Absorption of foliar-applied Zn in sunflower (<i>Helianthus annuus</i>): importance of the cuticle, stomata and trichomes

Li, Cui; Wang, Peng; Ent, Antony van der; Cheng, Ming; Jiang, Haibo; Read, Thea L.; Lombi, Enzo; Tang, Caixian; Jonge, Martin D. de; Menzies, Neal W.; Kopittke, Peter M.

doi:10.1093/aob/mcy135

Cited by 99 publications

(81 citation statements)

References 53 publications

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“…These findings indicate that nano-ZnO was likely absorbed in the same form as ZnSO4 -soluble Zn ions rather than as intact ZnO particles. This is in agreement with previous studies, including a similar studies of Li et al (2018) in soybean and Alexander et al (2016) found that absorption of insoluble foliar applied nutrients was limited (even for nano-scale particles). Therefore, solubility is an important factor to consider when designing foliar fertilisers.…”

Section: Comparing the Absorption Of Nano-zno And Znso4supporting

confidence: 94%

“…100 µm that had the highest Zn concentrations. These corresponded to the non-glandular trichomes of sunflower in which foliar-applied Zn accumulated to high concentrations (Li et al 2018).…”

Section: Distribution Of Zn In Yfels Analysed Using µ-Xrfmentioning

confidence: 99%

“…Two types of Zn fertilisers were prepared at 1000 mg Zn L -1 (15.4 mM), being ZnSO4.7H2O (pH 5.2) and nano-ZnO (ca. 30 nm diameter, pH 7.1) [see Li et al (2018) for scanning electron micrograph and the dynamic light scattering results of the nano-ZnO].…”

Section: Laterally-resolved Examination Of Zn Distribution In Leaf Timentioning

confidence: 99%

“…However, it is known that trichomes are highly diverse (Ohrui et al, 2007, Werker, 2000, and it is likely that not all plant species would have trichomes that are important for foliar nutrient absorption. For example, Li et al (2018) found that trichomes of tomato (Solanum lycopersicum) and soybean (Glycine max)…”

Section: Role Of Trichomes In Foliar Zn Absorptionmentioning

confidence: 99%

“…In particular, it is important to understand whether the rapid movement of Zn into the NGT is a specific absorption mechanism, or whether it occurs indirectly due to absorption of water as a drought stress mechanism. In a related manner, it is necessary to understand differences among plant species, including why the trichomes of some plant species do not absorb foliar-applied nutrients (Li et al, 2018) but others do. Finally, it would also be useful to assess the impact of growth conditions (such as water stress) on the absorption of nutrients by trichomes.…”

Section: Role Of Trichomes In Foliar Zn Absorptionmentioning

confidence: 99%

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Absorption and translocation of Zn foliar fertilisers

Cui¹

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Crop Zn deficiency is associated with low Zn availability in soils and is a widespread problem, also resulting in human Zn deficiency. Foliar Zn fertilisation has been shown to be an efficient approach for overcoming this problem. However, the mechanisms by which Zn moves across the leaf surface (penetration across the cuticle and epidermal cells and into the underlying tissues) and is subsequently translocated remain unclear, with this being the main focus of the present research. Specifically, using ZnSO4 and nano-ZnO, the roles of leaf cuticle, trichomes, and stomata in foliar Zn absorption were examined, with the distribution and the speciation of the absorbed Zn in the leaves determined. In addition, the influence of plant Zn status on foliar Zn absorption was also examined. The first experiment aimed to obtain a general understanding of the effects of leaf properties on the foliar absorption of Zn. Methyl jasmonate (MeJA) (0, 0.1, 0.5, 1, and 2.5 mM) was applied to leaves of soybean (Glycine max), sunflower (Helianthus annuus), and tomato (Solanum lycopersicum) to alter leaf properties. It was found for all three plant species that treating of MeJA caused substantial increases (1.3-3.5-times higher) in leaf trichome density, and the hydrophobicity of the adaxial leaf surface. The changes in stomatal density and leaf cuticle thickness varied with plant species and MeJA concentration. Based upon these results, the second experiment used 0 (control) and 1 mM MeJA treatments to examine the effects of changes in leaf properties on the absorption of foliar-applied Zn, Mn, and Fe, using synchrotron-based X-ray fluorescence microscopy (µ-XRF) to examine the distribution of elements in situ within hydrated leaves. Interestingly, foliar absorption of Zn, Mn, and Fe increased up to 3-to 5-fold in sunflower leaves treated with 1 mM MeJA, but decreased by 0.5-to 0.9-fold in leaves of tomato treated with 1 mM MeJA. It was found that these changes were related to the thickness of the cuticle and epidermal cell wall, suggesting that the cuticle is important for absorption of foliar-applied nutrients. However, subsequent translocation of the absorbed Zn, Mn, and Fe within the leaf tissues was limited (moving < 1.3 mm in 6 h) irrespective of the plant species and treatment. Of the three nutrients, Zn was translocated a greater distance within the vein than in the interveinal tissues (being translocated ca. twofold further). The third experiment gave specific consideration to the role of trichomes in foliar Zn absorption. Using µ-XRF for the in situ analyses of nutrient distribution in leaves of soybean and tomato, it was found that upon the foliar-application of ZnSO4, Zn accumulated in some spectroscopy (XAS) analyses showed that the Zn in the leaves following foliar application was mostly present as Zn phytate (37-53%) in Zn sufficient leaves, but as Zn phosphate (about 55%) in Zn deficient leaves. Across several of the experiments described above, the foliar absorption of ZnSO4 and nano-ZnO were compared. It was found tha...

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Section: Comparing the Absorption Of Nano-zno And Znso4supporting

confidence: 94%

Section: Distribution Of Zn In Yfels Analysed Using µ-Xrfmentioning

confidence: 99%

Section: Laterally-resolved Examination Of Zn Distribution In Leaf Timentioning

confidence: 99%

Section: Role Of Trichomes In Foliar Zn Absorptionmentioning

confidence: 99%

Section: Role Of Trichomes In Foliar Zn Absorptionmentioning

confidence: 99%

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Absorption and translocation of Zn foliar fertilisers

Cui¹

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Tracking Metal Ions in Biology UsingX‐Ray Methods

James

Ent

Harris

2019

Encyclopedia of Inorganic and Bioinorganic Chemistry

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In this article, we describe the current state‐of‐play and potential future developments for a set of experimental methods that rely on X‐rays to probe metal ions in biological systems, especially X‐ray fluorescence imaging (XFI) and X‐ray absorption spectroscopy (XAS), which are almost always performed at synchrotron light sources to give suitable sensitivity to biologically realistic concentrations of metal ions. We do not take a comprehensive viewpoint, but rather choose particular applications to illustrate where these methods shine, directly probing the native coordination environment where no other techniques can, providing a complementary picture to that provided by other methods, and acting as a vital sanity check on results derived from other less direct methods.

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