Duffy Blood Group Genotyping in Thai Blood Donors

Abstract

BackgroundDuffy (FY) blood group genotyping is important in transfusion medicine because Duffy alloantibodies are associated with delayed hemolytic transfusion reactions and hemolytic disease of the fetus and newborn. In this study, FY allele frequencies in Thai blood donors were determined by in-house PCR with sequence-specific primers (PCR-SSP), and the probability of obtaining compatible blood for alloimmunized patients was assessed.MethodsFive hundred blood samples from Thai blood donors of the National Blood Centre, Thai Red Cross Society, were included. Only 200 samples were tested with anti-Fya and anti-Fyb using the gel technique. All 500 samples and four samples from a Guinea family with the Fy(a-b-) phenotype were genotyped by using PCR-SSP. Additionally, the probability of obtaining antigen-negative red blood cells (RBCs) for alloimmunized patients was calculated according to the estimated FY allele frequencies.ResultsThe FY phenotyping and genotyping results were in 100% concordance. The allele frequencies of FY*A and FY*B in 500 central Thais were 0.962 (962/1,000) and 0.038 (38/1,000), respectively. Although the Fy(a-b-) phenotype was not observed in this study, FY*BES/FY*BES was identified by PCR-SSP in the Guinea family and was confirmed by DNA sequencing.ConclusionsOur results confirm the high frequency of the FY*A allele in the Thai population, similar to that of Asian populations. At least 500 Thai blood donors are needed to obtain two units of antigen-negative RBCs for the Fy(a-b+) phenotype.

Distribution of the Duffy genotypes in Malaysian Borneo and its relation to Plasmodium knowlesi malaria susceptibility

“…Our previous study on the Duffy blood group in Peninsular Malaysia was in agreement with this trend, indicating an overwhelmingly FY*A dominant distribution with allelic frequencies of 0.937 (94%) and FY*A/FY*A being the dominant genotype at 89.2% [19]. This allelic and genotypic distribution was similar to those of neighbouring countries such as Thailand [29, 30], Indonesia (Bugis, Toraja, and Irian Jaya area) [31], Philippines [32], Taiwan [32], and China [33, 34]. With our previous study on the Duffy genotypes in Peninsular Malaysia in mind, we sought to determine if similar distributions were observed in Malaysian Borneo or if the distributions would differ due to the geographic separation of the Borneo island.…”

Application of Multiplex Ligation-Dependent Probe Amplification Assay for Genotyping Major Blood Group Systems Including DEL Variants in the D-Negative Korean Population

“…In the Duffy blood group system, two Fy a -negative cases were encountered, similar to previous results [ 33 ]. The frequency of the Fy a -negative phenotype in the Korean population is very low compared with that in the European population, and it is extremely difficult to find Fy a -negative RBCs for anti-Fya alloimmunized patients [ 34 35 ]. Other relevant antigens, including Lutheran, Kell, Yt, Scianna, Colton, LW, Gerbich, Cromer, Knops, Indian, and Ok blood group systems, are not polymorphic in the Korean D-negative individuals.…”

Blood group genotyping in alloimmunized multi‐transfused thalassemia patients from Iran

“…To check the accuracy of protocols, 10 DNA samples, previously genotyped by PCR‐SSP and PCR‐RFLP, and all samples with undetermined phenotype and discrepancies between phenotype and genotype results were sequenced for related exons in all studied blood group systems. For KEL, Kidd, and FY sequencing, the PCR amplification was performed using primers and amplification conditions as described in the previous studies (Jalali et al, 2020 ; Lee et al, 1995 ; Nathalang et al, 2015 ). The Duffy silencing mutation (−67T>C) in the GATA‐1‐binding site at the promoter region of the FY gene (FY*null01) was also screened using primers, and the PCR conditions were identical to those previously described.…”