ABO genotyping by TaqMan assay and allele frequencies in a Japanese population

Maeda, Kazuho; Nakamura, Shigeki; Murakami, Chikako; Irie, Wataru; Watanabe, T; Sasaki, Chizuko; Nakamaru, Naomi; Furukawa, Masashi; Kurihara, Katsuyoshi

doi:10.1016/j.legalmed.2012.08.009

Cited by 18 publications

(17 citation statements)

References 21 publications

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“…Rare genotypes such as cis-AB01/O02 , cis-AB01/O01 , and cis-AB01/A102 were conspicuously absent in the current study with these latter alleles being detected among 13 different genotypes in the Korean population [8]. In addition, the cis-AB01 genotype has been reported in Japanese and Chinese populations [56]. The absence of these genotypes cis-AB01/O02 , cis-AB01/O01 and cis-AB01/ A102 in studies from the Middle East indicates that the Middle Eastern population differs considerably from the Chinese, Korean, and Japanese populations in respect to these rare genotypes.…”

Section: Discussionmentioning

confidence: 51%

“…This variation has been widely observed in many studies [56821222324]. In the majority of populations examined, the A101 and A102 alleles are common, whereas the O02 allele is predominant in the Saudi population.…”

Section: Discussionmentioning

confidence: 71%

“…For example, the A101 , O01 , B101 , A102 , and O02 alleles are common in Asian populations [14]. Alleles such as O04 and A205 are found in the Han Chinese, and allele O06 is found in the Japanese [56]. Rare alleles such as cis-AB01 , cis-AB02 , and cis-AB06 are more frequently encountered in the Japanese, Korean, and Chinese populations [578].…”

Section: Introductionmentioning

confidence: 99%

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Allelic variance amongABOblood group genotypes in a population from the western region of Saudi Arabia

et al. 2016

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BackgroundCharacterization of the ABO blood group at the phenotype and genotype levels is clinically essential for transfusion, forensics, and population studies. This study elucidated ABO phenotypes and genotypes, and performed an evaluation of their distribution in individuals from the western region of Saudi Arabia.MethodsOne-hundred and seven samples underwent standard serological techniques for ABO blood group phenotype analysis. ABO alleles and genotypes were identified using multiplex polymerase chain reaction, and electrophoretic analysis was performed to evaluate the highly polymorphic ABO locus.ResultsA phenotype distribution of 37.4%, 30.8%, 24.3%, and 7.5% was found for blood groups O, A, B, and AB respectively in our study cohort. Genotype analysis identified 10 genotype combinations with the O01/O02 and A102/O02 genotypes being the most frequent with frequencies of 33.6% and 14.95%, respectively. Common genotypes such as A101/A101, A101/A102, A101/B101, B101/B101, and O01/O01 were not detected. Similarly, the rare genotypes, cis-AB01/O02, cis-AB01/O01, and cis-AB01/A102 were not found in our cohort. The most frequently observed allele was O02 (35.98%) followed by the A102 allele (17.76%). Furthermore, our findings are discussed in reference to ABO allele and genotype frequencies found in other ethnic groups.ConclusionThe study has a significant implication on the management of blood bank and transfusion services in Saudi Arabian patients.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 99%

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Allelic variance amongABOblood group genotypes in a population from the western region of Saudi Arabia

et al. 2016

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“…Therefore, it is important to use a reliable detection method with high sensitivity for fetal ABO genotyping. The commonly used techniques for ABO genotyping include single-strand conformation polymorphism (SSCP) [22], amplified product length polymorphism (APLP) [23], PCR-restriction fragment length polymorphism (PCR-RFLP) [24], PCR-SSP [25], TaqMan-based and SYBR-based real-time PCR [11,26]. Among these, real-time PCR is the best option to amplify the short DNA sequences at low template concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Among these, real-time PCR is the best option to amplify the short DNA sequences at low template concentration. Recent reports showed that the detection limit of TaqMan-based real-time PCR for ABO genotyping reached 0.1 ng per reaction, while the detection limit of SYBR real-time PCR was 0.5 ng per reaction [11,26]. Unfortunately, the TaqMan-based real-time PCR with allele-specific probes is infeasible for fetal ABO genotyping in our study due to the large background of maternal O gene, which resulted in the dominant amplification signals of maternal O allele overwhelming the weak signals of fetal A/B allele.…”

Section: Discussionmentioning

confidence: 99%