotenoid delivery by using vehicles other than THF, including dimethyl sulfoxide, cyclodextrin, lipoproteins, micelles and beadlets (Martin et al 1996;Stivala et al 1996;Garrett et al 1999;Pfitzner et al 2000;Lancrajan et al 2001;Vertzoni et al 2006). However, the employed vehicles are in part cytotoxic, poorly soluble, crystallize in the culture medium or need to be frequently prepared freshly (Stivala et al 1996;Xu et al 1999;Lancrajan et al 2001;Da Violante et al 2002;Shahrzad et al 2002;O'Sullivan et al 2004;Palozza et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

et al. 2010

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The present investigation aimed to compare fetal calf serum (FCS) and Tween 40 with the commonly employed tetrahydrofuran (THF) with respect to cytotoxicity, stability of the solubilized carotenoids, and uptake and accumulation of the xanthophylls astaxanthin (AX) and canthaxanthin (CX) in cultured human liver cells (HepG2). Incubation of HepG2 cells for 24 h with THF (C1.25%) or FCS (C11.25%) with or without AX (C25 lmol/L) or CX (C25 lmol/L) did not affect cell viability. Tween 40 (0.25-1.25% in medium) reduced cell viability by 75-99%. The stabilities of AX and CX in cell-free RPMI 1640 medium for B24 h were higher when delivered with THF instead of FCS. The doseand time-dependent accumulations of AX and CX (1-10 lmol/L) in HepG2 cells were higher when carotenoids were delivered with FCS compared to THF. In conclusion, FCS and THF, but not Tween 40, were suitable solvent systems for the delivery of AX and CX to HepG2 cells. In our experiments FCS was superior with regard to the uptake and accumulation of both carotenoids.

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Section: Introductionmentioning

confidence: 99%

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

et al. 2010

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“…Lycopene uptake was higher for the optimised soup in Cell study 1, but not in Cell study 2. Cellular uptake of carotenoids has previously been shown to be linearly dependent on the amount of carotenoids added to the cells [17,23,24,34]. The higher uptake of β-carotene from the optimised soup may therefore have been due to the higher carotene concentration in the micellar fraction ( Table 2, Figures 3(a), (b)).…”

Section: Caco-2 Cell Uptakementioning

confidence: 91%

“…Both methods have been considered to be appropriate tools for estimation of bioavailability of carotenoids in different plant matrices [19,20], but recent studies suggest that the bioaccessibility values obtained from the supernatant and micellar phase can be significantly different, even when samples from the same plant food have been investigated [21,22]. Cell culture models have also been utilised as part of in vitro digestion models, in particular the Caco-2 cell culture model has been used to estimate the absorption of bioactive components [23,24]. Even if not all aspects of bioavailability can be simulated, in vitro models can be used as simple, inexpensive and reproducible methods to study digestive stability, micellarization, intestinal transport and metabolism of carotenoids, and may allow prediction of bioavailability of different food components.…”

Section: Introductionmentioning

confidence: 99%

Applicability of in Vitro Models in Predicting the in Vivo Bioavailability of Lycopene and β-Carotene from Differently Processed Soups

Alminger¹,

Svelander²,

Wellner³

et al. 2012

FNS

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Presently, there is no clear consensus on the best approach to estimate carotenoid bioavailability. The best alternative would be to use human studies, but they are labour-intensive and expensive and can only be used to investigate a limited number of samples. Hence, a number of in vitro models have been developed to study pre-absorptive processes and factors affecting bioavailability. The question is, however, how well the results obtained by the various methods correlate to each other and to the in vivo situation. In the present paper, we have compared in vivo data from two human studies on differently processed soups containing carrots, tomato and broccoli, with results obtained by in vitro characterisation of the same soups. In vitro bioaccessibility was estimated by a static in vitro digestion investigating matrix release and micellarization of carotenoids and by uptake studies in a human intestinal cell line (Caco-2). In vivo data was obtained from clinical studies measuring total plasma carotenoid concentrations in human subjects after 4 weeks daily consumption of the soups. Comparison of the in vitro and in vivo results indicate that the combination of a two-step in vitro digestion and Caco-2 cells seems to be a useful tool for estimation of β-carotene bioaccessibility and screening of factors governing the release of β-carotene from this type of food. For lycopene the in vitro and in vivo results were less consistent, suggesting that reliable prediction of lycopene bioavailability might be more problematic.

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“…Competitive absorption of carotenoid transport and tissue concentrations exists between hydrocarbon carotenoids and xanthophyll carotenoids (115)(116)(117). Bioavailability studies have suggested that interactions between different groups of carotenoids may affect each other's bioavailability, either during micellization or during competition for gastrointestinal uptake into enterocytes, for chylomicron incorporation, or for tissue release from lipoproteins (118)(119)(120). High-dose dietary supplementation with a single carotenoid may alter the assimilation of other carotenoids (121).…”

Section: Introductionmentioning

confidence: 99%

The Age-Related Eye Disease 2 Study: Micronutrients in the Treatment of Macular Degeneration

Gorusupudi

Nelson

Bernstein

2017

Advances in Nutrition

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Age-related macular degeneration (AMD) is one of the leading causes of vision loss in the elderly. With an increasingly aged population worldwide, the need for the prevention of AMD is rising. Multiple studies investigating AMD with the use of animal models and cell culture have identified oxidative stress-related retinal damage as an important contributing factor. In general, diet is an excellent source of the antioxidants, vitamins, and minerals necessary for healthy living; moreover, the general public is often receptive to recommendations made by physicians and health care workers regarding diet and supplements as a means of empowering themselves to avoid common and worrisome ailments such as AMD, which has made epidemiologists and clinicians enthusiastic about dietary intervention studies. A wide variety of nutrients, such as minerals, vitamins, v-3 (n-3) fatty acids, and various carotenoids, have been associated with reducing the risk of AMD. Initial results from the Age-Related Eye Disease Study (AREDS) indicated that supplementation with antioxidants (b-carotene and vitamins C and E) and zinc was associated with a reduced risk of AMD progression. The AREDS2 follow-up study, designed to improve upon the earlier formulation, tested the addition of lutein, zeaxanthin, and v-3 fatty acids. In this review, we examine the science behind the nutritional factors included in these interventional studies and the reasons for considering their inclusion to lower the rate of AMD progression. Adv Nutr 2017;8:40-53.

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Accumulation and retention of micellar β-carotene and lutein by Caco-2 human intestinal cells

Cited by 61 publications

References 35 publications

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

Applicability of <i>in Vitro</i> Models in Predicting the <i>in Vivo</i> Bioavailability of Lycopene and <i>β</i>-Carotene from Differently Processed Soups

The Age-Related Eye Disease 2 Study: Micronutrients in the Treatment of Macular Degeneration

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Accumulation and retention of micellar β-carotene and lutein by Caco-2 human intestinal cells

Cited by 61 publications

References 35 publications

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

Comparison of tetrahydrofuran, fetal calf serum, and Tween 40 for the delivery of astaxanthin and canthaxanthin to HepG2 cells

Applicability of &lt;i&gt;in Vitro&lt;/i&gt; Models in Predicting the &lt;i&gt;in Vivo&lt;/i&gt; Bioavailability of Lycopene and &lt;i&gt;β&lt;/i&gt;-Carotene from Differently Processed Soups

The Age-Related Eye Disease 2 Study: Micronutrients in the Treatment of Macular Degeneration

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Applicability of <i>in Vitro</i> Models in Predicting the <i>in Vivo</i> Bioavailability of Lycopene and <i>β</i>-Carotene from Differently Processed Soups