Accelerated Detection of DNA on Membranes by Automated Enzyme-Linked Immunofiltration Assay

Aubert, Dominique; Toubas, D.; Foudrinier, F.; Villena, Isabelle; Gomez, Jean-Pierre; Marx, C; Lepan, H.; Lemaire, Pascale; Jacquier, P; Bonhomme, A.; Pinon, J. M.

doi:10.1006/abio.1997.2030

Cited by 8 publications

(2 citation statements)

References 20 publications

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“…The data show that adsorption and hybridization occur over periods approaching 8 h in TB buffer, or longer with other buffer solutions. Hybridization periods on the order of several hours are often encountered with solid-phase hybridizations such as, for example, on nylon membranes (12 h) . More importantly, the kinetic parameters obtained for adsorption mirror the tendency for adsorption; that is, the QD conjugates and buffer conditions that yielded the lowest steady-state adsorption also yielded the slowest rates of adsorption.…”

Section: Resultsmentioning

confidence: 81%

Adsorption and Hybridization of Oligonucleotides on Mercaptoacetic Acid-Capped CdSe/ZnS Quantum Dots and Quantum Dot−Oligonucleotide Conjugates

2006

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Interest in the unique optical properties of quantum dots (QDs) has resulted in the development QD-bioconjugates for imaging and diagnostics. Although these applications are numerous, considerably less is known about the interactions between QDs and biomolecules. In this work, we describe hydrogen-bonding interactions between oligonucleotides and CdSe/ZnS quantum dots capped with mercaptoacetic acid ligands. The strength of the interactions can be modulated by changes in the pH and ionic strength, the addition of formamide, and differences between ssDNA and dsDNA. Fluorescence resonance energy transfer experiments have shown that conjugated oligonucleotides adopt a conformation that lies across the surface of the QD. The hydrogen-bonding interactions also affect the kinetics of hybridization with QD-DNA conjugates and the thermal stability of QD-conjugated dsDNA. The former is analogous to conventional solid-phase hybridization, where stronger oligonucleotide adsorption leads to faster kinetics. With respect to the latter, interactions with the QD surface can sharpen the melt transition and alter the melt temperature of dsDNA. These effects are largely absent when adsorptive interactions are minimized.

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Section: Resultsmentioning

confidence: 81%

Adsorption and Hybridization of Oligonucleotides on Mercaptoacetic Acid-Capped CdSe/ZnS Quantum Dots and Quantum Dot−Oligonucleotide Conjugates

2006

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“…Recently, they have also found application in immunoblotting (Westerns) (5,6). In general, most blotting procedures involve electrophoretic separation of proteins or nucleic acids and their transfer to a membrane by capillarity or capillary action, vacuum, or an electric field (7). Alternatively, samples may be bound to a membrane using a dot blot apparatus (1).…”

mentioning

confidence: 99%

Nylon Membrane Support for Immobilizing Antigens with Low Binding Affinity for Conventional ELISA Plates

Conlon

Berríos

1998

Analytical Biochemistry

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DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX method

Amraee

Oloomi

Yavari

et al. 2017

Analytical Biochemistry

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Accelerated Detection of DNA on Membranes by Automated Enzyme-Linked Immunofiltration Assay

Cited by 8 publications

References 20 publications

Adsorption and Hybridization of Oligonucleotides on Mercaptoacetic Acid-Capped CdSe/ZnS Quantum Dots and Quantum Dot−Oligonucleotide Conjugates

Adsorption and Hybridization of Oligonucleotides on Mercaptoacetic Acid-Capped CdSe/ZnS Quantum Dots and Quantum Dot−Oligonucleotide Conjugates

Nylon Membrane Support for Immobilizing Antigens with Low Binding Affinity for Conventional ELISA Plates

DNA aptamer identification and characterization for E. coli O157 detection using cell based SELEX method

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