Absorption and metabolism of red orange juice anthocyanins in rats

Felgines, Catherine; Talavéra, Séverine; Texier, Odile; Besson, Catherine; Fogliano, Vincenzo; Lamaison, Jean Louis; Fauci, L. La; Galvano, G.; Rémésy, Christian; Galvano, Fabio

doi:10.1079/bjn20061728

Cited by 55 publications

(45 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analyses were carried out with linear gradient conditions from 100 to 90% A for 10 min and then to 75% A for 30 min. Peak identification and assignment were based on the comparison of their retention time and spectral data with that of the blackberry anthocyanin extract, standards or our previous data obtained by HPLC-MS/MS analysis [18,27]. Anthocyanin quantification was expressed as cyanidin 3-glucoside equivalents.…”

Section: Hplc Analysismentioning

confidence: 99%

Tissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet

Felgines¹,

Texier

Garcin

et al. 2009

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

Anthocyanins are natural dietary pigments that could be involved in various health effects. The aim of this study was to investigate the distribution of anthocyanins to various organs (bladder, prostate, testes, heart and adipose tissue) in rats fed with a blackberry anthocyanin-enriched diet for 12 days. Identification and quantification of anthocyanins were carried out by HPLC-DAD. The urinary excretion of total anthocyanins (native anthocyanins and their metabolites) was low (0.20 +/- 0.03%, n = 8). Proportions of anthocyanin derivatives (methylated anthocyanins and glucurono-conjugated derivatives) differed according to the organ considered. The bladder contained the highest levels of anthocyanins followed by the prostate. Prostate, testes and heart contained native cyanidin 3-glucoside and a small proportion of cyanidin monoglucuronide. Cyanidin 3-glucoside and methylated derivatives were present in adipose tissue. Thus, anthocyanin feeding in rats resulted in a wide distribution of anthocyanin derivatives to several organs. Identification of target tissues of anthocyanins may then help to understand the mechanisms of action of anthocyanins in vivo.

show abstract

Section: Hplc Analysismentioning

confidence: 99%

Tissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet

Felgines¹,

Texier

Garcin

et al. 2009

Molecular Nutrition Food Res

Self Cite

View full text Add to dashboard Cite

show abstract

“…0.1% of the quantities ingested, and sometimes much less, have been detected in plasma and urine [14,15] implying a low level of absorption compared to other flavonoids [13]. However, in situ perfusion studies with rat small intestine indicate that absorption may be of the order of 10 -20%, depending upon the aglycone moiety [18,19].…”

Section: Introductionmentioning

confidence: 95%

The bioavailability of raspberry anthocyanins and ellagitannins in rats

Borges

Roowi

Rouanet

et al. 2007

Molecular Nutrition Food Res

107

View full text Add to dashboard Cite

The fate of anthocyanins and ellagitannins in rats was monitored following ingestion of raspberry juice. After 1 h low nM concentrations of unmetabolised anthocyanins were present in plasma but these declined by 2 h and after 4 h they were no longer detectable. For the first 2 h there was an almost full recovery of anthocyanins as they passed from the stomach through the duodenum/jejunum and into the ileum. After 3 h less than 50% were recovered, and the levels declined rapidly thereafter. Excretion of raspberry anthocyanins in urine over a 24 h period was equivalent to 1.2% of the amount ingested. Trace quantities of anthocyanins were detected in the caecum, colon and faeces and they were absent in extracts of liver, kidneys and brain. Urine also contained a number of phenolic acids but most were present in quantities well in excess of the 918 nmol of anthocyanins present in the ingested juice. These findings indicate that raspberry anthocyanins per se are poorly absorbed, probably prior to reaching the ileum, and that substantial amounts pass from the small to the large intestine where they are degraded by colonic bacteria. Ellagitannins disappeared in the stomach without accumulation of ellagic acid.

show abstract

“…1). Absorption of blood orange juice anthocyanins was studied only in rats [25], while no reports are available on humans. Starting from these considerations, we have first developed an accurate LC-MS/MS methodology for the simultaneous quantitation of blood orange juice anthocyanins C3G, D3G and CMG in human plasma and urine.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of an LC‐MS/MS analysis for simultaneous determination of delphinidin‐3‐glucoside, cyanidin‐3‐glucoside and cyanidin‐3‐(6‐malonylglucoside) in human plasma and urine after blood orange juice administration

Giordano¹,

Coletta²,

Rapisarda

et al. 2007

J of Separation Science

View full text Add to dashboard Cite

Blood orange juice has a high content in anthocyanins, especially represented by delphinidin-3-glucoside (D3G), cyanidin-3-glucoside (C3G) and cyanidin-3-(6-malonylglucoside) (CMG). An LC-MS/MS method for the simultaneous determination of D3G and C3G in human plasma and urine was developed and validated. After sample preparation by SPE, chromatographic separation was performed with an RP-C(18) column, using a water/methanol linear gradient. The quantitation of target compounds was determined by multiple reaction monitoring (MRM) mode, using ESI. The method showed good selectivity, sensitivity (LOD = 0.05 and 0.10 ng/mL for C3G in plasma and urine, respectively; LOD = 0.10 ng/mL for D3G in plasma and urine), linearity (0.20-200 ng/mL; r >or= 0.998), intra- and interday precision and accuracy (

show abstract

Absorption and metabolism of red orange juice anthocyanins in rats

Cited by 55 publications

References 43 publications

Tissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet

Tissue distribution of anthocyanins in rats fed a blackberry anthocyanin‐enriched diet

The bioavailability of raspberry anthocyanins and ellagitannins in rats

Development and validation of an LC‐MS/MS analysis for simultaneous determination of delphinidin‐3‐glucoside, cyanidin‐3‐glucoside and cyanidin‐3‐(6‐malonylglucoside) in human plasma and urine after blood orange juice administration

Contact Info

Product

Resources

About