AC/GT and AG/CT microsatellite repeats in peach [Prunus persica (L) Batsch]: isolation, characterisation and cross-species amplification in Prunus

A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers

“…In this study marker data for 'Padre' support earlier reports of 'Mission' being the seed parent. The data are also consistent with 'Nonpareil' being one parent of 'Kanpareil', 'Solano', 'Sonora' and 'Titan' (Brooks and Olmo 1997). 'Titan's' seed parent is actually known to be 'Tardy nonpareil', a late blooming somatic mutant or ''bud-sport'' of nonpareil [only rarely are differences between somatic mutants observed with SSR data (Riaz et al 2002)].…”

“…The almond cultivars 'Aldrich', 'Butte', 'Carmel', 'Monterey' 'Norman', 'Price' and 'Thompson' have previously been reported to be chance seedling selections probably originating from 'Nonpareil' 9 'Mission' crosses (Asai et al 1996;Brooks and Olmo 1997). This preliminary characterization was based on early cross-compatibility studies (Kester et al 1994) where it was shown that most chanceselection cultivars could be grouped into four crossincompatibility groups (S 1 S 7 , S 1 S 8 , S 5 S 7 , S 5 S 8 ).…”

Significant effect of accidental pollinations on the progeny of low setting Prunus interspecific crosses

“…Genomic DNA from all the plant material was isolated according to Hormaza (2002). For the SSR analysis, the parental genotypes were initially screened with eight SSR loci previously developed in peach by Cipriani et al (1999) and Sosinski et al (2000), that have already been shown to be conserved in apricot (Hormaza, 2002) and Myrobalan (Serrano et al, 2002) genotypes: pchgms2, UDP96-003, UDP96-005, UDP96-008, UDP96-018, UDP98-405, UDP98-406 and UDP98-409. Three SSR loci (pchgms2, UDP96-003 and UDP96-008), polymorphic among the parental genotypes, were selected to confirm the hybrid origin of progeny.…”

“…However, microsatellites or simple sequence repeats (SSRs) have become the markers of choice for fingerprinting and paternity analyses in most plant species (Gupta & Varshney, 2000) due to their high polymorphism, codominancy and reproducibility. Over 100 microsatellite markers have been so far reported in peach (Cipriani et al, 1999;Testolin et al, 2000;Sosinski et al, 2000;Aranzana et al, 2002Aranzana et al, , 2003Dirlewanger et al, 2002) and many of them are transferable to other related species (Hormaza, 2002;Wünsch & Hormaza, 2002).…”

Molecular Characterization and Genetic Diversity Analysis of Rice (Oryza sativaL.) Using SSR Markers

“…In addition, SSRs often have flanking regions that are highly conserved in related species, which allows the use of the same primer pairs in related genomes (Cipriani et al 1999;Sosinski et al 2000). The SSR markers are particularly suitable for evaluating genetic diversity and relationships among plant species, populations, or individuals (Kostova et al 2006;Tu et al 2007), studying rice germplasm for either conservation or utilization (Sharma et al 2007); marker-assisted selection breeding (Perez-Sackett et al 2011;Rani and Adilakshmi 2011); cultivar identification; hybrid purity analysis and gene mapping studies (Weising et al 1997;Altaf-Khan et al 2006;Rajendrakumar et al 2009;Sarao et al 2010).…”