Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species

Funnekotter, Bryn; Kaczmarczyk, Anja; Turner, Shane R.; Bunn, Eric; Zhou, Wenxu; Smith, Steven M.; Flematti, Gavin R.; Mancera, Ricardo L.

doi:10.1007/s11240-013-0308-0

Cited by 24 publications

(24 citation statements)

References 64 publications

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“…However, in the case of L. sonderi a 464 large 50% decrease in DBI was observed after preconditioning at 20/-1 °C (9.93 ± 0.81) compared to 465 preconditioning at 23 °C (20.32 ± 1.23). This trend was similarly observed by Funnekotter et al (2013) …”

Section: Control (-Ln) Shoot Tip Survival 443supporting

confidence: 80%

“…by Funnekotter et al (2013) in G. scapigera and L. cinerea, which showed increases in total sugar content 500 after preconditioning at 20/10 °C. These observations relate to previous findings, which showed an 501 increase in sugar content as a survival response to abiotic stresses (Sasaki et al 1996, Zhu et al 2006.…”

Section: Control (-Ln) Shoot Tip Survival 443mentioning

confidence: 96%

“…Sterols and phospholipids were analysed as described by Funnekotter et al (2013). L. sonderi 233 shoot tips from the two temperature preconditioning regimes were isolated (9-11 mg, ~12 shoot tips).…”

Section: Extraction Of Sterols and Phospholipids 232mentioning

confidence: 99%

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Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Menon

Funnekotter

Kaczmarczyk

et al. 2014

Plant Cell Tiss Organ Cult

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Cold-induced changes affect survival after exposure to vitrification solution during Abstract 35There is limited knowledge of the effects of exposure to low temperatures in the unique Mediterranean 36 climate plant species of Western Australia. We have thus investigated the effect of low temperature on 37 cryogenic tolerance in Lomandra sonderi, an endemic perennial species of southwest Western Australia.

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Section: Control (-Ln) Shoot Tip Survival 443supporting

confidence: 80%

Section: Control (-Ln) Shoot Tip Survival 443mentioning

confidence: 96%

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Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Menon

Funnekotter

Kaczmarczyk

et al. 2014

Plant Cell Tiss Organ Cult

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“…The preparation of artificial membrane model is relatively simple, even for more lipid complex compositions, and the only requirement is to know the composition of the biological target membrane (e.g., the plant PM). Because the lipid composition of a membrane is plant-, tissue-, or even organelle-specific, this information may be scarce but a few examples exist in the literature (see, for instance, References [43][44][45] for A. thaliana). Of course, the closer to the real membrane the lipid composition is, the more it will be biologically relevant, with the important caveat that increased complexity of the model will result in a more complex interpretation of the biophysical data (e.g., Reference [38] for NMR).…”

Section: Biomimetic Membrane Modelsmentioning

confidence: 99%

Contributions and Limitations of Biophysical Approaches to Study of the Interactions between Amphiphilic Molecules and the Plant Plasma Membrane

Furlan

Laurin

Botcazon

et al. 2020

Plants

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Some amphiphilic molecules are able to interact with the lipid matrix of plant plasma membranes and trigger the immune response in plants. This original mode of perception is not yet fully understood and biophysical approaches could help to obtain molecular insights. In this review, we focus on such membrane-interacting molecules, and present biophysically grounded methods that are used and are particularly interesting in the investigation of this mode of perception. Rather than going into overly technical details, the aim of this review was to provide to readers with a plant biochemistry background a good overview of how biophysics can help to study molecular interactions between bioactive amphiphilic molecules and plant lipid membranes. In particular, we present the biomimetic membrane models typically used, solid-state nuclear magnetic resonance, molecular modeling, and fluorescence approaches, because they are especially suitable for this field of research. For each technique, we provide a brief description, a few case studies, and the inherent limitations, so non-specialists can gain a good grasp on how they could extend their toolbox and/or could apply new techniques to study amphiphilic bioactive compound and lipid interactions.

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“…Temperature preconditioning is commonly used as a method to increase cell membrane stability at low temperatures through increased levels of unsaturated acyl chains (Menon et al 2014). Studies on two Australian species (Grevillea scapigera and Loxocarya cinerea) exposed to 20/10 C alternating temperature preconditioning resulted in a decrease in common acyl chains (16 : 0, 18 : 0, 18 : 1 and 18 : 2) with an increase in a-linoleyl tails (18 : 3) (Funnekotter et al 2013). Larger concentrations of the more unsaturated a-linoleyl tails induce a greater level of membrane molecular disorder, leading to increased membrane fluidity, stabilisation of the lamellar phase and maintenance of diffusion across membranes when subjected to reduced temperatures and desiccation.…”

Section: The Role Of Cell Membranes In Cryopreservationmentioning

confidence: 99%

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

et al. 2019

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An alarming proportion of Australia’s unique plant biodiversity is under siege from a variety of environmental threats. Options for in situ conservation are becoming increasingly compromised as encroaching land use, climate change and introduced diseases are highly likely to erode sanctuaries regardless of best intentions. Ex situ conservation is currently limited to botanic garden living collections and seed banking, with in vitro and cryopreservation technologies still being developed to address ex situ conservation of species not amenable to conventional storage. Cryopreservation (storage in liquid nitrogen) has been used successfully for long-term biosecure storage of shoot tips of several species of threatened Australian plants. We present a case for building on this research and fostering further development and utilisation of cryopreservation as the best means of capturing critical germplasm collections of Australian species with special storage requirements (e.g. recalcitrant-seeded taxa and species with short-lived seeds) that currently cannot be preserved effectively by other means. This review highlights the major issues in cryopreservation that can limit survival including ice crystal damage and desiccation, toxicity of cryoprotective agents, membrane damage, oxidative stress and mitochondrial function. Progress in understanding and mitigating these stresses is vital for advancing cryopreservation for conservation purposes.

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Acclimation-induced changes in cell membrane composition and influence on cryotolerance of in vitro shoots of native plant species

Cited by 24 publications

References 64 publications

Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Cold-induced changes affect survival after exposure to vitrification solution during cryopreservation in the south-west Australian Mediterranean climate species Lomandra sonderi (Asparagaceae)

Contributions and Limitations of Biophysical Approaches to Study of the Interactions between Amphiphilic Molecules and the Plant Plasma Membrane

Current issues in plant cryopreservation and importance for ex situ conservation of threatened Australian native species

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