Effect of Pistacia Atlantica Resin Oil on Anti-Oxidant, Hydroxyprolin and VEGF Changes in Experimentally-Induced Skin Burn in Rat

Abstract

BACKGROUNDSevere burn damage and its consequences are life threatening which can complicate patients’ health. Medicinal and traditional plants are considered as safe, natural and inexpensive source of treatment for wide variety of diseases. This study assessed beneficial effect of Pistacia atlantica oil on rats burn wound healing and its potential effects on malondialdehyde (MDA), vasculoendothelial growth factor (VEGF), hydroxyprolin and antioxidant status in wound area.METHODSThirty male rats weighing 200±10 g were randomly divided into three groups (n=10) as follows. Group 1 underwent just burn injury, Group 2 underwent burn injury and received 150 mg/kg/day P. atlantica oil topically, and Group 3 underwent burn injury and received 150 mg/kg/day sulfadiazine cream topically. At the end of the study (day 14), wounded areas were measured and then skin in the burn damage were dissected and anti-oxidative parameter, MDA, VEGF and hydroxyprolin were evaluated. RESULTS P. Atlantica oil significantly increased antioxidant defense, VEGF, hydroxyprolin and reduced MDA levels. It could remarkably reduce wound size compared to burn control group. P. Atlantica oil showed more beneficial effects than sulfadiazine.CONCLUSION P. atlantica resin oil could be considered as a new therapeutic agent for treatment of injuries.

The Effect of Mentha Pulegium on Healing of Burn Wound Injuries in Rat

“…Moist exposed burn ointment is an oil-based ointment composed of sesame oil and some other plant ingredients. 10,11,[14][15][16] However, in our study, the simple dressing which was a combination of a sterile gauze and normal saline did not show any significant improvement in burn wounds. Therefore, the moisture alone is not a factor for improvement of burn wounds.…”

“…Herbal products such as moist exposed burn ointment, Aloe vera, Aloe littoralis, Aloe saponaria haw and green tea have been effective in treatment of burn injuries in animal models. [10][11][12][13][14][15][16][17][18]28,29 On the other hand, using stem cells or skin grafts in burn injuries is costly and limited for developing countries, therefore, alternative therapies for second degree burn wounds has become a reasonable modality for cure. 30 Our results showed that M. pulegium extract was beneficial in treating second degree burn injuries, but the side effects of this product are not yet determined.…”

“…13 There are other studies supporting the effect of herbal remedies in treatment of burn wounds. [14][15][16][17][18][19][20] Traditionally, an extract from the leaves of a plant called M. pulegium has been used to alleviate first and second degree burn wounds in north rural areas of Iran. This study was conducted to evaluate the effect of M. pulegium extract on second degree burn injuries in rats.…”

Carnitine effects on serum and pancreas inflammatory response in diabetic rats

“…Pancreas samples of animals were sonicated and homogenized by Ultrasonic Processor (Hielscher, UP200H) in cold PBS with pH=7.4. The homogenized samples were centrifuged 15 min at 4 °C (15000 rpm) and then the supernatants were collected and IL-1β, IL-6 and TNF-α were quantified by ELISA method [24,25].…”

“…After serum or supernatant loading into the wells and incubation, the next step is washing which followed by second antibody addition to the reaction mixture which results in ELISA sandwich formation and then yellow color generated after the addition of stop solution. Finally, the reaction color development in wells is measured at 450 nm by ELISA plate reader [25,26].…”