Abscisic acid, polyamines and phenolic acids in sessile oak somatic embryos in relation to their conversion potential

Cvikrová, Milena; Malá, Jana; Eder, J.; Hrubcová, Marie; Vágner, Martin

doi:10.1016/s0981-9428(97)86882-9

Cited by 35 publications

(16 citation statements)

References 30 publications

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“…Genes encoding enzymes in the phenylpropanoid pathway are up-regulated during barley (Hordeum vulgare) microspore embryogenesis upon stress treatment of the anthers (Jacquard et al, 2009), and alterations in this pathway have been correlated with the developmental potential of sessile oak (Quercus spp.) somatic embryos (Cvikrova et al, 1998(Cvikrova et al, , 2003. "Flavonoid biosynthesis" was one of the pathways overrepresented in superembryogenic Medicago truncatula (line 2HA) explants compared with its comparatively nonembryogenic progenitor Jemalong.…”

Section: Functional Classification Of Gmagl15-responsive Genesmentioning

confidence: 99%

Alterations in the Transcriptome of Soybean in Response to Enhanced Somatic Embryogenesis Promoted by Orthologs of AGAMOUS-Like15 and AGAMOUS-Like18

Zheng

Perry

2014

Plant Physiol.

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Section: Functional Classification Of Gmagl15-responsive Genesmentioning

confidence: 99%

Alterations in the Transcriptome of Soybean in Response to Enhanced Somatic Embryogenesis Promoted by Orthologs of AGAMOUS-Like15 and AGAMOUS-Like18

Zheng

Perry

2014

Plant Physiol.

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“…This has been observed in several SE systems, where either no shoot meristem was formed such as in Q. rubra (Rancillac et al, 1996) or malformed such as in Q. robur (Zegzouti and Favre, 1997). Several reports indicate that there is now more attention on the somatic embryo quality, including selection for a certain size, other morphological characteristics related to well-formed embryos such as somatic embryos with ivory, light green cotyledons versus deep green cotyledons (Cvikrova et al, 1998;Mala Â et al, 1999), or opaque, white-yellowish color (Bueno et al, 1992;FernandezGuijarro et al, 1995). The major reserve product in zygotic embryos of Q. robur is starch.…”

Section: Maturation and Germinationmentioning

confidence: 99%

Somatic embryogenesis in oak (Quercus spp.)

Wilhelm

2000

In Vitro Cell.Dev.Biol.-Plant

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The present review summarizes the factors involved in controlling the process of oak somatic embryogenesis as a method for vegetative plant propagation and includes also data on artificial seed production, cryopreservation and transformation. One major limitation, the inability to initiate embryogenic cultures from mature trees, has been recently overcome. Leaves from selected cork oak trees with an age of 50 yr and more have been used to initiate somatic embryogenesis (SE) with a frequency of up to 20%. These findings offer encouraging prospects for cloning proven superior plant material and to integrate this propagation system into tree improvement programs. Once the process of SE has been initiated, the multiplication cycle proceeds via secondary embryogenesis, which can be maintained indefinitely. Problems are reported by the formation of anomalous embryos. The mutability of somatic embryogenic cell lines of various oak species has been monitored by flow cytometry and molecular markers. No somaclonal variation was detected applying random amplified polymorphic DNA (RAPD) or amplified fragment length polymorphism (AFLP) markers, whereas DNAcontent measurements via flow cytometry revealed tetraploidy in some cell lines after several years of continuous subculture. Maturation and low germination frequencies are the main bottlenecks for a broader use of this technique. Recently attention has been on embryo quality and parameters for conversion capacity such as high endogenous cytokinin level and low abscisic acid (ABA) level. Although oak is probably the species that is the most well-developed system for a broadleaved forest tree, data on growth performances of somatic embryo-derived plants are rare.

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“…This was later found to be associated with alterations of phenolic compounds bound to cell walls, particularly with an increased content of ferulic acid in regenerable tissues (Lozovaya et al 1996), which could lead to restriction of extensibility of the cell wall (Kamisaka et al 1990) that controls cell expansion and results in formation of diVerentiated clusters of small size cells (Emons and Kieft 1990). The content of cell wall bound ferulic acid in oak somatic embryos that converted into plantlets was three times that of nonconverting embryos with no diVerences found in total content of wall bound phenolic acids (Cvikrova et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Biochemical features of maize tissues with different capacities to regenerate plants

Lozovaya

Ulanov

Lygin

et al. 2006

Planta

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Metabolic profiling using GC-MS and LC-MS analyses of soluble metabolites and cell wall bound phenolic compounds from maize calluses of different morphogenic competence revealed a number of biochemical characteristics that distinguish tissues with high plant regeneration ability from tissues that cannot efficiently regenerate plants in vitro. Maize cultures of different ages from H99 (compact type I callus) and HiII (friable type II callus) were divided into two different samples: regenerable (R) and non-regenerable (NR) based on known morphologies. Tissues from both genotypes with high morphogenic potential had higher asparagine and aspartate and indole-3-butenol concentrations, decreased sugar and DIMBOA (2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one) concentrations, low levels of 4-aminobutyric acid (GABA) and chlorogenic acid and lower levels of feruloyl- and sinapoyl glucosides compared to NR tissues. The ether bound cell wall phenolics of tissues with high regeneration potential had higher levels of the predominant G (guaiacyl) units and lower levels of H (p-hydroxyphenyl) and S (syringyl) units and higher ferulic acid/coumaric acid and ferulic acid/diferulic acid ratios. The same trends were found with the ester-bound phenolics of HiII, however, there were only small differences between the H99 R and NR tissues. Concentrations of the major sugars, organic acids, amino acids and soluble aromatic compounds tended to increase as the time after culture initiation increased. The results show that there are differences in general metabolism, phenolic secondary compounds and cell wall composition between R and NR cell types.

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Abscisic acid, polyamines and phenolic acids in sessile oak somatic embryos in relation to their conversion potential

Cited by 35 publications

References 30 publications

Alterations in the Transcriptome of Soybean in Response to Enhanced Somatic Embryogenesis Promoted by Orthologs of AGAMOUS-Like15 and AGAMOUS-Like18

Alterations in the Transcriptome of Soybean in Response to Enhanced Somatic Embryogenesis Promoted by Orthologs of AGAMOUS-Like15 and AGAMOUS-Like18

Somatic embryogenesis in oak (Quercus spp.)

Biochemical features of maize tissues with different capacities to regenerate plants

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