Absorption Characteristics of Azasetron from Rectal and Oral Routes in Rabbits.

Moriyama, Yuhsuke; Arimori, Kazuhiko; Nakano, Masahiro

doi:10.1248/bpb.20.701

Cited by 5 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are few reports on the analysis of azasetron in biological fluids so far [8][9][10]. As far as we know, the simple and efficient column-switching HPLC method for assaying azasetron in plasma has been reported for the first time in the present investigation.…”

Section: Resultsmentioning

confidence: 69%

See 1 more Smart Citation

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

View full text Add to dashboard Cite

For the quantification of azasetron in rat plasma samples, a column-switching HPLC method was developed and validated. Following dilution of plasma samples with mobile phase A (17 mM potassium phosphate buffer (pH 3.0)) and simple protein precipitation by addition of perchloric acid (60%), the mixture was directly injected onto the pre-column. After endogenous plasma substances were eluted to waste, the analyte was transferred to the trap column by switching the system. Then, the analyte was back-flushed to the analytical column for separation with mobile phase B (a 22:78 v/v mixture of acetonitrile and 17 mM potassium phosphate buffer (pH 3.0)) and detected at 250 nm using a photodiode array detector. A linear standard curve was obtained in the concentration range of 10-800 ng/mL with the correlation coefficient (r) of 0.9998. The intra- and inter-day precision and accuracy values for azasetron were in the ranges of 0.3-12.9% and 89.7-101.4%, respectively. The method was valid in terms of specificity, precision, and accuracy. In addition, this efficient analytical method was successfully applied to determine plasma concentrations of azasetron following oral administration of azasetron at a dose of 4.0 mg/kg to rats.

show abstract

Section: Resultsmentioning

confidence: 69%

“…A few HPLC methods have been reported for the determination of azasetron in biological fluids using fluorescence detection [8][9][10]. However, liquid-liquid extraction techniques used in these methods have some limitations such as poor yield and/or laborious and time-consuming extraction processes.…”

Section: Introductionmentioning

confidence: 99%

Simple determination of azasetron in rat plasma by column‐switching high‐performance liquid chromatography

Um¹,

Chae²,

Park³

et al. 2010

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Trichloroacetic acid (10%) in water (w/v) proved to be the best reagent in terms of the peak shape obtained by LC‐MS/MS. It is simpler and more rapid than the liquid–liquid extraction method described in the literature (Moriyama et al ., 1997).…”

Section: Resultsmentioning

confidence: 99%

“…Liquid–liquid extraction by dichloroethane was used as the sample pretreatment. A 0.2 mL serum sample was needed, and 50 µL was injected into the HPLC column for analysis with a lower limit of quantitation (LLOQ) of 50 ng/mL (Moriyama et al ., 1997).…”

Section: Introductionmentioning

confidence: 99%

Determination of azasetron hydrochloride in rabbit plasma by liquid chromatography tandem mass spectrometry and its application

Lin

Zhu

et al. 2010

Biomed. Chromatogr.

View full text Add to dashboard Cite

A sensitive and selective liquid chromatography tandem mass spectrometry method for determination of azasetron hydrochloride in rabbit plasma was developed. After addition of doxapram hydrochloride as internal standard (IS), protein precipitation by 10% trichloroacetic acid was used as sample preparation. Chromatographic separation was achieved on a Zorbax SB-C(18) (2.1 × 50 mm, 3.5 µm) column with acetonitrile-water as mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used to quantification using target fragment ions m/z 349.9 → 223.5 for azasetron hydrochloride and m/z 378.9 → 291.8 for the IS. Calibration plots were linear over the range of 6-1000 ng/mL for azasetron hydrochloride in plasma. The lower limit of quantitation for azasetron hydrochloride was 6 ng/mL. The mean recovery of azasetron hydrochloride from plasma was in the range 85.6-92.7%. The RSDs of intra-day and inter-day precision were both less than 12%. This method is simple and sensitive enough to be used in pharmacokinetic research for determination of azasetron hydrochloride in rabbit plasma.

show abstract