A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers

Dangl, Gerald S.; Yang, Judy; Golino, Deborah A.; Gradziel, Thomas M.

doi:10.1007/s10681-008-9877-0

Cited by 40 publications

(28 citation statements)

References 24 publications

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“…Accurate characterization of almond seedling cultivars and rootstocks is essential for the identification of clones that will guarantee uniformity [6] of commercial orchards.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an almond collection using morphological variables to choose superior trees

Sepahvand¹,

Khadivi²,

Momenpour³

et al. 2015

Fruits

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-Introduction. Iranian almond germplasm is regarded as one of the most diverse and valuable genetics resources for almond improvement. Materials and methods. In the present study, 155 almond genotypes were evaluated to determine the overall degree of variation and to detect superior trees. The variation was observed for traits related to phenology, morphology, yield and fruit quality. Results and discussion. Nut and kernel traits contributed most of the total variation but there were also significant differences in flowering and ripening times. The majority of important correlations were determined between the characteristics representing nut size and kernel size. Cluster and principal component analyses confirmed considerable diversity in the studied germplasm. Conclusion. Genotypes MSh11, MSh100, MSh97, MSh24 and MSh126 were the best trees in terms of flowering season, consistently high fruit set, large nut and kernel size, and low percentage of double kernels. Furthermore, genotypes MSh9 and MSh110 were very late flowering and could be useful in breeding to improve flowering season of almond.

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“…Accurate characterization of almond seedling cultivars and rootstocks is essential for the identification of clones that will guarantee uniformity [6] of commercial orchards.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an almond collection using morphological variables to choose superior trees

Sepahvand¹,

Khadivi²,

Momenpour³

et al. 2015

Fruits

View full text Add to dashboard Cite

show abstract

“…On the other hand, microsatellites, or Simple Sequence Repeats (SSRs), represent reliable, robust and consistent genotypic identifiers (Gupta et al 1996; Morgante and Olivieri 1993), and have been used for a number of crops, including polyploids (Alba et al 2009;Arnau et al 2003;Ashley et al 2003;Buteler et al 1999;Dangl et al 2001Dangl et al , 2005Dangl et al , 2009Guilford et al 1997;Lian et al 2001). These markers rely on the detection of rapidly evolving polynucleotide repeats that exhibit great variation, even between closely related accessions.…”

Section: Introductionmentioning

confidence: 99%

Implementation of simple sequence repeat markers to genotype Florida strawberry varieties

et al. 2010

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Simple sequence repeats (SSRs or microsatellites) are frequently used as a robust, rapid, and relatively inexpensive means of genotyping. Recent reports in cultivated strawberry (Fragaria 9 ananassa) have identified a widely applicable set of SSRs that permits discrimination of closely-related genotypes. In the present study this same set of SSRs is analyzed in cultivars commonly grown in the state of Florida, as well as advanced breeding selections from the University of Florida program. Nine primer pairs have been used to produce discrete SSR patterns for all lines tested, including diagnostic sets that distinguish between closely-related cultivars and/or breeding selections. A comparison of markers to known pedigrees is presented, along with analysis of relative relatedness between lines studied. We also detail important technical considerations for transferability and limitations of these technologies between laboratories. The resulting genotype data for cultivars commonly used in Florida are accessible as supplemental data in graphic format and allow comparison with other cultivars. Users can thus select the most diagnostic SSRs to test the lines in question. These resources provide new tools for breeders and nurseries to authenticate genotypes, follow inheritance patterns and enforce patent protection. As important, this report underscores the strengths and weaknesses of applying the original methodologies to a different plant population in independent laboratories.

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“…This information is very relevant to apple breeders for protecting plant breeders' rights and for planning breeding programs. SSR markers have been widely used for cultivar identification of many fruit tree species (Dangl et al 2009;Sawamura et al 2008;This et al 2004;Yamamoto et al 2003).…”

Section: Introductionmentioning

confidence: 99%

“…However, this marker set was not optimized for cultivar identification because it comprised numerous markers. Seventy-five SSRs are too many for cultivar identification when compared to the number of markers used for other fruit trees such as 18 SSRs for pears (Sawamura et al 2008), 6 SSRs for grapevines (This et al 2004), 12 SSRs for almonds (Dangl et al 2009), and 10 SSRs for peaches (Yamamoto et al 2003). Therefore, a standard marker set for characterizing the genus Malus needs to be developed.…”

Section: Introductionmentioning

confidence: 99%

A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers

et al. 2010

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Differentiation of cultivars with simple sequence repeat (SSR) markers is a very useful technique for the true-to-type characterization of cultivars and clarification of parent-offspring relationships. We developed an SSR marker set for cultivar identification comprising 15 markers that were screened from 46 previously published SSRs. This marker set could be used for apple varieties including Malus 9 domestica and/or other Malus species. These SSRs successfully characterized 95 apples, including the leading and major founding cultivars used worldwide for modern apple breeding. Therefore, this marker set could be applied to almost all apple cultivars. We also analyzed the parentoffspring relationships of 69 cultivars by considering allele transmissions. This analysis revealed the true parentage of the following seven cultivars: 'Kizashi', 'Chinatsu', 'Honey Queen', 'Haruka', 'Seirin', 'Ozenokurenai', and Morioka #48. This analysis also revealed a parentage discrepancy for 'Hacnine'. From the parent-offspring analysis, two microsatellite mutation events at alleles inherited from pollen parents were observed.

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A practical method for almond cultivar identification and parental analysis using simple sequence repeat markers

Cited by 40 publications

References 24 publications

Evaluation of an almond collection using morphological variables to choose superior trees

Evaluation of an almond collection using morphological variables to choose superior trees

Implementation of simple sequence repeat markers to genotype Florida strawberry varieties

A practical method for apple cultivar identification and parent-offspring analysis using simple sequence repeat markers

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