Absorption and Metabolism of cis‐9,trans‐11‐CLA and of Its Oxidation Product 9,11‐Furan Fatty Acid by Caco‐2 Cells

Abstract

Furan fatty acids (furan-FA) can be formed by auto-oxidation of conjugated linoleic acids (CLA) and may therefore be ingested when CLA-containing foodstuff is consumed. Due to the presence of a furan ring structure, furan-FA may have toxic properties, however, these substances are toxicologically not well characterized so far. Here we show that 9,11-furan-FA, the oxidation product of the major CLA isomer cis-9,trans-11-CLA (c9,t11-CLA), is not toxic to human intestinal Caco-2 cells up to a level of 100 μM. Oil-Red-O staining indicated that 9,11-furan-FA as well as c9,t11-CLA and linoleic acid are taken up by the cells and stored in the form of triglycerides in lipid droplets. Chemical analysis of total cellular lipids revealed that 9,11-furan-FA is partially elongated probably by the enzymatic activity of cellular fatty acid elongases whereas c9,t11-CLA is partially converted to other isomers such as c9,c11-CLA or t9,t11-CLA. In the case of 9,11-furan-FA, there is no indication for any modification or activation of the furan ring system. From these results, we conclude that 9,11-furan-FA has no properties of toxicological relevance at least for Caco-2 cells which serve as a model for enterocytes of the human small intestine.

In‐Vitro Toxicological and Proteomic Analysis of Furan Fatty Acids Which are Oxidative Metabolites of Conjugated Linoleic Acids

“…In a previous study it was shown that 9,11-furan-FA was absorbed by Caco-2 cells and incorporated into triglycerides for storage in lipid droplets [42]. Therefore, assuming that furan-FA must have some effect on Caco-2 cells after absorption, a non-targeted proteomic approach was chosen to examine the furan-FA-dependent effects on the molecular level.…”

Konjuge Li̇nolei̇k Asi̇tleri̇n Önemi̇ Ve Bazi Probi̇yoti̇k Suşlar Tarafindan Üreti̇mi̇

“…‹nsan ve hayvanlar üzerinde yap›lan çal›flmalarda her iki izomerin özellikle farelerde gö¤üs, mide ve kolon kanseri hücrelerinin yay›lmas›na karfl› olumlu etki gösterdi¤i, bunun yan› s›ra organizman›n ya¤ modülasyonu ve ateroskleroz lezyonlar›n›n azalt›lmas› gibi di¤er sa¤l›k etkileriyle de iliflkili oldu¤u tespit edilmifltir (7-14). (13,20). Di¤er önemli bir izomer olan t10,c12 oktadekadienoik asit ise, bitkisel ya¤lar ile florteningler ve margarinler gibi k›smen hidrojene edilmifl ya¤larda bulunmaktad›r.…”