Accelerated Burn Wound Closure in Mice with a New Formula Based on Traditional Medicine

Abstract

BackgroundA combination of the oils of sesame, hemp, wild pistachio, and walnut has been used for treatment of skin disorders, including wound burns, in some parts of Kerman, Iran. Evaluation of this remedy in the form of a pharmaceutical formulation in animal models can pave the way for its future application in wound burn healing in humans.ObjectivesThis experimental study investigated the healing potential of a new formula (NF) based on folk medicine from Iran for the treatment of third degree burns in mice. The formula was a combination of the oils of four plants: sesame (Sesamum indicum L.), wild pistachio (Pistacia atlantica Desf.), hemp (Cannabis sativa L.), and walnut (Juglans regia L.)MethodsTwenty-four mice were selected based on simple random sampling. Twenty-five percent of the total body surface area was burned by exposure to boiling water, according to the Walker-Mason method. NF and silver sulfadiazine (the positive control) were applied topically twice a day for 21 days. The burned area in the negative control group was left untreated. Epithelialization time and the percent of wound contraction were measured during the treatment period. The process of wound repairing was evaluated using histological (H and E and trichrome staining) and immunohistological (anti-pancytokeratin) methods.ResultsWhen compared to the controls, NF significantly improved wound contraction after day 10. Epithelialization time in the NF group was significantly faster than in the other groups (20 vs. 25.5 days) (P < 0.001). Histopathological and immunohistochemical findings confirmed the efficacy of the NF.ConclusionsA new therapeutic remedy was introduced for the treatment of burn wounds. Further clinical and molecular studies are suggested to determine the exact mechanism(s) involved in the burn wound healing effect of NF.

Effect of Pistacia Atlantica Resin Oil on Anti-Oxidant, Hydroxyprolin and VEGF Changes in Experimentally-Induced Skin Burn in Rat

“…This new formula increased wound contraction and accelerated epithelialization. 21 Hamidi et al . showed that P. atlantica oil as gels ameliorated epithelialization which is as a marker of wound contraction.…”

“…The wound contractions was reported as percent and were calculates by following formula: 21 %wound contraction=(wound area [day 1]-wound area [day 14]/wound area [day 1]×100…”

Histopathological Evaluation of Burdock (Arctium lappa) Root Hydroalcoholic Extract on Wound Healing

“…Wound healing has different stages including inflammation, proliferation, and reconstruction, each consisting of several other steps, some of which overlaps with each other and thus are not easily separable (24,25). Therefore, the qualitative and quantitative improvement of each of these stages can accelerate wound healing and reduce its complications.…”

Animal Models of Burn Wound Management

“…This model resembles the clinical situation and provides an opportunity to examine or develop new strategies such as new antibiotics and immune therapy for handling burn wound. Moreover, a 25% third-degree burn injury was demonstrated by exposure to boiling water for examining the efficacy of new formula-based traditional medicine [34]. Although burn mouse model has its specific advantages, evidently this model fails to completely mimic the wound healing process of humans.…”