Accessing microbial diversity for bioremediation and environmental restoration

Paul, Debarati; Pandey, Gyanendra; Pandey, Janmejay; Jain, Rakesh

doi:10.1016/j.tibtech.2005.01.001

Cited by 236 publications

(89 citation statements)

References 70 publications

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“…Adanya identifikasi jenis bakteri pada lahan bekas tambang sangat berguna untuk menentukan pemilihan bakteri isolat lokal yang akan digunakan dalam proses bioremediasi. Keragaman jenis bakteri yang diisolati dari lokasi pasca tambang diduga mampu memperbesar kemungkinan ditemukannya bakteri potensial sebagai agen bioremediasi logam berat (Paul et al, 2005). Keberadaan bakteri isolat lokal diduga memiliki kemampuan yang baik dan lebih efektif untuk mereduksi cemaran karena telah mengalami adaptasi dengan lingkungan dan cekaman logam pada air di lahan pasca tambang.…”

Section: Pendahuluanunclassified

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

Christita¹,

Iwanuddin

Kafiar

et al. 2018

JWAS

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ABSTRAKKegiatan pertambangan nikel menyisakan dampak pencemaran logam berat baik pada tanah maupun badan air. Salah satu metode yang banyak diaplikasikan untuk mengurangi cemaran logam berat adalah bioremediasi. Pemilihan bakteri yang berpotensi mengurangi kontaminasi logam berat di tanah sangat penting dalam proses bioremediasi. Langkah awal untuk memilih bakteri potensial adalah dengan melakukan identifikasi bakteri. Penelitian ini bertujuan untuk mengidentifikasi bakteri yang ada pada air kolam bekas galian di lahan bekas tambang nikel di Halmahera Timur. Metode yang digunakan dalam penelitian ini adalah isolasi dan identifikasi bakteri dengan metode konvensional meliputi uji morfologi, fisiologis, dan biokimia. Isolat bakteri diidentifikasi menggunakan Bergey's Manual Determinative. Hasil penelitian menunjukkan terdapat 6 genus bakteri yaitu Bacillus sp., Esherichia, Enterococcus, Pseudomona,s Staphylococcus, dan Klebsiella dengan 18 spesies bakteri pada air di lahan pasca tambang nikel. Secara morfologis didominasi oleh genus bakteri Bacillus sebesar 50 %. Hasil identifikasi bakteri ini membuktikan adanya bakteri indegenus yang resisten terhadap cekaman logam berat. Kata kunci: bakteri, tanah, air, kontaminasi, pasca tambang, Halmahera Timur ABSTRACT Nickel mining impact in heavy metal pollution on both soil and water. A method that is widely applied to reduce heavy metal contamination is bioremediation. Selection of bacteria that have potential reduction of heavy metal contamination in soil is very important in bioremediation process. The first step to select the potential bacteria is identification of samples. The purpose of this study is to identify existings bacteria in pond after

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Section: Pendahuluanunclassified

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

Christita¹,

Iwanuddin

Kafiar

et al. 2018

JWAS

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“…The microbial transformation may be driven by energy needs, or a need to detoxify the pollutants, or may be fortuitous in nature (cometabolism). Because of the ubiquitous nature of microorganisms, their numbers and large biomass relative to other living organisms in the earth, wider diversity and capabilities in their catalytic mechanisms (Paul et al, 2005), and their ability to function even in the absence of oxygen and other extreme conditions the search for pollutant-degrading microorganisms, understanding their genetics and biochemistry, and developing methods for their application in the field have become an important human endeavour (Megharaj et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Pesticide using Fungi Isolated from Paddy Fields of Thanjavur District, India

Sangeetha¹,

Kanimozhi²,

Panneerselvam³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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“…Although numerous studies focused on biofilm reactor in the field of bioremediation [3], pollution by oil and other hydrocarbons solely during the oil spillage needs further attention in the context of bioavailability of microorganisms. It has been investigated earlier that this major limitation can be improved by exploiting chemotactic bacteria [4][5][6]. Microbial chemotaxis plays important role in surface colonization and biofilm formation [7].…”

Section: Introductionmentioning

confidence: 99%

Chemotaxis of Biofilm Producing <i>Pseudomonas</i> spp. towards Refined Petroleum Oil

Dutta

Singh

2016

J. Sci. Res.

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The bioavailability of organic contaminants to the degrading bacteria is a major limitation to efficient bioremediation of sites contaminated with hydrophobic pollutants. Studies were conducted to analyze the presence of biodegrading bacteria on the sites of refinery and garage oil spills with abundance of hydrocarbons. Contaminated soils were collected from these rich hydrocarbon sites and bacteria were isolated from samples using regular bacterial media enriched with petroleum oil. These isolates were characterized for their efficiency to utilize refining petroleum oil as their energy source and then the most common isolate was characterized. Further investigations examine the formation of biofilm by naturally existing oil-degrading bacteria on refine petroleum oil degradation. Here also studied the abilities of these Pseudomonas strains to respond chemo tactically to refine petroleum oil. Microbial chemotaxis plays important role for formation of biofilm. 16S rDNA analysis of the best degrader was found to belong to the Pseudomonas species. Interestingly, one of the best isolates was found to be close to Pseudomonas aeruginosa family.

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Accessing microbial diversity for bioremediation and environmental restoration

Cited by 236 publications

References 70 publications

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

Identification of Water Bacteria from Nickel Post Mining in East Halmahera

Biodegradation of Pesticide using Fungi Isolated from Paddy Fields of Thanjavur District, India

Chemotaxis of Biofilm Producing <i>Pseudomonas</i> spp. towards Refined Petroleum Oil

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