Accelerated wound healing process in rat by probiotic Lactobacillus reuteri derived ointment

Abstract

Background Antioxidant agents have positive effects on wound healing process and on the other hand probiotics also have great antioxidation or oxidation-resistance properties. Methods In this study, we investigated the effects of Lactobacillus reuteri derived ointment as a probiotic bacterium on the wound healing process using a full-thickness rat skin model. The wound diameter and contraction percent measurement, histopathological examination, and myeloperoxidase (MPO) enzyme activity evaluation were carried out during the period of study. Results Amount of deposit collagen was significantly increased; epithelization speed and epidermis production were also observed in the ointment-treated group, while the inflammation rate was also significantly lower than the control group. In addition, a significant reduction in the activity of enzyme MPO was also detected in the ointment-treated group. Conclusions According to the results, probiotic ointment effectively accelerates the wound healing process and it can be used as a promising drug for wound dressing application.

Development and In Vivo Characterization of Probiotic Lysate-Treated Chitosan Nanogel as a Novel Biocompatible Formulation for Wound Healing

“…Even after 14 days (the end of the experiment), the healing process in the control group was not completed (Table 3). Khodaii et al reported that administration of L. reuteri extract significantly promoted the wound healing processes by day 15, which is comparable with our results (the healing process was completed before ten days) [15]. In this regard, Han et al claimed that L. reuteri enhanced wound healing via the PI3K/AKT/β-catenin/TGFβ1 pathway [16].…”

Improvement of Cutaneous Wound Healing via Topical Application of Heat-Killed Lactococcus chungangensis CAU 1447 on Diabetic Mice

“…rhamnosus R0011, and Lb. reuteri ) decreased the MPO level [ 33 , 34 , 35 ]. Therefore, a topical postbiotic application may accelerate wound healing by decreasing MPO activity from day 5 onward and rapidly proceed to the next stage, namely, the proliferation phase, and beyond the inflammatory phase.…”

Effect of White Tea (Camellia sinensis) Extract on Skin Wound Healing Process in Rats

“…Skin protects our bodies against dehydration and foreign pathogens influx and maintains body homeostasis and temperature 1 , 2 . Therefore, wounds by causing a disturbance in skin anatomy and function may lead to complications such as infection and even shock 3 , 4 .…”

“…Therefore, wounds by causing a disturbance in skin anatomy and function may lead to complications such as infection and even shock 3 , 4 . Because of the importance of healthy skin, wounds prevalence and the costs of skin wound treatment, it is essential to find a low-cost and effective treatment for skin wounds 2 , 5 , 6 .…”

“…Considering similarities between green tea and white tea, and benefits of green tea ethanolic extract on the surgical and burn wounds 1 , 21 , more anti-oxidants in white tea in comparison with green tea and the positive effects of antioxidants and antimicrobial agents on the wound healing process and all the properties of white tea, we aimed to evaluate the effect of white tea extract on the healing process of excisional skin wounds in rats 2 , 14 , 15 , 18 .…”