Impact of temperature variation on the phytotoxic secondary metabolite production by Lasiodiplodia theobromae

Abstract

Botryosphaeriaceae species are associated with grapevine trunk diseases. Lasiodiplodia theobromae plays a significant role as one of the most virulent grapevine pathogens in the world. In this study, we evaluate the effects of temperature on phytotoxin‐induced virulence in Lasiodiplodia theobromae. The fungal strain is grown in Czapek‐Dox liquid medium at four different temperatures (20°C, 25°C, 30°C and 35°C) for 14 days under dark conditions. The culture filtrates were screened for three selected phytotoxins, indole‐3‐carboxylic acid, jasmonic acid, and lasiodiplodin using UPLC MS/MS techniques. Phytotoxicity of the crude culture filtrates was tested on detached grapevine leaves. With UPLC MS/MS analysis, we identified that indole‐3‐carboxylic acid and jasmonic acid production varied with the increasing temperature, and did not detect any lasiodiplodin. At high temperatures, we observed a significant increase in the phytotoxicity of crude culture filtrates on the detached grapevine leaves. The most severe necrotic leaf lesions were observed when inoculated with crude culture filtrates extracted at 35°C.

Dual RNA-Seq Reveals Temperature-Mediated Gene Reprogramming and Molecular Crosstalk between Grapevine and Lasiodiplodia theobromae

“…However, the molecular mechanism of how grapevine responds to L. theobromae remains to be elucidated. In this study, we first tested and verified that high temperature is conducive to the disease development caused by L. theobromae, which is in accord with the results of previous reports [28,43].…”

An NmrA-Like Protein, Lws1, Is Important for Pathogenesis in the Woody Plant Pathogen Lasiodiplodia theobromae

“…Importantly, several virulence factors, such as LtLysM1 [42], LtEpg1 [43], and LtCFEMs [35], have been cloned and characterized in detail. Moreover, Aluthmuhandiram et al [44] isolated two phytotoxins, namely, indole-3-carboxylic acid and jasmonic acid, and tested their virulence against grapevine. In the future, more attention should be paid to investigating the colonization process and pathogenicity mechanism if we aspire to defeat this fungus.…”