ABSORPTION SPECTRA OF LEAVES CORRECTED FOR SCATTERING and DISTRIBUTIONAL ERROR: A RADIATIVE TRANSFER and ABSORPTION STATISTICS TREATMENT

Fukshansky, Leonid; Remisowsky, Alexander Martinez v.; McClendon, John H.; Ritterbusch, A.; Richter, T.; Mohr, Hans

doi:10.1111/j.1751-1097.1993.tb02332.x

Cited by 96 publications

(50 citation statements)

References 23 publications

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“…The differences observed between species may be related to differences in the distribution of chlorophyll in tree leaves as a result of the structural organization of chlorophyll molecules in chloroplasts, chloroplasts in cells, and cells in leaves (Fukshansky et al, 1993). It is generally accepted that with increasing heterogeneity of chlorophyll distribution inside a leaf, the absorption of a given amount of chlorophyll decreases (Uddling et al, 2007) and the relationship between SPAD and chlorophyll content deviates more from linearity.…”

Section: Discussionmentioning

confidence: 99%

Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

et al. 2010

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Abstract• Chlorophyll meters such as the SPAD-502 offer a simple, inexpensive and rapid method to estimate foliar chlorophyll content. However, values provided by SPAD-502 are unitless and require empirical calibrations between SPAD units and extracted chlorophyll values.• Leaves of 13 tree species from the tropical rain forest in French Guiana were sampled to select the most appropriate calibration model among the often-used linear, polynomial and exponential models, in addition to a novel homographic model that has a natural asymptote.• The homographic model best accurately predicted total chlorophyll content (μg cm −2 ) from SPAD units (R 2 = 0.89). Interspecific differences in the homographic model parameters explain less than 7% of the variation in chlorophyll content in our data set.• The utility of the general homographic model for a variety of research and management applications clearly outweighs the slight loss of model accuracy due to the abandon of the species' effect.

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Section: Discussionmentioning

confidence: 99%

Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

et al. 2010

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“…So, it is clear that the relationship between Chl content and Chl meter-reading does not exhibit a similar pattern for all species. These differences among species may be related to the non-uniform distribution of Chl in leaves as an effect of the clustered structural organization of Chl molecules in chloroplasts, chloroplasts in cells, and cells in leaves (Fukshansky et al 1993). The curvilinear relationship suggested that CCM-200 underestimated total Chl at high concentrations (Fig.…”

Section: Discussionmentioning

confidence: 99%

Non-destructive estimation of chlorophyll and nitrogen content in leaf ofRosa damascena(Mill)

Singh¹,

Prasad²

2012

Soil Science and Plant Nutrition

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Chlorophyll (Chl) and nitrogen (N) status of leaves provide valuable information about the physiological condition of plants. The conventional methods for measuring Chl and N contents in leaves are destructive, costly, time-consuming, and do not allow repetitive measurement of the same sample. The Damask Rose (Rosa damascena Mill) is an important aromatic crop in the western Himalaya region in India. Generally, flower yield and oil yield of the Damask rose are correlated with nitrogen, phosphorus, and potassium (NPK) levels in the leaf at the bud development stage. The dynamics of N within the rose plant have not been reported clearly. Thus, there is a pressing need for non-destructive techniques to estimate Chl and N content in the leaf of the Damask rose. Our objective was to establish an appropriate mathematical relationship between the Chl content index (CCI) value and the total Chl/N contents for non-destructive estimation of total Chl and N in the leaf of the Damask rose. The regression models were developed with destructively measured parameters (total Chl and N) as the dependent variable and a parameter derived from CCM-200 as the independent variable (CCI). We found that polynomial regression models are suitable for non-destructive estimation of total Chl, and the model predicted values were very close to traditionally measured values with a root mean square prediction error (RMSEp) less than 0.20 mg g À1 of Chl. In the case of N estimation, a power regression model was appropriate with lowest Akaike's information criteria (AIC) and root mean square validated error (RMSEv) value. Significant correlations (P 0.001) were observed between traditionally measured values and our model predicted values in both cases.

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“…The new measuring system opens the way to a profound assessment of the specific contributions of various leaf regions to the overall photosynthetic activity. This may be considered an important step forward towards an integrative modelling of leaf photosynthesis, the theoretical basis for which has been advanced by Fukshansky and co-workers Fukshansky et al 1992;Martinez v. Remisowsky et al 1992). Fiberoptic microprobe measurements can provide information on the vertical gradient of photosynthetic activity within a leaf, while fluorescence image analysis provides information on the lateral distribution, as viewed from the surface (Myers et al 1994;Siebke and Weis 1995;Genty and Meyer 1995).…”

Section: Discussionmentioning

confidence: 99%

Measurement of chlorophyll fluorescence within leaves using a modified PAM Fluorometer with a fiber-optic microprobe

et al. 1996

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By using a fiber-optic microprobe in combination with a modified PAM Fluorometer, chlorophyll fluorescence yield was measured within leaves with spatial resolution of approximately 20 μm. The new system employs a miniature photomultiplier for detection of the pulse-modulated fluorescence signal received by the 20 μm fiber tip. The obtained signal/noise ratio qualifies for recordings of fluorescence induction kinetics (Kautsky effect), fluorescence quenching by the saturation pulse method and determination of quantum yield of energy conversion at Photosystem II at different sites within a leaf. Examples of the system performance and of practical applications are given. It is demonstrated that the fluorescence rise kinetics are distinctly faster when chloroplasts within the spongy mesophyll are illuminated as compared to palisade chloroplasts. Photoinhibition is shown to affect primarily the quantum yield of the palisade chloroplasts when excessive illumination is applied from the adaxial leaf side. The new system is envisaged to be used in combination with light measurements within leaves for an assessment of the specific contributions of different leaf regions to overall photosynthetic activity and for an integrative modelling of leaf photosynthesis.

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ABSORPTION SPECTRA OF LEAVES CORRECTED FOR SCATTERING and DISTRIBUTIONAL ERROR: A RADIATIVE TRANSFER and ABSORPTION STATISTICS TREATMENT

Cited by 96 publications

References 23 publications

Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

Non-destructive estimation of chlorophyll and nitrogen content in leaf ofRosa damascena(Mill)

Measurement of chlorophyll fluorescence within leaves using a modified PAM Fluorometer with a fiber-optic microprobe

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