Accelerated low-intensity rTMS does not rescue anxiety behaviour or abnormal connectivity in young adult rats following chronic restraint stress

Hennessy, Lauren A.; Seewoo, Bhedita J.; Jaeschke, Liz A.; Mackie, Leah A.; Figliomeni, Abbey; Arena-Foster, Yasmin; Etherington, Sarah J.; Dunlop, Sarah; Croarkin, Paul E.; Rodger, Jennifer

doi:10.1016/j.ynirp.2022.100104

Cited by 2 publications

(4 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Three studies (Artifon et al, 2020;Lubomski et al, 2022;Haney et al, 2018) did not report whether the neuromodulation intervention had an effect on disease outcome, although Haney et al (2018) do mention that the VNS stimulation parameters were also used previously in their experiments to promote swallowing and upper airway function in SOD1-G93A mice (Lever et al, 2010) (see Table 3). Disease outcome of the rats in the study by Seewoo et al (2022) was later reported in Hennessy et al (2022) where it was found that rTMS did not rescue depressive-like behaviors induced with chronic restraint stress, as measured on the forced swim test. The other three studies found that the neuromodulation interventions significantly reduced severity of disease-related symptoms (Kanayama et al, 2019;Ferrulli et al, 2021;Phillips Campbell et al, 2016).…”

Section: Disease Outcome After Neuromodulationmentioning

confidence: 90%

Are neuromodulation interventions associated with changes in the gut microbiota? A systematic review

et al. 2023

View full text Add to dashboard Cite

Section: Disease Outcome After Neuromodulationmentioning

confidence: 90%

Are neuromodulation interventions associated with changes in the gut microbiota? A systematic review

et al. 2023

View full text Add to dashboard Cite

“…First, only male late adolescent/young adult Sprague–Dawley rats were used in the analysis due to data availability. This means that the brains of these animals were still developing during the CRS intervention and may have confounded interpretation of the network changes ( Hennessy et al, 2022 ). However, this age range is relevant to studying mood disorders in young people.…”

Section: Discussionmentioning

confidence: 99%

“…The MRI data analysed in the present study was a combined dataset of rodent cohorts from previous experiments conducted by our lab in 2019 and 2020. Briefly, 109 male Sprague—Dawley rats (aged 6–7 weeks and weighing 150–250 g on arrival; N = 12 from Seewoo et al (2020) , N = 56 from Hennessy et al (2022) and N = 41 unpublished) were sourced from the Animal Resources Centre (Canning Vale, WA). All rats were housed in pairs under a standard 12-h light–dark cycle with ad libitum food and water, in a temperature-controlled and spacious laboratory room located at UWA’s Animal Care Unit, M block building (Nedlands, WA).…”

Section: Methodsmentioning

confidence: 99%

“…To date, limited rs-fMRI studies in animal models of depression have investigated functional alterations within and between RSNs in response to interventions that induce stress and anxiety ( Henckens et al, 2015 ; Grandjean et al, 2016 ; Nephew et al, 2018 ; Seewoo et al, 2020 ; Hennessy et al, 2022 ). Majority of these rodent studies have applied chronic restraint stress (CRS), which is a popular, simple, and validated depression model.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identifying reproducible resting state networks and functional connectivity alterations following chronic restraint stress in anaesthetized rats

et al. 2023

Self Cite

View full text Add to dashboard Cite

BackgroundResting-state functional MRI (rs-fMRI) in rodent models have the potential to bridge invasive experiments and observational human studies, increasing our understanding of functional alterations in the brains of patients with depression. A major limitation in current rodent rs-fMRI studies is that there has been no consensus on healthy baseline resting-state networks (RSNs) that are reproducible in rodents. Therefore, the present study aimed to construct reproducible RSNs in a large dataset of healthy rats and then evaluate functional connectivity changes within and between these RSNs following a chronic restraint stress (CRS) model within the same animals.MethodsA combined MRI dataset of 109 Sprague Dawley rats at baseline and after two weeks of CRS, collected during four separate experiments conducted by our lab in 2019 and 2020, was re-analysed. The mICA and gRAICAR toolbox were first applied to detect optimal and reproducible ICA components and then a hierarchical clustering algorithm (FSLNets) was applied to construct reproducible RSNs. Ridge-regularized partial correlation (FSLNets) was used to evaluate the changes in the direct connection between and within identified networks in the same animals following CRS.ResultsFour large-scale networks in anesthetised rats were identified: the DMN-like, spatial attention-limbic, corpus striatum, and autonomic network, which are homologous across species. CRS decreased the anticorrelation between DMN-like and autonomic network. CRS decreased the correlation between amygdala and a functional complex (nucleus accumbens and ventral pallidum) in the right hemisphere within the corpus striatum network. However, a high individual variability in the functional connectivity before and after CRS within RSNs was observed.ConclusionThe functional connectivity changes detected in rodents following CRS differ from reported functional connectivity alterations in patients with depression. A simple interpretation of this difference is that the rodent response to CRS does not reflect the complexity of depression as it is experienced by humans. Nonetheless, the high inter-subject variability of functional connectivity within networks suggests that rats demonstrate different neural phenotypes, like humans. Therefore, future efforts in classifying neural phenotypes in rodents might improve the sensitivity and translational impact of models used to address aetiology and treatment of psychiatric conditions including depression.

show abstract

Accelerated low-intensity rTMS does not rescue anxiety behaviour or abnormal connectivity in young adult rats following chronic restraint stress

Cited by 2 publications

References 75 publications

Are neuromodulation interventions associated with changes in the gut microbiota? A systematic review

Are neuromodulation interventions associated with changes in the gut microbiota? A systematic review

Identifying reproducible resting state networks and functional connectivity alterations following chronic restraint stress in anaesthetized rats

Contact Info

Product

Resources

About