Accelerating skin barrier repair using novel bioactive magnesium-doped nanofibers of non-mulberry silk fibroin during wound healing

Abstract

Novel magnesium doped non-mulberry silk fibroin nanofibers with ability to enhance skin barrier function were successfully fabricated using electrospinning technique for wound healing applications. Magnesium nanoparticles incorporated in the electrospun nanofibers releases Mg2+ ions at the site of implementation. The effect of Mg2+ is of considerable concern in wound healing due to its skin barrier repair ability and its role in blood coagulation. The physicochemical characterization of the scaffold was investigated by determining the morphology and secondary structure confirmation. The effects of Mg2+ ions in silk fibroin microenvironment have been evaluated using SEM, XRD, and FTIR to confirm the incorporation of magnesium in the film. The aim of this study is to see the effect of doped Mg on the structural, physical, and biological properties of non-mulberry silk fibroin (NSF) film. The magnesium doped nanofibrous film exhibited enhanced mechanical property, satisfactory blood clotting ability, and good in vitro degradability. This silk fibroin-based film mimicking extracellular matrix for skin regeneration were constructed using electrospinning technique. The wound healing efficiency of prepared nanofibers were evaluated in full-thickness wound models of rat. The Mg doped silk fibroin film exhibited faster wound healing activity (14 days) among all experimental group. The study indicates the potential of magnesium-doped silk /PVA film as skin substitute film.

Fibroblast Derived Skin Wound Healing Modeling on Chip under the Influence of Micro-Capillary Shear Stress

“…Characterization of prepared silk film: FTIR spectra of transparent silk fibroin film in the range of 800 to 2000 cm −1 were obtained using Attenuated Total Reflectance (ATR) mode. The FTIR curves were characterized by absorption peaks at 1651 cm −1 (amide I), 1542 cm −1 (amide II), and 1235 cm −1 (amide III), confirming the presence of the various secondary structure of silk fibroin [20] (as shown in Figure 9). X-ray diffraction (XRD) of the sample was carried out at range 2θ = 10 to 60°.…”

“…X-ray diffraction (XRD) of the sample was carried out at range 2θ = 10 to 60°. The XRD curve shows a peak at angle 19.9 confirming the presence of silk fibroin in the film, as shown in Figure 10 below [20]. When media was injected through two-side channels, an artificial wound was created in the middle layer.…”

“…Wounds of limited (confined within the width of the microchannel) cross-sectional area were prepared due to dependency on media flow pattern for wound X-ray diffraction (XRD) of the sample was carried out at range 2θ = 10 to 60 • . The XRD curve shows a peak at angle 19.9 confirming the presence of silk fibroin in the film, as shown in Figure 10 below [20]. When media was injected through two-side channels, an artificial wound was created in the middle layer.…”

“…The obtained film prepared using the solvent evaporation method was treated with 70% ethanol to induce crystallinity. This allows the formation of a water-insoluble silk film due to the induction of β-sheet protein secondary structure [20]. Films were then washed with sterile phosphate buffer saline (PBS) before performing the in vitro study.…”

Antheraea pernyi silk fibroin-coated adenovirus as a VEGF165-Ang-1 dual gene delivery vector

“…Silk fibroin extracted from silk has no obvious immunogenicity, has good biocompatibility and can be completely biodegraded, and has been widely used in tissue engineering scaffolds and drug delivery carriers. [26][27][28] Antheraea pernyi silk is the only race of wild silkworm silk used in large-scale industrialization. Compared with domestic silk fibroin, Antheraea pernyi silk fibroin (ASF) contains more residues of acidic amino acids, basic amino acids, and polar amino acids.…”