Abundant Microorganism in Soil1

Casida, L. E.

doi:10.1128/aem.13.3.327-334.1965

Cited by 45 publications

(13 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Natural environments, such as deep ocean waters or soil, are often deficient in growth-promoting nutrients, so bacteria became reduced in size as a physiological survival response to starvation (2,5,10,16,17). Conditions deep within the rock strata may similarly produce microorganisms that are reduced in size due to carbon deprivation.…”

Section: Discujssionmentioning

confidence: 99%

Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery

Lappin‐Scott

Cusack

Costerton

1988

Appl Environ Microbiol

View full text Add to dashboard Cite

Klebsiella pneumoniae, which was reduced in size (0.25 by 0.5 ,um) by carbon deprivation, was injected into a series of sandstone cores and subjected to separate treatments. Scanning electron microscopy of 400-mD cores showed these small starved cells in nearly every core section. The cells were a mixture of small rods and cocci with little or no biofilm production. Continuous or dose stimulation with sodium citrate allowed the cells to grow throughout the sandstone and completely plug the length of the core. The resuscitated cells were larger than the starved cells (up to 1.7 ,um) and were encased in glycocalyx. Scanning electron microscopic results of resuscitation in situ with half-strength brain heart infusion broth showed that a shallow "skin" plug of cells formed at the core inlet and that fewer cells were located in the lower sections. Starved cells also penetrated 200-mD cores and were successfully resuscitated in situ with sodium citrate, so that the entire core was plugged. Nutrient resuscitation of injected starved cells to produce full-size cells which grow and block the rock pores may be successfully applied to selective plugging and may effectively increase oil recovery.

show abstract

Section: Discujssionmentioning

confidence: 99%

Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery

Lappin‐Scott

Cusack

Costerton

1988

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Bacteria from marine environments have been shown to be small enough to pass through 0.45 pm membrane filters (Tabor et al 1981;Torrella & Morita 1981;MacDonell & Hood 1982). In soil environments, bacteria less than 0.3 pm (Bae et al 1972) or 0.5 pm (Casida 1965) in diameter have been observed by electron microscopy.…”

mentioning

confidence: 99%

Starvation and nutrient resuscitation of Klebsiella pneumoniae isolated from oil well waters

Lappin‐Scott

Cusack

MacLeod

et al. 1988

Journal of Applied Bacteriology

View full text Add to dashboard Cite

Klebsiella pneumoniae isolated from oil well waters reduced in size in response to nutrient starvation. The cells remained viable during starvation and later were able to grow rapidly when stimulated by nutrients. The heterotrophic potential, culture absorbance and extracellular polysaccharide production decreased during cell starvation whereas an initial increase in colony-forming units was observed on agar plates. Transmission electron microscopy (TEM) after 24 d revealed that the cells had changed to small rods or cocci between 0.5 by 0.25 micron and 0.87 by 0.55 micron. When transferred to half-strength brain heart infusion medium, TEM showed cell division and rod-shaped cells after 45 min and full resuscitation within 4 h. Cell response was much slower in sodium citrate medium and resuscitation took 8 h.

show abstract

“…It produces a branched mycelium that ultimately fragments into coccoid and diphtheroid forms. Special techniques are required for its isolation from soil (2,7,12), and there are no methods available for enumerating it from soil. Nevertheless, the A. ramosus numbers in soil are greater than the total numbers for all soil bacteria that are countable or isolatable by the usual bacteriological t Paper no.…”

mentioning

confidence: 99%

Interaction of Agromyces ramosus with Other Bacteria in Soil

1983

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

Agromyces ramosus occurs in very high numbers in most soils and, based on studies of laboratory isolates, does not require host cells for growth. Nevertheless, it attacked and destroyed most of the gram-positive and gram-negative bacterial species tested as possible host organisms. A. ramosus also attacked and destroyed Saccharomyces cerevisiae. The possibility of attack on fungi was unclear. Among the bacteria serving as hosts were the important soil species Azotobacter vinelandii, Rhizobium leguminosarum, Rhizobium meliloti , and Agrobacterium tumefaciens. Dead cells were not attacked. A. vinelandii cysts were attacked but left unharmed. To some extent, A. vinelandii seemed to survive this attack by encysting. Attack by A. ramosus occurred in natural soil and over a broad range of nutritional levels in laboratory media. The attack did not seem to be a means for obtaining an increased supply of commonly available nutrients. Instead, it seemed to be a means of obtaining something produced, perhaps in small amounts, by a variety of organisms, but not by all organisms. Several types of culture filtrates were tested for activity. The filtrates neither stimulated nor inhibited the growth of A. ramosus or the host organisms. The availability of catalase activity in host organisms did not seem to be involved. It is not known whether the attack by Agromyces ramosus in soil can be manipulated to cause a decrease in numbers of Agrobacterium tumefaciens or other pathogens without simultaneously depressing the numbers of beneficial organisms in this habitat.

show abstract

Abundant Microorganism in Soil1

Cited by 45 publications

References 6 publications

Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery

Nutrient Resuscitation and Growth of Starved Cells in Sandstone Cores: a Novel Approach to Enhanced Oil Recovery

Starvation and nutrient resuscitation of Klebsiella pneumoniae isolated from oil well waters

Interaction of Agromyces ramosus with Other Bacteria in Soil

Contact Info

Product

Resources

About