ABTS assay of phenol oxidase activity in soil

Effects of soil properties and management on the activity of soil organic matter transforming enzymes and the quantification of soil-bound and free activity

“…Despite the fact that ligninolytic enzymes are usually more mobile than polysaccharide hydrolases , their total activity in soils is also difficult to assess. Although a method for direct measurement of laccase was proposed, its use in soils is limited due to its colorimetric nature (Floch et al 2007). In addition, direct assays of these enzymes are also complicated by the fact that they act on humic substances abundant in soils and this results in competitive inhibition of the assay.…”

Diversity of Two-Domain Laccase-Like Multicopper Oxidase Genes in Streptomyces spp.: Identification of Genes Potentially Involved in Extracellular Activities and Lignocellulose Degradation during Composting of Agricultural Waste

“…Phenol oxidase activity was estimated using the substrate 2,2=-azino-bis-(3-ethylbenzothiazoline-6-sulfononic acid) diammonium salt (ABTS) at pH 4.5 or L-3,4-dihydroxyphenylalanine (L-DOPA) at pH 6.0 and 8.0 (12,23,24). Compost samples (10 g) were shaken in flasks with 90 ml of sterile 0.9% NaCl solution at 25°C for 1 h. The resulting suspension was used for measuring the phenol oxidase activity.…”

An Efficient Method for Qualitative Screening of Ligninolytic Enzyme Potential of Ganoderma lucidum

“…This confirmation is supposed to be a baseline for production of lignin modifying enzymes before their optimization and application (Wunch et al, 1997;Alfarra et al, 2013). The commonly used indicator pointers are guaiacol, 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), syringaldazine, tannic acid, Azure B, Remazol Brilliant Blue-R (RBBR), 2,4-dichlorophenol (2,4-DCP) (Coll 1993;D'Souza et al, 1999;Floch et al, 2007;Alfarra et al, 2013;Casciello et al, 2017). Positive results have been experiential through the different variations which came into view on the diverse solid medium.…”