A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes

Zhou, Shihang; Liu, Ming; An, Wanxin; Liang, Xiaohua; Wang, Yu; Piao, Fengyuan

doi:10.1002/jcla.21751

Cited by 5 publications

(6 citation statements)

References 26 publications

(27 reference statements)

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“…Duffy blood group allele frequencies in the Thai population and other Asian populations were compared. The observed allele frequencies were similar to those found in Chinese populations [ 17 21 ] and were significantly different from those of Japanese, Korean, and Malaysian populations ( Table 2 ) [ 14 22 23 ].…”

Section: Resultssupporting

confidence: 81%

“…This is the first report of FY blood group allele frequencies in the Thai population. The FY * A allele was the most common and its frequency was similar to that observed in Chinese populations [ 17 21 ]; but it was statistically significantly different ( P <0.05) from that in Korean, Japanese, and Malaysian populations [ 14 22 23 ]. A recent study compared the Kidd blood group allele frequencies in two Thai blood donors [ 26 ], and found that Kidd blood group allele frequencies in Thai individuals were statistically different from those of Japanese individuals, but similar to those of Chinese individuals [ 21 ].…”

Section: Discussionsupporting

confidence: 53%

“…Current DNA technology for FY blood group genotyping enables the detection of FY alleles. Various PCR-based methods including allele-specific PCR (AS-PCR), PCR-restriction fragment length polymorphism (PCR-RFLP), PCR with sequence-specific primer (PCR-SSP) as single or multiplex assays, real-time quantitative PCR, high-resolution melting analysis, and DNA microarray hybridization have been used for blood group genotyping [ 11 12 14 15 16 17 18 ]. Although the Duffy blood group phenotypes in Thai blood donors have been studied [ 5 19 ], the FY allele frequencies in this group have not been investigated to date.…”

Section: Introductionmentioning

confidence: 99%

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Duffy Blood Group Genotyping in Thai Blood Donors

et al. 2015

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BackgroundDuffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed.MethodsFive hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included. Only 200 samples were tested with anti-Fya and anti-Fyb using the gel technique. All 500 samples and four samples from a Guinea family with the Fy(a-b-) phenotype were genotyped by using PCR-SSP. Additionally, the probability of obtaining antigen-negative red blood cells (RBCs) for alloimmunized patients was calculated according to the estimated FY allele frequencies.ResultsThe FY phenotyping and genotyping results were in 100% concordance. The allele frequencies of FY*A and FY*B in 500 central Thais were 0.962 (962/1,000) and 0.038 (38/1,000), respectively. Although the Fy(a-b-) phenotype was not observed in this study, FY*BES/FY*BES was identified by PCR-SSP in the Guinea family and was confirmed by DNA sequencing.ConclusionsOur results confirm the high frequency of the FY*A allele in the Thai population, similar to that of Asian populations. At least 500 Thai blood donors are needed to obtain two units of antigen-negative RBCs for the Fy(a-b+) phenotype.

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Section: Resultssupporting

confidence: 81%

Section: Discussionsupporting

confidence: 53%

Section: Introductionmentioning

confidence: 99%

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Duffy Blood Group Genotyping in Thai Blood Donors

et al. 2015

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“…This assay can be subject to multiplexing, allowing for the simultaneous detection of multiple SNPs in one single reaction (TaqMan Assay, Life Technologies, Carlsbad, CA, USA). Derived methods may also be used, such as the so‐called displacing probes (two allele‐specific probes of different length) and minor groove‐binding probes techniques, both being used for RBC genotyping .…”

Section: Medium‐ To High‐throughput Systemsmentioning

confidence: 99%

mentioning

confidence: 99%

Molecular tools for investigating immunohaematology problems

Peyrard

2015

ISBT Science Series

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Blood types are classically determined by haemagglutination methods. However, several backgrounds represent limitations for serological techniques, which may be overcome by molecular testing, for example blood typing in recently transfused patients or in case of a positive IgG direct antiglobulin test when indirect antiglobulin testing is required for antisera. A multitude of molecular tools are available and can be separated in five groups: low‐throughput tests, medium‐ to high‐throughput assays, genomic DNA sequencing, high‐throughput DNA sequencing devices and other specialized techniques. The low‐throughput tests especially include PCR‐restriction fragment length polymorphism (RFLP) and manual allele‐specific PCR techniques. The medium‐to high‐throughput systems, mainly real‐time PCR, microarray DNA chips and mass spectrometry, allow for the testing of multiple different SNPs. The so‐called ‘next‐generation sequencing’ technologies can analyse 10 Mb to 1000 Gb per run. Their implementation in transfusion medicine is currently developed by a few teams worldwide, and they may become a major investigating tool in the next future. Standard genomic DNA sequencing may be used when genotyping assays are unavailable or when screening for common mutations by genotyping devices is inconclusive. Specialized techniques, reserved for complex cases or research, are performed after messenger RNA extraction, followed by reverse transcription into complementary DNA, which may be directly sequenced or cloned with plasmid vectors for single‐allele sequencing. Finally, electronic databases are also very helpful tools in molecular immunohaematology, for example the resources from the National Center for Biotechnology Information (NCBI), Rhesus Base and the ISBT Website of the Working Party on Red Cell Immunogenetics and Blood Group Terminology.

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MicroRNA (miRNA) Profiling

Jiang

2016

Methods in Molecular Biology

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MicroRNAs (miRNAs) are small, highly conserved noncoding RNA molecules involved in the regulation of gene expression. Since each miRNA regulates the expression of hundreds of target mRNAs, miRNAs could function as master coordinators, efficiently regulating fundamental cellular processes, including proliferation, apoptosis, and development. Furthermore, miRNAs may provide useful diagnostic and therapeutic targets in a variety of diseases. However, miRNA expression profiling is essential for the investigation of the biological functions and clinical applications of miRNAs. Therefore, in this chapter, we review and discuss commonly used techniques for miRNAs profiling, as well as their advantages and restrictions.

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A New Method for Analyzing the Duffy Blood Group Genotype by TaqMan Minor Groove Binding Probes

Abstract: The 5'-NA for genotyping of Duffy blood group is simple, rapid, reliable, reproducible, sensitive, and high-throughput and is superior to PCR-ASP used in routine genotyping.

Cited by 5 publications

References 26 publications

Duffy Blood Group Genotyping in Thai Blood Donors

Duffy Blood Group Genotyping in Thai Blood Donors

Molecular tools for investigating immunohaematology problems

MicroRNA (miRNA) Profiling

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