Acanthamoeba keratitis. Review of literature. Case reports

Skryabina, Yelena V.; Владимировна, Скрябина Елена; Astakhov, Yuriy Sergeyevich; Сергеевич, Астахов Юрий; Konenkova, Yanina S.; Станиславовна, Коненкова Янина; Varganova, Tatiana S.; Сергеевна, Варганова Татьяна; Petukhov, V. P.; Павлович, Петухов Владимир; Nokhrina, Kseniya V.; Викторовна, Нохрина Ксения; Dnestryanskaya, Kaleriya O.; Олеговна, Днестрянская Калерия

doi:10.17816/ov2019159-71

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Because of these variations, we used SEM to report the impact of the extract on the morphology of A. castellanii trophozoites and cysts. Skryabina et al 42 reported that the insufficiency of medical therapy frequently occurs due to the scanty absorption of local anti-Acanthamoebic drugs through www.nature.com/scientificreports/ thickened walls. Our findings showed rupture in the operculum and definite pore formation in the cysts treated with the extract, which might facilitate the diffusion and absorption of the extract.…”

Section: Discussionmentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

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Natural products play a significant role in providing the current demand as antiparasitic agents, which offer an attractive approach for the discovery of novel drugs. The present study aimed to evaluate in vitro the potential impact of seaweed Padina pavonica (P. pavonica) extract in combating Acanthamoeba castellanii (A. castellanii). The phytochemical constituents of the extract were characterized by Gas chromatography–mass spectrometry. Six concentrations of the algal extract were used to evaluate its antiprotozoal activity at various incubation periods. Our results showed that the extract has significant inhibition against trophozoites and cysts viability, with complete inhibition at the high concentrations. The IC50 of P. pavonica extract was 4.56 and 4.89 µg/mL for trophozoites and cysts, respectively, at 24 h. Morphological alterations of A. castellanii trophozoites/cysts treated with the extract were assessed using inverted and scanning electron microscopes and showed severe damage features upon treatment with the extract at different concentrations. Molecular Docking of extracted compounds against Acanthamoeba cytochrome P450 monooxygenase (AcCYP51) was performed using Autodock vina1.5.6. A pharmacokinetic study using SwissADME was also conducted to investigate the potentiality of the identified bioactive compounds from Padina extract to be orally active drug candidates. In conclusion, this study highlights the in vitro amoebicidal activity of P. pavonica extract against A. castellanii adults and cysts and suggests potential AcCYP51 inhibition.

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Section: Discussionmentioning

confidence: 99%

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Abdel-Hakeem,

Hassan,

Hifney

et al. 2024

Sci Rep

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“…Increased drug dosage is needed to overcome this barrier and become more effective however; using high doses has toxic and side effects. Moreover, the failure rate of medical therapy frequently occurs due to insufficient absorption of topical anti-Acanthamoebic medications through thicken sclera (Skryabina et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Also, Ismail et al (2018) stated that different concentrations of both aqueous and alcoholic extracts of Nigella sativa possessed more potent cysticidal effect on Acanthamoeba cysts with growth inhibition up to 100% compared to chlorhexidine 0.02% which gave growth inhibitory effect ranging from 56 to 80% throughout the experiment. Although chlorhexidine is used as an initial medication for Acanthamoeba keratitis, continued treatment may lead to destruction of the plasma membranes of ocular cells, epithelial ulceration, keratocytes disappearance and impairment in endothelial function (Skryabina et al 2019). Therefore, the importance of these natural agents is related not only for their cysticidal effect but also they may be used as template molecules for the production of novel therapies less toxic and free from side effects.…”

Section: Discussionmentioning

confidence: 99%

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts

et al. 2020

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This study evaluated in vitro effect of different concentrations of Aloe vera (A. vera) ethanol extract and honey against Acanthamoeba spp. cysts in comparison with chlorhexidine (the drug of choice for treatment of Acanthamoeba infection) at different incubation periods. Four different concentrations of the tested agents were used, 100, 200, 400, and 600 lg/ml for A. vera ethanol extract and 25, 50, 100, and 200 lg/ml for honey. Isolated Acanthamoeba spp. cysts from keratitis patients were incubated with different concentrations of the tested agents as well as chlorhexidine 0.02% (drug control) for different incubation periods (24, 48, 72 h). After each incubation period, the effect of A. vera extract and honey against Acanthamoeba cysts was assessed by counting the number of viable cysts, determining the inhibitory percentage and detecting the morphological alternations of treated cysts compared to non-treated and drug controls. Both A. vera ethanol extract and honey showed a concentration and time-dependent effect on the viability of Acanthamoeba cysts. In comparison with chlorhexidine (the drug control), A. vera ethanol extract possessed a potent cysticidal activity at all tested concentrations throughout different incubation periods, except for concentration 100 lg/ml which recorded the lower inhibitory effect. With increasing the dose of A. vera ethanol extract to 200, 400, 600 lg/ml, the recorded inhibitory percentages of Acanthamoeba cysts viability were 82.3%, 92.9% and 97.9% respectively, after 72 h compared to 76.3% of chlorhexidine. Similarly, honey at concentrations of 50-100 lg/ml gave higher inhibitory effect of 59% and 76.7%, respectively compared to chlorhexidine which showed an inhibitory percentage of 55.7% after 24 h. Meanwhile, the lowest tested concentration of honey (25 lg/ml) gave an inhibitory effect by 47.7-67% which was less than that of chlorhexidine throughout different incubation periods. With increasing the dose of honey to 200 lg/ml, the inhibitory effect was 98.9% after 72 h higher than that of chlorhexidine (76.9%). Using a scanning electron microscope, Acanthamoeba cysts treated by A. vera ethanol extract showed alternations in their shapes with flattening, collapsing, and laceration of their walls. Also, treated cysts by honey were highly distorted and difficult to identify because most of them were shrinkage and collapsed to a tiny size. On the other hand, chlorhexidine showed less structural and morphological changes of Acanthamoeba cysts. A. vera ethanol extract and honey had considerable cysticidal effects on Acanthamoeba cysts. They may give promising results for treatment of Acanthamoeba keratitis.

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Monitoring the antimicrobial activity of antiseptic eye drops

Околов

Николаевич²

2019

Ophthalmology Reports

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Introduction. Antiseptic drugs currently occupy an important place in the treatment and prevention of ocular infectious and inflammatory diseases. Often microorganisms are characterized not only by resistance to a single antibiotic, but also by the presence of multiple resistances, which limits the choice of an effective drug. This problem requires a detailed study and monitoring of the sensitivity of the main pathogens of ocular infections, not only to antibiotics but also to antiseptics. The aim was to study the species composition of conjunctival microflora in patients with ocular surface infection and to evaluate the antimicrobial activity of antiseptic eye drops. Materials and methods. Investigation was carried out in 20122018 in 4237 bacterial conjunctivitis patients. The sensitivity to antiseptic preparations of pathogens isolated from patients with conjunctivitis was detected. Results. 1068 strains of microorganisms isolated from the conjunctival cavity of patients were tested. Gram-positive cocci dominated among clinically significant pathogens 47.4%. Antimicrobial activity of Vitabact eye drops against gram-positive cocci was higher than that of antiseptic Okomistin. Summary. Antimicrobial activity of the studied antiseptics against gram-positive and gram-negative pathogens was different. It is necessary to conduct further research on the antimicrobial activity of antiseptic eye drops.

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Acanthamoeba keratitis. Review of literature. Case reports

Cited by 4 publications

References 12 publications

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

Combating the causative agent of amoebic keratitis, Acanthamoeba castellanii, using Padina pavonica alcoholic extract: toxicokinetic and molecular docking approaches

In vitro amoebicidal effect of Aloe vera ethanol extract and honey against Acanthamoeba spp. cysts

Monitoring the antimicrobial activity of antiseptic eye drops

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