Abundance, diversity, and regulation of mRNA sequence sets in soybean embryogenesis

Goldberg, Robert B.; Hoschek, Gisela; Tam, Susan H.; Ditta, Gary S.; Breidenbach, R. W.

doi:10.1016/0012-1606(81)90467-x

Cited by 160 publications

(90 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5A), a value close to that which we obtained more than a quarter of a century ago using Rot curve hybridization technology (Goldberg et al, 1981b(Goldberg et al, , 1989. A smaller number of diverse mRNAs were found to be present in individual soybean globularstage seed regions.…”

Section: Using Soybean To Identify Genes Required To Make a Seedsupporting

confidence: 79%

“…During this same period, our laboratory used RNAexcess DNA-RNA hybridization experiments to show that approximately 14,000 to 18,000 diverse mRNAs are present in soybean embryos at different developmental stages (Goldberg et al, 1981b(Goldberg et al, , 1989. We also demonstrated that most diverse mRNA species are present throughout seed development, but that small numbers of mRNAs, including those encoding storage proteins, are regulated quantitatively at specific developmental stages (Goldberg et al, 1981a).…”

Section: Legumes Have Been Used To Study Seed Development For More Thmentioning

confidence: 93%

See 1 more Smart Citation

Using Genomics to Study Legume Seed Development

Wagmaister²,

Kawashima³

et al. 2007

Plant Physiology

Self Cite

148

157

View full text Add to dashboard Cite

Section: Using Soybean To Identify Genes Required To Make a Seedsupporting

confidence: 79%

Section: Legumes Have Been Used To Study Seed Development For More Thmentioning

confidence: 93%

Using Genomics to Study Legume Seed Development

Wagmaister²,

Kawashima³

et al. 2007

Plant Physiology

Self Cite

148

157

View full text Add to dashboard Cite

“…The mRNAs for SBL and SBTI along with those fo; soybean seed proteins of higher abundance reflect a small developmentally regulated gene set. This small number (7-10) of abundant RNAs comprises 50%o of the midmaturation stage mRNA mass and is encoded by low frequency repetitive DNAs (9). These mRNAs increase in quantity during early maturation and decline dramatically during late maturation.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Characterization of Messenger RNAs for Seed Lectin and Kunitz Trypsin Inhibitor in Soybeans

Vodkin¹

1981

Plant Physiol.

View full text Add to dashboard Cite

The mRNAs for seed lectin and Kuntz tpin inbitor of In addition to substantial biochemical data for these two seed proteins, lectin and trypsin inhibitor have been defined by classical genetic analyses and soybean varieties which lack detectable SBL or SBTI protein have been found (21,22,24). It is the aim of this report and other studies in progress to determine what molecular defects cause the inherited absence of SBL or SBTI protein in certain soybean varieties. Such knowledge will help elucidate the normal functioning and expression of plant genes. As an initial step toward this objective, I have developed modifications to a polysome immunoadsorption procedure which have allowed the isolation of highly enriched mRNAs for lectin and Kunitz trypsin inhibitor. A preliminary report has been published (33). The selected mRNAs were analyzed by their activities in a rabbit reticulocyte cell free translation system and by hybridization of labeled SBL or SBTI cDNA to gel blots of total poly(A) RNA.

show abstract

“…The reduction in the number of seed mRNAs detected during late development (MG-PMG) is probably due to mRNA turnover resulting from the general shutdown in transcriptional processes as the seed enters dormancy (48). More than a generation ago, we (49) and others (50), used RNA/cDNA hybridization experiments to investigate mRNA populations during seed development. Our conclusions from that era, using primitive technology that provided the foundation for questions being addressed currently with sophisticated genomics approaches, are in remarkable agreement with those reported here.…”

Section: Discussionmentioning

confidence: 99%

Global analysis of gene activity duringArabidopsisseed development and identification of seed-specific transcription factors

Le¹,

Cheng²,

Bui³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

507

493

View full text Add to dashboard Cite

Most of the transcription factors (TFs) responsible for controlling seed development are not yet known. To identify TF genes expressed at specific stages of seed development, including those unique to seeds, we used Affymetrix GeneChips to profile Arabidopsis genes active in seeds from fertilization through maturation and at other times of the plant life cycle. Seed gene sets were compared with those expressed in prefertilization ovules, germinating seedlings, and leaves, roots, stems, and floral buds of the mature plant. Most genes active in seeds are shared by all stages of seed development, although significant quantitative changes in gene activity occur. Each stage of seed development has a small gene set that is either specific at the level of the GeneChip or up-regulated with respect to genes active at other stages, including those that encode TFs. We identified 289 seed-specific genes, including 48 that encode TFs. Seven of the seed-specific TF genes are known regulators of seed development and include the LEAFY COTYLEDON ( LEC ) genes LEC1, LEC1-LIKE, LEC2 , and FUS3 . The rest represent different classes of TFs with unknown roles in seed development. Promoter-β -glucuronidase ( GUS ) fusion experiments and seed mRNA localization GeneChip datasets showed that the seed-specific TF genes are active in different compartments and tissues of the seed at unique times of development. Collectively, these seed-specific TF genes should facilitate the identification of regulatory networks that are important for programming seed development.

show abstract

Abundance, diversity, and regulation of mRNA sequence sets in soybean embryogenesis

Cited by 160 publications

References 58 publications

Using Genomics to Study Legume Seed Development

Using Genomics to Study Legume Seed Development

Isolation and Characterization of Messenger RNAs for Seed Lectin and Kunitz Trypsin Inhibitor in Soybeans

Global analysis of gene activity duringArabidopsisseed development and identification of seed-specific transcription factors

Contact Info

Product

Resources

About