Accelerator Analysis of Tributyltin Adsorbed onto the Surface of a Tributyltin Resistant Marine Pseudoalteromonas sp. Cell

Abstract

Tributyltin (TBT) released into seawater from ship hulls is a stable marine pollutant and obviously remains in marine environments. We isolated a TBT resistant marine Pseudoalteromonas sp. TBT1 from sediment of a ship's ballast water. The isolate (10 9.3 ± 0.2 colony-forming units mL -1 ) adsorbed TBT in proportion to the concentrations of TBTCl externally added up to 3 mM, where the number of TBT adsorbed by a single cell was estimated to be 10 8.2 . The value was reduced to about one-fifth when the lysozyme-treated cells were used. The surface of ethanol treated cells became rough, but the capacity of TBT adsorption was the same as that for native cells. These results indicate that the function of the cell surface, rather than that structure, plays an important role to the adsorption of TBT. The adsorption state of TBT seems to be multi-layer when the number of more than 10 6.8 TBT molecules is adsorbed by a single cell.

Biotransformation of tributyltin chloride to less toxic dibutyltin dichloride and monobutyltin trichloride by Klebsiella pneumoniae strain SD9

“…Such drastic alterations in cell size are a protective mechanism in several bacteria subjected to heavy-metal and organotin stress (Mimura et al, 2008;Naik and Dubey, 2011;Shamim et al, 2013). A similar response was observed in P. aeruginosa strain 4EA under the stress of lead (Naik and Dubey, 2011).…”

“…The role of pyochelin in conferring resistance to TPT has also been demonstrated in Pseudomonas aeruginosa (Sun et al, 2006). Both pyochelin and pyoverdine siderophores have also been implicated in lead resistance in P. aeruginosa strain 4EA (Naik and Dubey, 2011). Similarly, TBTCl-degrading marine Pseudomonas spp.…”

“…Therefore there is pressing need to clean up TBTClcontaminated estuarine environments using a biotechnological approach without affecting environmental sustainability. Despite TBTCl toxicity to several organisms, TBTCl resistance has been reported in some bacterial isolates (Wuertz et al, 1991;Fukagawa et al, 1994;Dubey and Roy, 2003;Mimura et al, 2008;Ramachandran and Dubey, 2009;Fukushima et al, 2012;Shamim et al, 2013). Interestingly, there are some reports on marine and estuarine bacterial strains resisting TBTCl and there is evidence suggesting that biodegradation is the major breakdown pathway in estuarine and marine sedimentary environment (Wuertz et al, 1991;Fukagawa et al, 1992;Dubey and Roy, 2003;Mendo et al, 2003;Cruz et al, 2007Cruz et al, , 2010a2014;Lee et al, 2012;Sampath et al, 2012).…”

Microconstituents: What to Expect in Your Permit

“…Concentrations as high as 500 ng/L have been detected in coastal waters (Alzieu et al, 1989). Tributyltin is considered an endocrine disruptor (Mimura et al, 2008) to aquatic organisms due to its ability to produce male sexual characteristics in female abalones (a type of sea snail) at 100 ng/L (Horiguchi et al, 2002) and gastropods (also a type of snail) (Horiguchi, 2006). Numerical standards for tributyltin exist in states such as Colorado, which were established from EPA's National Recommended Water Quality Criteria.…”

Sorption of Triphenyltin Chloride to nFe3O4, Fly Ash, and nFe3O4/Fly Ash Composite Material in Seawater

“…TPT therefore contaminates the marine environment during painting and hosing activities in the form of paint particles at the harbor/dockyard. Although banned in some countries on vessels <25 m in length , studies have found high concentration of organotins (TPT and TBT (tributyltin)) in tissues of marine mammals, their presence has been linked to imposex formation in mollusks and mass mortalities of marine mammals , and in human, consumption of seafood is thought to be the possible source of TPT , hence, a need to treat shipyard process wastewater free from TPT before discharge into the marine environment.…”