Accelerating column chromatography: the bundled approach

Abstract

The use and construction of a novel apparatus for a variant of conventional column chromatography is described. Relative to a single large conventional column, a bundled apparatus facilitates scale-up by accelerating fraction collection. The bundled approach permits prior knowledge of total running time, total solvent volume, and product location.

α-Benzoyloxylation of dimethyl trisulfide and a novel reaction of the resultant trisulfide benzoate

“…Conventional column chromatography on 1 led to some material loss (apparent decomposition) and, subsequently, the development of an apparatus for bundled chromatography (3). Preparation of 1 followed up with bundled column chromatography and reduced pressure distillation (4) provided material of satisfactory purity, albeit in somewhat reduced yield (19%).…”

Structure‐function studies for in vitro chemical inhibition of Fcγ receptor–mediated phagocytosis

“…Fractions 36 to 57 were combined and concentrated, affording impure p ‐nitrobenzyl methyl sulfide (1.45 g). Impure chromatographed product was rechromatographed on a four‐bundle system 6 employing petroleum ether. Fractions 155 to 209 were combined and concentrated, affording clean p ‐nitrobenzyl methyl sulfide (0.46 g, 2.5 mmol, 11%).…”