Absorption and metabolism of glycosidic sweeteners of stevia mixture and their aglycone, steviol, in rats and humans

Koyama, Eriko; Sakai, Norifumi; Ohori, Yuji; Kitazawa, Ken; Izawa, Osamu; Kakegawa, Kunio; Fujino, Akiharu; Ui, Michio

doi:10.1016/s0278-6915(03)00039-5

Cited by 112 publications

(73 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Therefore, it is a popular sugar substitute in a variety of foods and often used as a food supplement in Japan, China, South Korea, and Taiwan. Stevioside can be degraded to steviol, an aglycone, by the intestinal microflora from various animal species, including man (Hutapea et al, 1997;Koyama et al, 2003b). Stevioside is a large neutral molecule with both polar and hydrophobic regions, whereas steviol contains a hydrophobic ring and one negative charge on the carboxylic group.…”

mentioning

confidence: 99%

“…They have also been proposed to have a potential role as antihyperglycemic agents by stimulating insulin secretion from pancreatic beta cells (Jeppesen et al, 2000(Jeppesen et al, , 2002(Jeppesen et al, , 2003. Stevioside was not effectively absorbed from everted sacs of rat intestine, whereas steviol was rapidly absorbed (Koyama et al, 2003b). Similarly, stevioside showed very limited absorption by the Caco-2 monolayers and steviol was much better absorbed .…”

mentioning

confidence: 99%

See 1 more Smart Citation

Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)

Srimaroeng¹,

Chatsudthipong²,

Aslamkhan³

et al. 2005

J Pharmacol Exp Ther

View full text Add to dashboard Cite

The natural sweetening agent stevioside and its aglycone metabolite, steviol, have been shown to inhibit transepithelial transport of para-aminohippurate (PAH) in isolated rabbit renal proximal tubules by interfering with basolateral entry. The aim of the present study was to determine which of the cloned basolateral organic anion transporters were involved in the renal transport of stevioside and steviol. This question was addressed in Xenopus laevis oocytes expressing human organic anion transporter 1 (hOAT1), 3 (hOAT3), and winter flounder OAT (fOat1). The parent compound, stevioside, had no inhibitory effect on either PAH (hOAT1) or ES (estrone sulfate; hOAT3) uptake. In contrast, steviol showed significant, dosedependent inhibition of PAH and ES uptake in hOAT1-or hOAT3-expressing oocytes, respectively. The IC 50 of steviol for hOAT1-mediated PAH transport was 11.1 M compared with 62.6 M for hOAT3-mediated ES uptake. The Michaelis-Menten inhibition constants (K i ) for steviol transport mediated by hOAT1 and hOAT3 were 2.0 Ϯ 0.3 and 5.4 Ϯ 2.0 M, respectively. Trans-stimulation of PAH efflux by steviol was assessed to determine whether steviol itself was transported by hOAT1 or hOAT3. A low concentration of 1 M steviol increased the efflux of [3 H]PAH (trans-stimulated) via both hOAT1 and hOAT3. In addition, it was shown by electrophysiology that steviol entry induced inward current in fOat1-expressing oocytes. In conclusion, stevioside had no interaction with either hOAT1 or hOAT3, whereas hOAT1, hOAT3, and fOat1 were all shown to be capable of steviol transport and thus, can play a role in its renal transport and excretion.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)

Srimaroeng¹,

Chatsudthipong²,

Aslamkhan³

et al. 2005

J Pharmacol Exp Ther

View full text Add to dashboard Cite

show abstract

“…Other studies indicate that none of the digestive enzymes in the gastrointestinal tract of different animals and the man are able to degrading stevioside in steviol 84,85 . It is established that eventually is excreted by renal and biliary system 86 .…”

Section: Metabolism and Toxicitymentioning

confidence: 99%

Anticariogenic properties and effects on periodontal structures of Stevia rebaudiana Bertoni. Narrative review.

Contreras¹

2013

J Oral Res

View full text Add to dashboard Cite

“…El esteviósido puede ser degradado a esteviol, por la flora intestinal de diversas especies animales, incluido el hombre (34); el esteviósido es una molécula neutra con ambas regiones polares e hidrofóbicas, mientras que el esteviol contiene un anillo hidrofóbico y una carga negativa en el grupo carboxíli-co. En las ratas, el esteviósido no se absorbe con eficacia, en cambio el esteviol se absorbe rápidamente (34). De la misma forma, el esteviósido muestra una absorción muy limitada por las monocapas de las células Caco-2, en tanto que el esteviol se absorbe mucho mejor (35).…”

Section: Absorción Y Excreción De Esteviaunclassified

Estevia (stevia rebaudiana), edulcorante natural y no calórico

N²,

et al. 2012

Rev. chil. nutr.

View full text Add to dashboard Cite

Absorption and metabolism of glycosidic sweeteners of stevia mixture and their aglycone, steviol, in rats and humans

Cited by 112 publications

References 20 publications

Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)

Transport of the Natural Sweetener Stevioside and Its Aglycone Steviol by Human Organic Anion Transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8)

Anticariogenic properties and effects on periodontal structures of Stevia rebaudiana Bertoni. Narrative review.

Estevia (stevia rebaudiana), edulcorante natural y no calórico

Contact Info

Product

Resources

About