Acacia jacquemontii ethyl acetate extract reduces hyperglycemia and pro-inflammatory markers while increasing endogenous antioxidant potential in alloxan-induced diabetic rats

Awan, Ambreen Mehmood; Majeed, Wafa; Muhammad, Faqir; Faisal, Muhammad Naeem

doi:10.1007/s11356-022-19493-4

Cited by 4 publications

(2 citation statements)

References 48 publications

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“…It was reported that coumarins, such as auraptene, reduced NO production and COX-2, TNF-α, IL-1β, and iNOS expression in RAW 264.7 cells ( Hsia et al, 2021 ) and the hippocampus ( Amini-Khoei et al, 2022 ). Phenolic acids, such as gallic acid, decrease NO levels and exhibit antioxidant and anti-inflammatory effects ( Awan et al, 2022 ). 5-O-Caffeoylshikimic acid suppressed not only the production of NO but also the expression of iNOS, TNF-α, and IL-1β ( Lee et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Xiongshao Zhitong Recipe Attenuates Nitroglycerin-Induced Migraine-Like Behaviors via the Inhibition of Inflammation Mediated by Nitric Oxide Synthase

et al. 2022

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Migraine is a major cause of disability worldwide, particularly in young adults and middle-aged women. Xiongshao Zhitong Recipe (XZR) is a traditional Chinese medicine prescription used for treating migraine, but its bioactive components and therapeutic mechanisms remain unclear. We aimed to confirm the therapeutic effect of XZR on migraine and to determine the possible mechanism and bioactive components of XZR. Here, a sensitive UHPLC-LTQ-Orbitrap MS assay was carried out to analyze the ingredients of XZR, and a total of 62 components were identified, including coumarins, phenolic acids, phthalides, flavonoids, and terpenoids; among them, 15 components were identified in the serum samples after XZR treatment. We established a rat model of migraine via nitroglycerin (NTG) injection. The in vivo experiments demonstrated that XZR attenuated allodynia and photophobia in rats with NTG-induced migraine, and XZR also demonstrated analgesic effects. XZR reversed the abnormal levels of nitric oxide, 5-hydroxytryptamine (5-HT), calcitonin gene-related peptide (CGRP), and substance P (SP) to normal levels. XZR also downregulated inflammatory reactions, including mast cell degranulation and serum IL-1β, IL-6, and TNF-α levels. In terms of mechanism, we revealed that XZR treated NTG-induced migraine through the inhibition of neuronal nitric oxide synthase (nNOS) and inducible nitric oxide synthase (iNOS) expression in both the trigeminal nucleus caudalis (TNC) and periaqueductal gray matter (PAG), as well as the total NOS enzyme activity, which regulated the NF-κB signaling pathway. Additionally, imperatorin and xanthotoxin, two major ingredients of XZR, showed a high binding affinity to nNOS (Gly468-Leu616). In vitro, XZR, imperatorin, and xanthotoxin inhibited the nNOS expression and the NF-κB signaling pathway in lipopolysaccharide (LPS)-stimulated PC12 cells. In conclusion, we demonstrated the therapeutic effects of XZR and provided evidence that XZR played a critical anti-inflammatory role by suppressing NOS and NF-κB signaling pathway activation. Imperatorin and xanthotoxin were potential bioactive components of XZR. The findings from this study supported that XZR was a candidate herbal drug for migraine therapy.

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Section: Discussionmentioning

confidence: 99%

Xiongshao Zhitong Recipe Attenuates Nitroglycerin-Induced Migraine-Like Behaviors via the Inhibition of Inflammation Mediated by Nitric Oxide Synthase

et al. 2022

View full text Add to dashboard Cite

show abstract

“…It was reported that coumarins, such as auraptene, reduced NO production and COX-2, TNF-α, IL-1β, and iNOS expression in RAW 264.7 cells (Hsia et al, 2021) and the hippocampus (Amini-Khoei et al, 2022). Phenolic acids, such as gallic acid, decrease NO levels and exhibit antioxidant and anti-inflammatory effects (Awan et al, 2022). 5-O-Caffeoylshikimic acid suppressed not only the production of NO but also the expression of iNOS, TNFα, and IL-1β (Lee et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Phytochemical profiling, antioxidant, and tyrosinase inhibitory potential of the Acacia cyclops trunk bark: in vitro combined with in silico approach

Kaplan,

Beyaoui,

Jlizi

et al. 2024

Biomedical Chromatography

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The aim of this study was to analyze the phytochemical profile of Acacia cyclops trunk bark ethyl acetate extract using LC–tandem mass spectrometry for the first time, along with evaluating its antioxidant and anti‐tyrosinase properties. Consequently, we determined the total phenolic and flavonoid contents of the extract under investigation and identified and quantified 19 compounds, including phenolic acids and flavonoids. In addition to assessing their antioxidant potential against DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) and ABTS (2,2′‐azino‐bis‐[3‐ethylbenzothiazoline‐6‐sulfonic] acid) assays, in vitro and in silico studies were conducted to evaluate the tyrosinase inhibitory properties of the A. cyclops extract. The ethyl acetate trunk bark extract exhibited a substantial total phenolic content and demonstrated significant antioxidant activity in terms of free radical scavenging, as well as notable tyrosinase inhibitory action (half‐maximal inhibitory concentration [IC50] = 14.08 ± 1.10 μg/mL). The substantial anti‐tyrosinase activity of the examined extract was revealed through molecular docking analysis and druglikeness prediction of the main selected compounds. The findings suggest that A. cyclops extract holds promise as a potential treatment for skin hyperpigmentation disorders.

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Acacia jacquemontii ethyl acetate extract reduces hyperglycemia and pro-inflammatory markers while increasing endogenous antioxidant potential in alloxan-induced diabetic rats

Cited by 4 publications

References 48 publications

Xiongshao Zhitong Recipe Attenuates Nitroglycerin-Induced Migraine-Like Behaviors via the Inhibition of Inflammation Mediated by Nitric Oxide Synthase

Xiongshao Zhitong Recipe Attenuates Nitroglycerin-Induced Migraine-Like Behaviors via the Inhibition of Inflammation Mediated by Nitric Oxide Synthase

DataSheet1.docx

Phytochemical profiling, antioxidant, and tyrosinase inhibitory potential of the Acacia cyclops trunk bark: in vitro combined with in silico approach

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