Absorption and fluorescence spectroscopic investigation of indocyanine green

Philip, Reji; Penzkofer, Alfons; Bäumler, Wolfgang; Szeimies, Rolf‐Markus; Abels, Christoph

doi:10.1016/1010-6030(95)04292-x

Cited by 238 publications

(255 citation statements)

References 40 publications

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“…ICG absorbs light primarily in the range of 600 and 900 nm and emits fluorescence light from 750 to 950 nm. It has a molar extinction coefficient of 2 Â 10 4 $ 1 Â 10 5 cm À1 =M, depending on the excitation wavelength, 27 and a quantum yield of $0.027, 28 when the concentration is less than 1 mM in water in its absorption spectrum range. To comparatively evaluate the performance of FMT and PAT, the same ICG containing phantoms were used for both modalities and the reconstructed FMT and PAT images were compared in terms of the image pattern=morphology, spatial resolution, and sensitivity.…”

Section: Experiments and Resultsmentioning

confidence: 99%

Photoacoustic tomography and fluorescence molecular tomography: A comparative study based on indocyanine green

et al. 2012

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Purpose: Both photoacoustic tomography (PAT) and fluorescence molecular tomography (FMT) can be used for molecular imaging when contrast agents are administrated. The goal of this work is to comparatively evaluate the performance of reflection-mode PAT and FMT in common phantom when indocyanine green (ICG) was used as a contrast agent. Methods: Reflection-mode PAT and FMT systems were developed. Target embedded in a background phantom with different ICG concentration, size, and depth location was examined. Comparisons were made in terms of target morphology, spatial resolution, and sensitivity between the two modalities. Results: Phantom results showed that PAT and FMT gave different image morphology. PAT offered higher spatial resolution, while FMT provided higher sensitivity. Thus, improved target detection could be achieved by correlating the complementary information obtained from the two modalities. Conclusions: The combination of high resolution PAT and high sensitivity FMT will provide a more complete range of pathology spectra for more reliable target detection, suggesting a potentially better diagnostic tool when this combination coupled with the administration of ICG as contrast agent is applied to clinical problems in the future.

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Section: Experiments and Resultsmentioning

confidence: 99%

Photoacoustic tomography and fluorescence molecular tomography: A comparative study based on indocyanine green

et al. 2012

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“…After intravenous injection of ICG solution, the dye is retained for 3-4 minutes in the capillaries and then rapidly cleared from the bloodstream by the liver (less than 4% remaining in the plasma 20 min after administration of the initial bolus) [24]. Fluorescence emission of ICG is concentration and solvent dependent [25]. In aqueous solution, ICG in the chosen concentration has very low fluorescence because of the tendency of ICG molecules to aggregate.…”

Section: Discussionmentioning

confidence: 99%

In vivo visualization and ex vivo quantification of experimental myocardial infarction by indocyanine green fluorescence imaging

Sonin¹,

Papayan²,

Почкаева

et al. 2016

Biomed. Opt. Express

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Abstract:The fluorophore indocyanine green accumulates in areas of ischemia-reperfusion injury due to an increase in vascular permeability and extravasation of the dye. The aim of the study was to validate an indocyanine green-based technique of in vivo visualization of myocardial infarction. A further aim was to quantify infarct size ex vivo and compare this technique with the standard triphenyltetrazolium chloride staining. Wistar rats were subjected to regional myocardial ischemia (30 minutes) followed by reperfusion (n = 7). Indocyanine green (0.25 mg/mL in 1 mL of normal saline) was infused intravenously for 10 minutes starting from the 25th minute of ischemia. Video registration in the near-infrared fluorescence was performed. Epicardial fluorescence of indocyanine green corresponded to the injured area after 30 minutes of reperfusion. Infarct size was similar when determined ex vivo using traditional triphenyltetrazolium chloride assay and indocyanine green fluorescent labeling. Intravital visualization of irreversible injury can be done directly by fluorescence on the surface of the heart. This technique may also be an alternative for ex vivo measurements of infarct size.

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“…Stock solutions (~1 mM) of indocyanine green (ICG) and LS172 were prepared in DMSO and serial dilutions were made to obtain an absorbance level of ~0.02 (720 nm). The quantum yield of LS172 (Φ LS172 ) relative to ICG was determined by the equation Φ LS172 = Φ ICG (slope LS172 /slope ICG ), where the slope is determined from a linear regression of integrated fluorescence intensity (735 to 830 nm) versus absorbance (720 nm) of serially diluted LS172 in DMSO (23,24).…”

Section: Spectral Properties Of Ls172mentioning

confidence: 99%

Agonist−Antagonist Dilemma in Molecular Imaging: Evaluation of a Monomolecular Multimodal Imaging Agent for the Somatostatin Receptor

Edwards

Akers

et al. 2007

Bioconjugate Chem.

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The combination of different imaging modalities, each providing information according to its strengths, can be a powerful method for diagnosing diseases. We have synthesized a monomolecular multimodal imaging agent (MOMIA), LS172, containing a subtype-2 somatostatin receptor (SSTr2)-avid peptide (Y3-octreotate or Y3-TATE), a radiometal chelating group (DOTA) and a near-infrared (NIR) fluorescent dye (cypate). In addition to optical methods, radiolabeling LS172 with 64 Cu and 177 Lu provides a strategy for in vitro evaluation or in vivo multimodal imaging by positron emission tomography (PET) and single photon emission computed tomography (SPECT), respectively. Determination of the binding affinity of LS172, nat Cu-and nat Lu-LS172 in SSTr2-transfected A427 cells (A427-7) showed that they all displayed high binding affinity toward SSTr2 with K i values of 0.234 nM, 11.5 nM, and 2.15 nM respectively. In contrast to cypate-labeled Y3-TATE (cytate), fluorescence microscopy showed that LS172 and nat Cu-LS172 accumulate modestly in A427-7 cells by SSTr2-mediated endocytosis, in spite of their relatively high binding affinity. In vivo, the biodistribution of the SSTr2 receptor specific 64 Cu-and 177 Lu-LS172 in AR42J tumor bearing rats exhibited low (≤1% ID/g) accumulation in tumor tissue. Clearance from circulation was predominantly hepatobiliary (>90% ID/liver). Both optical and radionuclear biodistribution studies showed a similar in vivo distribution profile. Surprisingly, the strong binding of LS172 to SSTr2 did not translate into high SSTr2-mediated endocytosis in cells or uptake in tumor in vivo. Considering that LS172 is a putative antagonist, the poor accumulation of the labeled MOMIAs in SSTr2 positive tumor tissue supports the paradigm that agonists with their concomitant internalization favors appreciable target tissue accumulation of receptor-specific ligands.

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Absorption and fluorescence spectroscopic investigation of indocyanine green

Cited by 238 publications

References 40 publications

Photoacoustic tomography and fluorescence molecular tomography: A comparative study based on indocyanine green

Photoacoustic tomography and fluorescence molecular tomography: A comparative study based on indocyanine green

In vivo visualization and ex vivo quantification of experimental myocardial infarction by indocyanine green fluorescence imaging

Agonist−Antagonist Dilemma in Molecular Imaging: Evaluation of a Monomolecular Multimodal Imaging Agent for the Somatostatin Receptor

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