Accumulating evidences of callose priming by indole- 3- carboxylic acid in response to <i>Plectospharella cucumerina</i>

Pastor-Fernández, Julia; Pastor, Victoria; Mateu, Diego; Gamir, Jordi; Sánchez-Bel, Paloma; Flors, Vı́ctor

doi:10.1080/15592324.2019.1608107

Cited by 17 publications

(8 citation statements)

References 9 publications

(11 reference statements)

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“…All three selected secondary metabolites, indole‐3‐carboxylic acid, jasmonic acid, and lasiodiplodin were characterized in previous studies as phytotoxins. Indole‐3‐carboxylic acid is a compound that induces resistance against necrotrophic fungal infections in mature Arabidopsis plants by mediating accelerated callose accumulation (Gamir et al., 2012; Pastor‐Fernández et al., 2019). Jasmonic acid is a plant growth regulator, an agent involved in plant defence responses, and a key regulator associated with leaf senescence.…”

Section: Discussionmentioning

confidence: 99%

Impact of temperature variation on the phytotoxic secondary metabolite production by Lasiodiplodia theobromae

Aluthmuhandiram

Chethana

Zhang³

et al. 2021

Journal of Phytopathology

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Botryosphaeriaceae species are associated with grapevine trunk diseases. Lasiodiplodia theobromae plays a significant role as one of the most virulent grapevine pathogens in the world. In this study, we evaluate the effects of temperature on phytotoxin‐induced virulence in Lasiodiplodia theobromae. The fungal strain is grown in Czapek‐Dox liquid medium at four different temperatures (20°C, 25°C, 30°C and 35°C) for 14 days under dark conditions. The culture filtrates were screened for three selected phytotoxins, indole‐3‐carboxylic acid, jasmonic acid, and lasiodiplodin using UPLC MS/MS techniques. Phytotoxicity of the crude culture filtrates was tested on detached grapevine leaves. With UPLC MS/MS analysis, we identified that indole‐3‐carboxylic acid and jasmonic acid production varied with the increasing temperature, and did not detect any lasiodiplodin. At high temperatures, we observed a significant increase in the phytotoxicity of crude culture filtrates on the detached grapevine leaves. The most severe necrotic leaf lesions were observed when inoculated with crude culture filtrates extracted at 35°C.

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Section: Discussionmentioning

confidence: 99%

Impact of temperature variation on the phytotoxic secondary metabolite production by Lasiodiplodia theobromae

Aluthmuhandiram

Chethana

Zhang³

et al. 2021

Journal of Phytopathology

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“…The indirect effects of ABA on callose during PDMP-induced resistance need further studies. The intact indole-3-carboxylic acid (I3CA) biosynthetic pathway has been reported to be necessary for I3CA-induced resistance ( Pastor-Fernández et al , 2019 ). I3CA can induce resistance in Arabidopsis against the necrotrophic fungus Plectosphaerella cucumerina through priming callose accumulation ( Gamir et al , 2018 ; Pastor-Fernández et al , 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…The intact indole-3-carboxylic acid (I3CA) biosynthetic pathway has been reported to be necessary for I3CA-induced resistance ( Pastor-Fernández et al , 2019 ). I3CA can induce resistance in Arabidopsis against the necrotrophic fungus Plectosphaerella cucumerina through priming callose accumulation ( Gamir et al , 2018 ; Pastor-Fernández et al , 2019 ). In our study, we found that the ABA biosynthetic pathway is necessary for PDMP-induced resistance; however, the underlying mechanism needs to be studied further in depth.…”

Section: Discussionmentioning

confidence: 99%

Penicillium chrysogenum polypeptide extract protects tobacco plants from tobacco mosaic virus infection through modulation of ABA biosynthesis and callose priming

Liu

Jiao

et al. 2021

Journal of Experimental Botany

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The polypeptide extract of the dry mycelium of Penicillium chrysogenum (PDMP) can protect tobacco plants from tobacco mosaic virus (TMV), although the potential mechanism underlying PDMP-mediated TMV resistance remains unknown. In our study, we analyzed this potential mechanism via RNA sequencing (RNA-seq) and found that the ABA biosynthetic pathway and β-1,3-glucanase, a callose-degrading enzyme, might play an important role in the PDMP-induced priming of resistance to TMV. To test our hypothesis, we successfully generated a Nicotiana benthamiana ABA biosynthesis mutant and evaluated the role of the ABA pathway in PDMP-induced callose deposition during plasmodesmata resistance to TMV infection via aniline blue staining, quantitative real-time polymerase chain reaction, western blotting, and immunoelectron microscopy. Our results suggested that PDMP can induce callose priming to defend against TMV movement. PDMP inhibited TMV movement by increasing callose deposition around plasmodesmata, but this phenomenon did not occur in the ABA biosynthesis mutant; moreover, these effects of PDMP on callose deposition could be rescued by treatment with exogenous ABA. Our results suggested that callose deposition around plasmodesmata in wild-type plants is mainly responsible for the restriction of TMV movement during the PDMP-induced defensive response to TMV infection and that ABA biosynthesis apparently plays a crucial role in PDMP-induced callose priming for enhancing defense against TMV.

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“…Similarly, Pi treatment in Arabidopsis plants was found to foster callose accumulation during infection with the fungal pathogen P. cucumerina . The relevance of callose deposition in resistance to pathogen infection in Arabidopsis, including P. cucumerina infection, is well established (Luna et al ., 2011; Pastor-Fernández et al ., 2019; Lee et al ., 2020). Third, nla plants showed an increase SA and JA content under non-infection, and accumulated higher levels of JA under infection conditions.…”

Section: Discussionmentioning

confidence: 99%

NITROGEN LIMITATION ADAPTATION functions as a negative regulator of Arabidopsis immunity

Torregrosa

Bundó

Chiou

et al. 2021

Preprint

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Background: Phosphorus is an important macronutrient required for plant growth and development. It is absorbed through the roots in the form of inorganic phosphate (Pi). To cope with Pi limitation, plants have evolved an array of adaptive mechanisms to facilitate Pi acquisition and protect them from stress caused by Pi starvation. The NITROGEN LIMITATION ADAPTION (NLA) gene plays a key role in the regulation of phosphate starvation responses (PSR), its expression being regulated by the microRNA miR827. Stress caused by Pi limiting conditions might also affect the plant response to pathogen infection. However, cross-talk between phosphate signaling pathways and immune responses remains unclear. Results: In this study, we investigated whether NLA plays a role in Arabidopsis immunity. We show that loss-of-function of NLA and MIR827 overexpression causes an increase in phosphate (Pi) content which results in resistance to infection by the fungal pathogen Plectosphaerella cucumerina. The nla mutant plants accumulated callose in their leaves, a response that is also observed in wild-type plants that have been treated with high Pi. We also show that pathogen infection and treatment with fungal elicitors is accompanied by transcriptional activation of MIR827 and down-regulation of NLA. Upon pathogen challenge, nla plants exhibited higher levels of the phytoalexin camalexin compared to wild type plants. Camalexin level also increases in wild type plants treated with high Pi. Furthermore, the nla mutant plants accumulated salicylic acid (SA) and jasmonic acid (JA) in the absence of pathogen infection whose levels further increased upon pathogen. Conclusions: This study shows that NLA acts as a negative regulator of Arabidopsis immunity. Overaccumulation of Pi in nla plants positively affects resistance to infection by fungal pathogens. This piece of information reinforces the idea of signaling convergence between Pi and immune responses for the regulation of disease resistance in Arabidopsis.

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Accumulating evidences of callose priming by indole- 3- carboxylic acid in response to Plectospharella cucumerina

Cited by 17 publications

References 9 publications

Impact of temperature variation on the phytotoxic secondary metabolite production by Lasiodiplodia theobromae

Impact of temperature variation on the phytotoxic secondary metabolite production by Lasiodiplodia theobromae

Penicillium chrysogenum polypeptide extract protects tobacco plants from tobacco mosaic virus infection through modulation of ABA biosynthesis and callose priming

NITROGEN LIMITATION ADAPTATION functions as a negative regulator of Arabidopsis immunity

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