Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

Coste, Sabrina; Baraloto, Christopher; Leroy, Céline; Marcon, Éric; Renaud, Amélie; Richardson, Andrew D.; Roggy, Jean-Christophe; Schimann, Heidy; Uddling, Johan; Hérault, Bruno

doi:10.1051/forest/2010020

Cited by 173 publications

(143 citation statements)

References 20 publications

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“…The relationship depends on leaf structure factors such as leaf thickness, leaf mass per area (LMA) [28], and the proportion of vascular tissue [59], characteristics known to vary among species. Equations reported by Coste et al [28], Cerovic et al [59], and Marenco et al [60] have general applicability as they are estimated from different species covering a range of leaf characteristics. For the particular case of mangroves, the only equation reported in the literature is that of Connelly [30], established for the red mangrove (R. mangle).…”

Section: Spad Calibrationmentioning

confidence: 99%

“…A more practical technique that complements the aforementioned approach consists on the use of portable Chl meters such as the Opti-Sciences CCM-200 Chlorophyll Content Meter (CCM-200) and Minolta SPAD-502 Chlorophyll Meter (SPAD-502). Portable Chl meters have been used extensively in precision agriculture and have been tested on a variety of tree species [25][26][27][28][29]. To our knowledge, the first documented example of the use a portable Chl meter in mangrove species is Connelly [30].…”

Section: Introductionmentioning

confidence: 99%

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Spatiotemporal Variation in Mangrove Chlorophyll Concentration Using Landsat 8

et al. 2015

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There is a need to develop indicators of mangrove condition using remotely sensed data. However, remote estimation of leaf and canopy biochemical properties and vegetation condition remains challenging. In this paper, we (i) tested the performance of selected hyperspectral and broad band indices to predict chlorophyll concentration (CC) on mangrove leaves and (ii) showed the potential of Landsat 8 for estimation of mangrove CC at the landscape level. Relative leaf CC and leaf spectral response were measured at 12 Elementary Sampling Units (ESU) distributed along the northwest coast of the Yucatan Peninsula, Mexico. Linear regression models and coefficients of determination were computed to measure the association between CC and spectral response. At leaf level, the narrow band indices with the largest correlation with CC were Vogelmann indices and the MTCI (R 2 > 0.5). Indices with spectral bands around the red edge (705-753 nm) were more OPEN ACCESSRemote Sens. 2015, 7 14531 sensitive to mangrove leaf CC. At the ESU level Landsat 8 NDVI green, which uses the green band in its formulation explained most of the variation in CC (R 2 > 0.8). Accuracy assessment between estimated CC and observed CC using the leave-one-out cross-validation (LOOCV) method yielded a root mean squared error (RMSE) = 15 μg·cm −2 , and R 2 = 0.703. CC maps showing the spatiotemporal variation of CC at landscape scale were created using the linear model. Our results indicate that Landsat 8 NDVI green can be employed to estimate CC in large mangrove areas where ground networks cannot be applied, and mapping techniques based on satellite data, are necessary. Furthermore, using upcoming technologies that will include two bands around the red edge such as Sentinel 2 will improve mangrove monitoring at higher spatial and temporal resolutions.

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Section: Spad Calibrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Variation in Mangrove Chlorophyll Concentration Using Landsat 8

et al. 2015

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“…We sampled individuals other than those censused for herbivory and leaf production because our methods included destructive sampling. On three leaves per individual, leaf chlorophyll content was estimated using three values from a Minolta SPAD 502DL meter (Spectrum Technologies, Plainfield, Illinois, USA) with calibrations after Coste et al (2010); leaf thickness was measured as the mean of three measurements with a digital micrometer (Mitutoyo Instruments, Singapore), and leaf toughness was measured as the average of three punch tests with a Chatillon penetrometer (Ametek, Largo, Florida, USA). The leaves were scanned using a portable scanner (LiDE 60; Canon, Lake Success, New York, USA), and their area was determined by image analyses with Winfolia software (Regent Instruments, Toronto, Canada).…”

Section: Leaf Functional Traitsmentioning

confidence: 99%

Herbivory, growth rates, and habitat specialization in tropical tree lineages: implications for Amazonian beta‐diversity

Lamarre

Baraloto

Fortunel

et al. 2012

Ecology

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Abstract. Tropical plant diversity is extraordinarily high at both local and regional scales. Many studies have demonstrated that natural enemies maintain local diversity via negative density dependence, but we know little about how natural enemies influence beta-diversity across habitats and/or regions. One way herbivores could influence plant beta-diversity is by driving allocation trade-offs that promote habitat specialization across resource gradients. We therefore predicted that increasing resource availability should be accompanied by increasing herbivory rates and decreasing plant allocation to defense. Second, relative abundances within plant lineages are predicted to reflect patterns of habitat specialization and allocation tradeoffs. A phylogenetic context is vital not only to compare homologous plant traits (including defense strategies) across habitat types, but also to connect evolutionary trade-offs to patterns of species diversification in each phylogenetic lineage.We tested these predictions for trees in white-sand, clay terra firme, and seasonally flooded forests in Peru and French Guiana that represent the range of soil fertility, forest structure, and floristic compositions found throughout the Amazon region. We established 74 0.5-ha plots in these habitats and sampled all trees. Within 12 representative plots we marked newly expanding leaves of 394 saplings representing 68 species, including the most abundant species in each plot in addition to species from five focal lineages: Swartzia and Inga (Fabaceae), Protieae (Burseracaeae), Bombacoideae (Malvaceae), and Micropholis (Sapotaceae). We measured total leaf production rates for each sapling and calculated relative herbivory impact as the ratio between herbivory rate and leaf production rate.Herbivory rates averaged 2.1% per month, did not correlate with leaf production rate, and were similar across habitats. Relative herbivore impacts exceeded leaf production rates for most species. Leaf production rate averaged 2.8%, was significantly higher in seasonally flooded forests than the other two habitats, and exhibited significant correlations with specific leaf area. Species with high herbivory rates exhibited significantly lower relative abundances in terra firme forests. Overall, focal species within lineages present contrasting patterns regarding their herbivory rates and leaf production rate within habitats. These results highlight why a lineage-based approach is necessary when attempting to connect hypotheses regarding evolutionary trade-offs to community assembly patterns.

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“…An imbalance between biosynthesis and catabolic turnover of green pigments in plant tissues indicates profound inhibition of photosynthesis process (Heng-Moss et al, 2003;Smith et al, 2005;Botha et al, 2006;Diaz-Montano et al, 2007;Goławska et al, 2010). Portable leaf-clip-type chlorophyll meters perform rapid, repetitive and nondestructive estimates of Chl concentrations in living plants (Hawkins et al, 2009;Jinwen et al, 2009;Coste et al, 2010;Gholizadeh et al, 2011;Shrestha et al, 2012;Ghosh et al, 2013). The use of these reliable and advanced instruments for measurement of green pigment content is not restricted to controlled laboratory bioassays but can be extended to experiments in the plants' natural environment (Uddling et al, 2007;Ruiz-Espinoza et al, 2010;Neto et al, 2011;Cerovic et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

Sytykiewicz¹,

Czerniewicz²,

Sprawka³

et al. 2013

Acta Biologica Cracoviensia Series Botanica

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We measured the total chlorophyll (Chl a+b) content in seedling leaves of fifteen maize cultivars infested by two studied aphid species (oligophagous Rhopalosiphum padi L., monophagous Sitobion avenae F.) 7 and 14 days after the beginning of infestation, using a SPAD-502 chlorophyll meter. Chlorophyll loss was more severe in R. padi-infested than in S. avenae-infested plants. Chlorophyll depletion was greater after long-term (14 days) than after short-term aphid infestation in the investigated host systems. Seedlings of Złota Karłowa and Tasty Sweet were more damaged by aphid feeding; Ambrozja and Płomyk plants were less damaged by aphid feeding.K Ke ey y w wo or rd ds s: : Total chlorophyll (Chl a+b) content, SPAD units, maize, Rhopalosiphum padi, Sitobion avenae.

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Assessing foliar chlorophyll contents with the SPAD-502 chlorophyll meter: a calibration test with thirteen tree species of tropical rainforest in French Guiana

Cited by 173 publications

References 20 publications

Spatiotemporal Variation in Mangrove Chlorophyll Concentration Using Landsat 8

Spatiotemporal Variation in Mangrove Chlorophyll Concentration Using Landsat 8

Herbivory, growth rates, and habitat specialization in tropical tree lineages: implications for Amazonian beta‐diversity

Chlorophyll Content of Aphid-Infested Seedling Leaves of Fifteen Maize Genotypes

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