Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures

Bouhlel, Inès; Kilani, Soumaya; Skandrani, Inès; Amar, Rebaii Ben; Nefatti, Aicha; Laporte, François; Hininger‐Favier, Isabelle; Ghedira, Kamel; Chekir‐Ghedira, Leila

doi:10.1016/j.nutres.2007.12.011

Cited by 10 publications

(6 citation statements)

References 27 publications

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“…It was suggested that quercetin and rutin may act as an antioxidant in cells by modulation of gene expression (Bouhlel et al, 2008;Granado-Serrano et al, 2010). Celik and Arinc (2010) noted that quercetin at low concentrations (below 100 µM) was able to prevent free-radical-mediated DNA damage produced by idarubicin and mitomycin C in the presence of P-450 reductase and cofactor NADPH using a cell-free agarose gel method.…”

Section: Discussionmentioning

confidence: 99%

Antimutagenic and antioxidant properties of the aqueous extracts of organic and conventional grapevineVitis labruscacv. Isabella leaves in V79 cells

Trindade

Bortolini

Costa

et al. 2016

Journal of Toxicology and Environmental Health, Part A

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Grapes are one of the most commonly consumed fruit, in both fresh and processed forms; however, a significant amount is disposed of in the environment. Searching for a use of this waste, the antigenotoxic, antimutagenic, and antioxidant activities of aqueous extracts from organic and conventional Vitis labrusca leaves were determined using V79 cells as model. The antigenotoxic activity was analyzed by the alkaline comet assay using endonuclease III and formamidopyrimidine DNA glycosylase enzymes. The antimutagenic property was assessed through the micronucleus (MN) formation, and antioxidant activities were assessed using 2',7'-dichlorodihydrofluorescin diacetate (DCFH-DA) assay and 2,2-diphenyl-1-picrylhydrazyl (DPPH(●)) radical scavenging, as well as with superoxide dismutase (SOD) and catalase (CAT) activity assays. In addition, phenolic content and ascorbic acid levels of both extracts were determined. Data showed that both organic and conventional grapevine leaves extracts possessed antigenotoxic and antimutagenic properties. The extract of organic leaves significantly reduced intracellular reactive oxygen species (ROS) levels in V79 cells, and displayed greater ability for DPPH(●) scavenging and higher SOD and CAT activities than extract from conventional leaves. Further, the extract from organic leaves contained higher phenolic and ascorbic acid concentrations. In summary, extracts from organic and conventional grape leaves induced important in vitro biological effects.

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Section: Discussionmentioning

confidence: 99%

Antimutagenic and antioxidant properties of the aqueous extracts of organic and conventional grapevineVitis labruscacv. Isabella leaves in V79 cells

Trindade

Bortolini

Costa

et al. 2016

Journal of Toxicology and Environmental Health, Part A

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show abstract

“…The inhibition of xanthine oxidase (XOD) was measured by following the decrease of uric acid absorbance at 290 nm as proposed by Bouhlel et al (2008). While the superoxide anion scavenging activity was detected spectrophotometrically with the nitrite method described by Russo et al (2005).…”

Section: Antioxidant Activitiesmentioning

confidence: 99%

Evaluation of the antimutagenic, antigenotoxic, and antioxidant activities ofEriobotrya japonicaleaves

Mokdad-Bzéouich

Kilani-Jaziri

Mustapha

et al. 2015

Pharmaceutical Biology

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Context: The leaves of Eriobotrya japonica (Thunb.) Lindl. (Rosaceae) are used in traditional medicine to treat inflammatory diseases. However, information about the antigenotoxic and antioxidant properties of its leaves remains to be elucidated. Objective: The objective of this work was to evaluate the mutagenic/antimutagenic, genotoxic/ antigenotoxic, and antioxidant potentials of aqueous and total oligomers flavonoid (TOF) extracts from E. japonica. Materials and methods: The mutagenic/antimutagenic and genotoxic/antigenotoxic potentials of extracts (50, 250, and 500 mg/plate) were evaluated, respectively, by the Ames test with 48 h incubation and the SOS chromotest test with 2 h incubation. The antioxidant capacity of these extracts (ranging from 50 to 700 mg/mL) was tested using xanthine/xanthine oxidase and the deoxyribose assays. Results: Eriobotrya japonica extracts showed neither mutagenic nor genotoxic effect. The highest protective effect against methyl methanesulfonate and 2-aminoanthracene was obtained in the presence of aqueous extract, with IC 50 values of 80 and 140 mg/plate, respectively, against S. typhimurium TA104. Moreover, this extract (500 mg/plate) was also able to reduce significantly the genotoxicity induced by nitrofurantoin and aflatoxin B1 with IC 50 values of 140 and 240 mg/assay, respectively. Likewise, aqueous and TOF extracts inhibited xanthine oxidase and superoxide anion formation with IC 50 values ranging from 45 to 95 and from 70 to 90 mg/mL, respectively. However, TOF extract is more efficient in inhibiting hydroxyl radical and chelating iron ion with IC 50 values of 140 and 400 mg/mL, respectively, when compared with the aqueous extract. Conclusion: Eriobotrya japonica prevents the genotoxicity of some carcinogenic substances probably thanks to its antioxidant capacities.

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“…1B). The various species of Acacia have low or no toxicity and may even be antigenotoxic (Bouhlel et al, 2008;Bouhlel et al, 2009). Previous studies indicated that aqueous extracts of A. aroma were neither cytotoxic nor genotoxic while ethanolic extracts showed genotoxicity at concentrations above 5 mg/mL (Mattana et al, 2012).…”

Section: Resultsmentioning

confidence: 99%

Comet assay application in evaluation the safe use of medicinal plants

Mohamed¹,

Cangiano²,

Alcaráz³

et al. 2016

Emir. J. Food Agric

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Acacia salicina extracts protect against DNA damage and mutagenesis in bacteria and human lymphoblast cell K562 cultures

Cited by 10 publications

References 27 publications

Antimutagenic and antioxidant properties of the aqueous extracts of organic and conventional grapevineVitis labruscacv. Isabella leaves in V79 cells

Antimutagenic and antioxidant properties of the aqueous extracts of organic and conventional grapevineVitis labruscacv. Isabella leaves in V79 cells

Evaluation of the antimutagenic, antigenotoxic, and antioxidant activities ofEriobotrya japonicaleaves

Comet assay application in evaluation the safe use of medicinal plants

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