Absence of DOMR, a new antigen in the Dombrock blood group system that weakens expression of Do^b, Gy^a, Hy, Jo^a, and DOYA antigens

Abstract

The antibody in the patient's plasma recognizes the high-prevalence antigen absent from her RBCs. The Ala144Glu change caused an absence of a high-prevalence Do antigen that we have named DOMR [provisional ISBT number 014007 (DO7)]. The absence of DOMR is associated with weakening of Do(b) , Gy(a) , Hy, Jo(a) , and DOYA antigens.

Synonymous nucleotide polymorphisms influence Dombrock blood group protein expression in K562 cells

“…In our K562 model, protein expression by the DO*DOMR allele was lower than that of the DO*B transductants. This finding is in agreement with weak reactivity observed by haemagglutination testing using MIMA-52 and MIMA-53 on patient RBCs typed as Do (a-b+ w ), Gy(a+ w ), Hy+ w , Jo(a+ w ) and DOYA+ w (Costa et al, 2010).…”

“…Do a (DO1, International Society of Blood Transfusion [ISBT] reference 014001) and Do b (DO2, ISBT 014002), and a total of six high-incidence antigens, i.e. Gy a , Hy, and Jo a , plus the recently described DOYA, DOMR and DOLG antigens (Costa et al, 2010;Mayer et al, 2010;Karamatic Crew et al, 2011). These antigens are carried on the Dombrock glycoprotein (40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54)(55)(56)(57)(58) that is attached to the red blood cell (RBC) membrane via glycosylphosphatidylinositol (GPI) linkage (Telen et al, 1990;Spring & Reid, 1991;Spring et al, 1994).…”

Dombrock Blood Group System

“…DOMR − results from homozygosity for two nucleotide changes within the same codon, 431C> A and 432C > A, encoding Ala144Glu [46] . Her red cells were Do(a− b + ), weak for Hy and Jo a , and non -reactive with alloanti -Gy a , but reactive with monoclonal antibodies to the Dombrock protein.…”

“…Like anti -Do a and -Do b , the other Dombrock system antibodies have never been implicated in HDFN, despite numerous opportunities, though the baby of the woman with anti -DOMR had DAT -positive red cells and was jaundiced, but only required phototherapy [46] . One anti -Hy has been responsible for an HTR in a man who received two units of Hy + blood [35] .…”

International Society of Blood Transfusion Working Party on red cell immunogenetics and blood group terminology: Berlin report

“…DO7 (DOMR) is defined by an antibody to a high incidence antigen produced in a patient, homozygous for two adjacent nucleotide changes 431C>A, 432C>A that encode a change of Ala144Glu [13]. These changes are present with a DO*B-WL allele ( DO*793G, DO*323G, DO*350C, DO*547T, DO*898G ).…”