Accession-specific modifiers act with ZWILLE/ARGONAUTE10 to maintain shoot meristem stem cells during embryogenesis in Arabidopsis

Tucker, Matthew R.; Roodbarkelari, Farshad; Truernit, Elisabeth; Adamski, Nikolai M; Hinze, Annika; Lohmüller, Barbara; Würschum, Tobias; Laux, Thomas

doi:10.1186/1471-2164-14-809

Cited by 14 publications

(10 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Very recently, a RNA-based sequence analysis revealed an upregulation (over eightfolds) of germin-like protein genes (ZmGLP2-1) during maize embryogenic tissue culture initiation (Salvo et al 2014). In Arabidopsis, the germin-like protein 6 (AT5G39100) and a putative GLP (AT5G38960) were differentially expressed in torpedo stage embryos (Tucker et al 2013). Arabidopsis calmodulin N-methyltransferase (CaM KMT), involved in transfer of methyl groups to calmodulin (CaM), was highly expressed in the apical meristemic region of plants during active growth stages (Banerjee et al 2013).…”

Section: Glp Expression At Early Developmental Stagesmentioning

confidence: 99%

Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

View full text Add to dashboard Cite

Germin-like proteins (GLPs) are evolutionary conserved ubiquitous plant glycoproteins belonging to the cupin superfamily. A large number of GLP family members have been identified from different higher and lower plant species, and those have been classified into different subfamilies. Although three histidine residues (H) and one glutamate residue (E) in germin box B and C were conserved among all the GLP subfamily members, how the sequences of one subfamily member differ from the other is unclear. Progress in the field of genomics, transcriptomics, and proteomics has made it possible to understand the variation at gene level among different GLP members from diverse genera and also their biological significances. GLPs from different plant species were found to have various enzymatic properties including oxalate oxidase (OxO), superoxide dismutase (SOD), ADP glucose pyrophosphatase/phosphodiesterase (AGPPase), and polyphenol oxidase (PPO) activities. 'Omics' study demonstrated the expression as well as involvement of GLP family members in almost every part of higher plants as well as in lower plants. Additionally, GLPs from different species were reported to be involved in biotic as well as abiotic stresses and also in the growth and development. This review describes the present research status of GLPs from different plant species, their expressions, and functional significances. Sequence variation was detected among GLP subfamily members at the amino acid level, and based on the sequence variation and phylogenetic analyses, two new GLP subfamilies have been proposed in this review.

show abstract

Section: Glp Expression At Early Developmental Stagesmentioning

confidence: 99%

Versatility of germin-like proteins in their sequences, expressions, and functions

Barman

Banerjee

2015

Funct Integr Genomics

View full text Add to dashboard Cite

show abstract

“…In particular, the mutation in ZWILLE / PINHEAD / AGO10 ( ZLL ) shows defects in maintenance of embryonic shoot apical meristem. However, these defects were apparent in Ler, but not in Col-0, because the defects were masked by the modifiers in Col-0 ( Tucker et al, 2013 ). One of the modifiers in the ZLL function appeared to be the Arabidopsis Cyclophilin-40 orthologue SQUINT (SQN, AT2G15790) ( Tucker et al, 2013 ).…”

Section: Discussionmentioning

confidence: 98%

“…However, these defects were apparent in Ler, but not in Col-0, because the defects were masked by the modifiers in Col-0 ( Tucker et al, 2013 ). One of the modifiers in the ZLL function appeared to be the Arabidopsis Cyclophilin-40 orthologue SQUINT (SQN, AT2G15790) ( Tucker et al, 2013 ). In addition, the altered meristem program ( amp1 ) mutation differently affected the flowering time in Col-0 and Ler ( Lee, 2009 ), presumably because of the different genetic modifiers in these accessions.…”

Section: Discussionmentioning

confidence: 98%

Accession-Dependent CBF Gene Deletion by CRISPR/Cas System in Arabidopsis

Cho

Park

et al. 2017

Front. Plant Sci.

View full text Add to dashboard Cite

The CRISPR/Cas system became a powerful genome editing tool for basic plant research and crop improvement. Thus far, CRISPR/Cas has been applied to many plants, including Arabidopsis, rice and other crop plants. It has been reported that CRISPR/Cas efficiency is generally high in many plants. In this study, we compared the genome editing efficiency of CRISPR/Cas in three different Arabidopsis accessions [Col-0, Ler, and C24RDLUC (C24 accession harboring the stress-responsive RD29A promoter-driven luciferase reporter)]. For the comparison, we chose to target the cold-responsive C-repeat/DRE-Binding Factor (CBF) genes. CBF1, CBF2, and CBF3 genes are tandemly located on Arabidopsis chromosome 4 with redundant functions as the key transcription factors functioning in cold stress signaling and tolerance. Due to the close proximity of these CBFs on the chromosome, it is impossible to generate cbf1, cbf2, cbf3 triple mutants (cbf123) by traditional genetic crosses. Therefore, using the CRISPR/Cas tool, we aimed to generate cbf123 mutants and compared the genome editing efficiency in different Arabidopsis accessions. Among the accessions, Ler was the most resilient to the CRISPR/Cas deletion with the lowest gene deletion ratio in both T1 and T2 generations. Interestingly, while C24RDLUC showed a high CBF123 deletion frequency in T2 only when the gene deletion was observed in T1 generation, Col-0 displayed high ratios of the CBF123 deletions in T2 regardless of the presence or absence of the CBF123 deletion in T1. Isolated cbf123 mutants in C24RDLUC background showed no expression of CBF1, CBF2, and CBF3 genes and proteins with reduction in the CBF target gene expression under cold stress.

show abstract

“…In the L er accession that has higher SQN expression levels and thus a higher potential for AGO1-mediated slicing of HD-ZIP III mRNAs, the decoy function of ZLL/AGO10 is crucial for shoot meristem stem cell maintenance during embryogenesis. By contrast, in the Columbia accession that has lower SQN expression, ZLL/AGO10 is dispensable for this process ( Tucker et al., 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Dose-Dependent AGO1-Mediated Inhibition of the miRNA165/166 Pathway Modulates Stem Cell Maintenance in Arabidopsis Shoot Apical Meristem

Gong

Boscá

et al. 2020

Plant Communications

Self Cite

View full text Add to dashboard Cite

Pluripotent stem cells localized in proliferating growth centers, the meristems, are the origin of life-long organ formation and growth in higher plants. In the shoot apical meristem of Arabidopsis thaliana , the closely related ARGONAUTE proteins AGO1 and ZLL/AGO10 bind miR165/166 species to regulate mRNAs of HD-ZIP III transcription factors that are essential to maintaining stem cells. Several genetic studies showed that AGO1 and ZLL/AGO10 act redundantly to maintain stem cells. By contrast, the reported biochemical data suggested antagonistic functions: AGO1 utilizes miR165/166 to slice HD-ZIP III mRNAs, whereas ZLL/AGO10 promotes degradation of miR165/166 and thus stabilizes HD-ZIP III mRNAs. How these different functions are balanced in stem cell regulation has remained enigmatic. Here, we show that autorepression of AGO1 through miR168-mediated slicing of its own RNA is required to maintain the ability of AGO1 to suppress HD-ZIP III mRNAs. Increased AGO1 expression, either in the miR168a-2 mutant or by transgenic expression, inhibits this ability despite the presence of high levels of miR165/166, effectively uncoupling HD-ZIP III and miR165/166 expression. AGO1 activity can be restored, however, by increasing the levels of chaperones SQN and HSP90, which promote assembly of RNA-induced silencing complex (RISC). This suggests that cellular abundance of SQN and HSP chaperones limits AGO1-mediated RNA interference in shoot meristem stem cell regulation. Localized misexpression of AGO1 indicates that the cells surrounding the shoot meristem primordium play a crucial role in stem cell development. Taken together, our study provides a framework that reconciles biochemical and genetic data, showing that restriction of AGO1 levels by miR168-mediated autorepression is key to RISC homeostasis and the function of AGO1 in stem cell regulation.

show abstract

Accession-specific modifiers act with ZWILLE/ARGONAUTE10 to maintain shoot meristem stem cells during embryogenesis in Arabidopsis

Cited by 14 publications

References 49 publications

Versatility of germin-like proteins in their sequences, expressions, and functions

Versatility of germin-like proteins in their sequences, expressions, and functions

Accession-Dependent CBF Gene Deletion by CRISPR/Cas System in Arabidopsis

Dose-Dependent AGO1-Mediated Inhibition of the miRNA165/166 Pathway Modulates Stem Cell Maintenance in Arabidopsis Shoot Apical Meristem

Contact Info

Product

Resources

About