Absence of a microbiome in the midgut trunk of six representative Crustacea

Martin, Gary G.; Natha, Zain; Henderson, Nicola; Bang, Sabi; Hendry, Hope; Loera, Yeraldi

doi:10.1093/jcbiol/ruz087

Cited by 17 publications

(21 citation statements)

References 77 publications

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“…According to our observations, Deferribacteres cells are in direct contact with their host. This contradicts previous reports on shrimps and crustaceans more generally (Martin et al, 2020; Vogt, 2021). In Artemia salina , Daphnia magna , Gammarus sp., Homarus americanus , Tigriopus californicus and Sicyonia ingentis , microorganisms appear in the midgut tube and hindgut of crustaceans, located in the alimentary bolus within the endoperitrophic space (i.e.…”

Section: Discussioncontrasting

confidence: 99%

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

Guéganton

Rouxel

Durand

et al. 2022

Preprint

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The shrimps Rimicaris exoculata and Rimicaris chacei are visually dominant fauna co-occurring at deep-sea hydrothermal sites of the Mid-Atlantic Ridge (MAR). Their co-existence was related to contrasted life-history traits, among which differences in their diet and reliance on chemoautotrophic symbionts at adult stage. Both shrimps are colonized by diversified chemosynthetic symbiotic microbial communities in their cephalothoracic cavity. Symbiotic association with bacteria was also evidenced in their digestive system, and the major lineages were identified through sequencing (Mycoplasmatales lineages mainly in the foregut and Deferribacteres lineages mainly in the midgut) but their clear distribution within each host species was not assessed. For the first time, we used Fluorescence in situ Hybridization (FISH) to visualize these lineages. Then, we described their association with digestive structures of both Rimicaris species. The aim was to identify possible differences between host species that could be related to their different life-history traits. For this purpose, we first developed specific FISH probes targeting Deferribacteres and Mycoplasmatales lineages identified in the digestive system of these shrimps. After signal specificity validation for each the new probe, we showed a partitioning of the bacterial lineages according to the digestive organ. Despite morphological differences between the foregut of R. exoculata and R. chacei that could be related to the adult diet, our FISH results showed overall similar distribution of digestive symbionts for the two host species. However, a more comprehensive study is needed with specimens at different life or molt stages to bring potentially host specific patterns out. Such comparative approach using FISH is now warranted thanks to our newly designed probes. These will be valuable tools to track symbiont lineages in the environment, allowing a better understanding of their relationship with their host along its life cycle, including acquisition mechanisms.

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Section: Discussioncontrasting

confidence: 99%

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

Guéganton

Rouxel

Durand

et al. 2022

Preprint

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“…Most arthropods construct a physical barrier composed of chitin in their midgut called a peritrophic matrix (PM) 4 , 5 . This suggests that the intestinal microbiota that colonize the gut epithelia is a derived feature of mammal, which lacked in other organisms 6 , including crustacean 7 .…”

Section: Introductionmentioning

confidence: 99%

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Imaizumi

Tinwongger

Kondo

et al. 2021

Sci Rep

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In mammals, the intestine harbors numerous bacteria that play an important role in health. Intestinal microbiota have also been thought to be an important factor in the health of shrimp. However, the barrier systems of the digestive tracts of shrimp seem to be different from those of mammals. In this study, we analyzed the bacterial composition in the stomach and midgut of two species of shrimp during administration of a probiotic, Bacillus amyloliquefaciens strain TOA5001 by analysis of 16S rRNA genes with Illumina sequencing technology. Whiteleg shrimp Litopenaeus vannamei were observed under laboratory conditions and kuruma shrimp Marsupenaeus japonicus were observed in an aquaculture farm. The diversities of bacteria in the stomachs of both shrimps were significantly higher than those in the midgut. Also, the microbiota changed during probiotic feeding. Feeding whiteleg shrimp the probiotic after being challenged with an acute hepatopancreatic necrosis disease (AHPND)-causing strain of Vibrio parahaemolyticus increased their survival compared to the control group, which suggested that the probiotic prevented AHPND. These results appear to show that a probiotic can affect the microbiota throughout digestive tract of penaeid shrimps and that probiotic can have a role in preventing disease.

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“…However, the microbiome present on the feed given to lobsters was not assessed. This is important because Martin et al (2020) showed that the lumen of the midgut of American lobsters, as well as 5 other crustaceans from disparate taxa, have no microbiome per se. The microbiome is solely present within the food bolus which is entirely encased by the peri trophic membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Although there has been no work on the gut microbiome of the American lobster, a recent study has examined how the formation of gut microbiome changes in the European lobster H. gammarus (Holt et al 2020). In addition, using ultrastructure and specialized staining, Martin et al (2020) found that American lobsters and several other crustaceans do not have an active microbiome in the midgut region because the food bolus is encased in a peritrophic membrane that excludes bacteria from entering the lumen of the midgut.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of the hemolymph microbiome between live and recently dead American lobsters Homarus americanus

et al. 2021

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Lobsters and other crustaceans do not have sterile hemolymph. Despite this, little is known about the microbiome in the hemolymph of the lobster Homarus americanus. The purpose of this study was to characterize the hemolymph microbiome in lobsters. The lobsters were part of a larger study on the effect of temperature on epizootic shell disease, and several died during the course of the study, providing an opportunity to examine differences in the microbiomes between live and recently dead (1-24 h) animals. The hemolymph microbiomes of live lobsters was different from those in dead animals and both were different from the tank microbiome in which the animals had been held. The microbiomes of live lobsters were more diverse and had a different suite of bacteria than those from dead animals. The dominant taxa in live lobsters belonged to Flavobacteriaceae and Rhodobacteraceae, whereas Vibrionaceae and Enterobacteriaceae were predominant in the dead lobsters. Although aquarium microbiomes overlapped with the hemolymph microbiomes, there was less overlap and lower abundance of taxa in comparison with hemolymph from live lobsters. Previous studies reporting bacteria in the digestive tract of lobsters suggested that Vibrionaceae and Enterobacteriaceae had invaded the hemolymph via the gut. Our study suggests that hemolymph bacteria abundant in live lobsters do not originate from the tank milieu and comprise a rich, natural, or native background of bacterial constituents.

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Absence of a microbiome in the midgut trunk of six representative Crustacea

Cited by 17 publications

References 77 publications

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

Anatomy and Symbiosis of the digestive system of the vent shrimps Rimicaris exoculata and Rimicaris chacei revealed through imaging approaches

Analysis of microbiota in the stomach and midgut of two penaeid shrimps during probiotic feeding

Comparative study of the hemolymph microbiome between live and recently dead American lobsters Homarus americanus

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