Abscisic acid control of seed dormancy expression in Nicotiana plumbaginifolia and Arabidopsis thaliana.

Jullien, Marc; Bouinot, Denise; Ali-Rachedi, Sonia; Sotta, Bruno; Grappin, Philippe; Viémont, Jean-Daniel; Crabbé, J.

doi:10.1079/9780851994475.0195

Cited by 8 publications

(11 citation statements)

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“…This is supported by results obtained by Ullah and co-workers (2002) who show that fluridone pre-treatment leads to glucose insensitivity during germination. It has been found that ABA levels drop rapidly in seeds after fluridone treatment Jullien et al 2000). Thus it remains unclear whether the effect of fluridone pre-treatment on glucose inhibition is due to a reduced ABA content or to the inability to increase ABA levels.…”

Section: Complex Interactions Between Sugar and Hormones During Germimentioning

confidence: 96%

“…Seeds were germinated on media supplemented with fluridone, an inhibitor of ABA biosynthesis. ABA biosynthesis is inhibited at low concentrations (10 lM) of fluridone, which is already effective on imbibed seeds within hours following application Jullien et al 2000). De-novo ABA biosynthesis was shown to be necessary to maintain dormancy in imbibed seeds .…”

Section: Glucose Does Not Affect Aba Biosynthesis or Signalling Via Amentioning

confidence: 99%

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Glucose delays seed germination in Arabidopsis thaliana

Dekkers

Schuurmans

Smeekens

2004

Planta

140

112

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Here we report that glucose delays germination of Arabidopsis thaliana (L.) Heynh. seeds at concentrations below those known to inhibit early seedling development. This inhibition acts on embryo growth and is independent of hexokinase (HXK) function. Hormones and hormone inhibitors were applied to the germination media and several hormone biosynthesis and signalling mutants were tested on glucose media to investigate a possible role of abscisic acid (ABA), gibberellin and ethylene in the glucose-induced germination delay. Results indicate that the germination inhibition by glucose cannot be antagonized by ethylene or gibberellin and is independent of the HXK1/ABA/ABI4 signalling cascade. These findings suggest that there is a separate regulatory pathway independent of ABI2/ ABI4/ABI5. Thus, in a relatively short time frame sugars utilize different signalling cascades to inhibit germination and post-germination growth, underlining the complexity of sugar responses.Keywords Abscisic acid AE Arabidopsis AE Ethylene AE Germination AE Gibberellic acid AE Glucose signallingGibberellic acid AE HXK Hexokinase AE LEC1 Leafy cotyledon1 AE RBCS Ribulose-1,5-bisphosphate carboxylase small subunit AE WT Wild type

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Section: Complex Interactions Between Sugar and Hormones During Germimentioning

confidence: 96%

Section: Glucose Does Not Affect Aba Biosynthesis or Signalling Via Amentioning

confidence: 99%

Glucose delays seed germination in Arabidopsis thaliana

Dekkers

Schuurmans

Smeekens

2004

Planta

140

112

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“…Fluridone and GA treatments induce germination of dormant seeds of N. plumbaginifolia and Arabidopsis Jullien et al, 2000). De novo ABA synthesis and suppression of GA synthesis may be required for maintaining dormancy as well as for thermoinhibition of the after-ripened seeds.…”

Section: Function Of Ga Negative Regulators On Thermoinhibitionmentioning

confidence: 99%

High Temperature-Induced Abscisic Acid Biosynthesis and Its Role in the Inhibition of Gibberellin Action in Arabidopsis Seeds

et al. 2008

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Suppression of seed germination at supraoptimal high temperature (thermoinhibiton) during summer is crucial for Arabidopsis (Arabidopsis thaliana) to establish vegetative and reproductive growth in appropriate seasons. Abscisic acid (ABA) and gibberellins (GAs) are well known to be involved in germination control, but it remains unknown how these hormone actions (metabolism and responsiveness) are altered at high temperature. Here, we show that ABA levels in imbibed seeds are elevated at high temperature and that this increase is correlated with up-regulation of the zeaxanthin epoxidase gene ABA1/ZEP and three 9-cis-epoxycarotenoid dioxygenase genes, NCED2, NCED5, and NCED9. Reverse-genetic studies show that NCED9 plays a major and NCED5 and NCED2 play relatively minor roles in high temperature-induced ABA synthesis and germination inhibition. We also show that bioactive GAs stay at low levels at high temperature, presumably through suppression of GA 20-oxidase genes, GA20ox1, GA20ox2, and GA20ox3, and GA 3-oxidase genes, GA3ox1 and GA3ox2. Thermoinhibition-tolerant germination of loss-of-function mutants of GA negative regulators, SPINDLY (SPY) and RGL2, suggests that repression of GA signaling is required for thermoinibition. Interestingly, ABA-deficient aba2-2 mutant seeds show significant expression of GA synthesis genes and repression of SPY expression even at high temperature. In addition, the thermoinhibition-resistant germination phenotype of aba2-1 seeds is suppressed by a GA biosynthesis inhibitor, paclobutrazol. We conclude that high temperature stimulates ABA synthesis and represses GA synthesis and signaling through the action of ABA in Arabidopsis seeds.

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“…Also, Okamoto et al (2006) showed that seeds of the Arabidopsis cyp707a1 and cyp707a2 mutants of the Columbia (Col) accession, which are altered in ABA catabolism, accumulate the phytohormone at higher levels and exhibit enhanced dormancy compared to the wild type. ABA has also been shown to play an essential role in the expression of dormancy in imbibed seeds of several species, as Arabidopsis (Garciarrubio et al, 1997;Jullien et al, 2000;AliRachedi et al, 2004), sunflower (Helianthus annuus; Garello et al, 2000), sweet scented tobacco (Nicotiana plumbaginifolia; Grappin et al, 2000), barley (Hordeum vulgare; Wang et al, 1995;Jacobsen et al, 2002), and lettuce (Lactuca sativa; Toyomasu et al, 1994;Yoshioka et al, 1998). In agreement with this, the application of physiological concentrations of exogenous ABA to ND seeds is well known to inhibit their germination (Milborrow, 1974;Garciarrubio et al, 1997;Gonai et al, 2004), whereas germination of ABA-insensitive Arabidopsis mutants (abi1 to abi5) remains unaffected by such treatment (Koornneef et al, 1984;Finkelstein, 1994).…”

mentioning

confidence: 99%

Proteomic Analysis of Seed Dormancy in Arabidopsis

et al. 2006

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The mechanisms controlling seed dormancy in Arabidopsis (Arabidopsis thaliana) have been characterized by proteomics using the dormant (D) accession Cvi originating from the Cape Verde Islands. Comparative studies carried out with freshly harvested dormant and after-ripened non-dormant (ND) seeds revealed a specific differential accumulation of 32 proteins. The data suggested that proteins associated with metabolic functions potentially involved in germination can accumulate during after-ripening in the dry state leading to dormancy release. Exogenous application of abscisic acid (ABA) to ND seeds strongly impeded their germination, which physiologically mimicked the behavior of D imbibed seeds. This application resulted in an alteration of the accumulation pattern of 71 proteins. There was a strong down-accumulation of a major part (90%) of these proteins, which were involved mainly in energetic and protein metabolisms. This feature suggested that exogenous ABA triggers proteolytic mechanisms in imbibed seeds. An analysis of de novo protein synthesis by two-dimensional gel electrophoresis in the presence of [ 35 S]-methionine disclosed that exogenous ABA does not impede protein biosynthesis during imbibition. Furthermore, imbibed D seeds proved competent for de novo protein synthesis, demonstrating that impediment of protein translation was not the cause of the observed block of seed germination. However, the two-dimensional protein profiles were markedly different from those obtained with the ND seeds imbibed in ABA. Altogether, the data showed that the mechanisms blocking germination of the ND seeds by ABA application are different from those preventing germination of the D seeds imbibed in basal medium.

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Abscisic acid control of seed dormancy expression in Nicotiana plumbaginifolia and Arabidopsis thaliana.

Cited by 8 publications

References 0 publications

Glucose delays seed germination in Arabidopsis thaliana

Glucose delays seed germination in Arabidopsis thaliana

High Temperature-Induced Abscisic Acid Biosynthesis and Its Role in the Inhibition of Gibberellin Action in Arabidopsis Seeds

Proteomic Analysis of Seed Dormancy in Arabidopsis

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