Absolute Population Estimates using Marking Techniques

Southwood, T. R. E.

doi:10.1007/978-94-015-7291-0_3

Cited by 4 publications

References 194 publications

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Serological marking of Pnigalio agraules (Hymenoptera: Eulophidae) for field dispersal studies

et al. 2008

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The parasitoid wasp Pnigalio agraules (Wlk.) is a key natural enemy of the horsechestnut leafminer Cameraria ohridella Deschka and DimiT (Lepidoptera: Gracillariidae). As a basis for mark-release-recapture studies, aimed at investigating the dispersal of this parasitoid in the Weld, adults of P. agraules were marked using a vertebrate-speciWc immunoglobulin (IgG). The marker was later detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA). The IgG was either applied externally by spraying or internally by feeding an IgG-enriched diet. DiVerent concentrations of the marker were used and the inXuence of abiotic (climatic conditions, time elapsed between marking and marker examination) and biotic factors (sex and age of the parasitoids) on the detection of the immunomarker was tested. External marking by spraying led to more homogeneous labelling than feeding the marker. Parasitoids labelled with 0.25 mg rabbit IgG per ten individuals contained enough immunomarker to be easily distinguished from unmarked ones. Neither the climatic conditions nor the sex or age of the insects had an inXuence on the detection of the marker. The IgG remained well detectable during the entire lifespan of the parasitoids, which was not negatively aVected by the marking procedure. Serological marking can be used to investigate the dispersal behaviour of beneWcial insects within markrelease-recapture studies.

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Serological marking of Pnigalio agraules (Hymenoptera: Eulophidae) for field dispersal studies

et al. 2008

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The Influence of Marking Methods on Mobility, Survivorship, and Field Recovery of Halyomorpha halys (Hemiptera: Pentatomidae) Adults and Nymphs

Kirkpatrick

Rice

Ibrahim

et al. 2020

Environmental Entomology

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Halyomorpha halys (Stål), the brown marmorated stink bug, is an invasive and highly polyphagous insect that has caused serious economic injury to specialty and row crops in the United States and Europe. Here, we evaluated the effects of marking adult and nymphal H. halys with four different colors of fluorescent powder (Blaze Orange, Corona Pink, Horizon Blue, and Signal Green) on mobility and survivorship in laboratory bioassays. Adults and nymphs were marked using liquified fluorescent powder solutions and allowed to dry prior to bioassay. The presence of the marking solution had no significant effects on adult or nymphal mobility, adult survivorship, nymphal development, or adult flight capacity. We also evaluated the persistence of neon marker applied to the pronotum of H. halys adults and found this technique remained detectable for 2 wk under field conditions. Although both marking techniques are inexpensive, persist for ≥1 wk, and do not affect mortality, the neon marker method is more time-consuming, taking ~12 times longer to mark 50 adult H. halys compared with the liquified fluorescent powders. Thus, we would recommend using fluorescent powders for large-scale mark-release-recapture studies.

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Photoperiod-Specific Within-Plant Distribution of the Green Stink Bug (Hemiptera: Pentatomidae) on Cotton

Glover

Sword

Brewer

2019

Environmental Entomology

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Sampling methods for detecting stink bugs are intensive, time-consuming, and yield variable results. In a 2-yr mark-release-observe experiment, over 500 adult green stink bugs, Chinavia hilaris (Say) (Hemiptera: Pentatomidae), were used to test for variation in nocturnal and diurnal insect distribution patterns on cotton. Field-collected stink bugs were marked or unmarked with nontoxic fluorescent sharpie markers, released, and monitored in cotton fields at peak bloom. Stink bugs were monitored visually during day and night, aided by a handheld blacklight for nighttime observations. Within-cotton distribution insect observations were categorized by plant section (i.e., bottom, middle, and top branches), by fruiting positions and leaf surface, and by concealed or exposed orientation on floral bracts and leaf surfaces. Green stink bugs were primarily distributed on the middle and top branches irrespective of photoperiod, and on bolls in first position from the main stem. Differences in stink bugs observed concealed or exposed on fruiting structures were detected. During daytime, stink bugs were primarily observed inside the bract of bolls, and when detected on leaves concealed on the lower surface. In contrast, stink bugs were primarily outside the bract of bolls at night, and when detected on leaves were exposed on an upper surface. These results support focus on assessing internal boll injury for evaluating stink bug injury to avoid the challenges in stink bug detection observed here, and point to additional study to refine stink bug density estimation when needed.

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Absolute Population Estimates using Marking Techniques

Cited by 4 publications

References 194 publications

Serological marking of Pnigalio agraules (Hymenoptera: Eulophidae) for field dispersal studies

Serological marking of Pnigalio agraules (Hymenoptera: Eulophidae) for field dispersal studies

The Influence of Marking Methods on Mobility, Survivorship, and Field Recovery of Halyomorpha halys (Hemiptera: Pentatomidae) Adults and Nymphs

Photoperiod-Specific Within-Plant Distribution of the Green Stink Bug (Hemiptera: Pentatomidae) on Cotton

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